基于微流控芯片的外泌体分离与检测

匡晶晶 康璐 林楚慧 王月荣 张敏 章弘扬 胡坪

引用本文: 匡晶晶, 康璐, 林楚慧, 王月荣, 张敏, 章弘扬, 胡坪. 基于微流控芯片的外泌体分离与检测[J]. 分析化学, 2021, 49(9): 1437-1450. doi: 10.19756/j.issn.0253-3820.201568 shu
Citation:  KUANG Jing-Jing,  KANG Lu,  LIN Chu-Hui,  WANG Yue-Rong,  ZHANG Min,  ZHANG Hong-Yang,  Hu Ping. Separation and Detection of Exosomes Based on Microfluidic Chip[J]. Chinese Journal of Analytical Chemistry, 2021, 49(9): 1437-1450. doi: 10.19756/j.issn.0253-3820.201568 shu

基于微流控芯片的外泌体分离与检测

    通讯作者: 胡坪,E-mail:huping@ecust.edu.cn
  • 基金项目:

    国家自然科学基金项目(No.81973285)资助。

摘要: 外泌体作为最小的细胞外囊泡,内含有脂质、蛋白质、mRNA、microRNA以及双链DNA,可被大多数细胞分泌并在体液中循环,参与细胞间的通讯。近年的研究发现,外泌体在肿瘤发生、迁移、生长、组织损伤修复、免疫抗原呈递及神经退行性疾病中发挥了极其重要的作用,可作为相关疾病诊断的生物标志物,因此,外泌体的分离和检测技术的研究至关重要。本文总结了近年来基于微流控芯片的外泌体分离和检测方法的研究进展。

English


    1. [1]

      MELDOLESI J. Curr. Biol., 2018, 28(8):435-444.MELDOLESI J. Curr. Biol., 2018, 28(8):435-444.

    2. [2]

      DOYLE L M, WANG M Z. Cells, 2019, 8(7):727.DOYLE L M, WANG M Z. Cells, 2019, 8(7):727.

    3. [3]

      CONTRERASNARANJO J C, WU H, UGAZ V M. Lab Chip, 2017, 17(21):3558-3577.CONTRERASNARANJO J C, WU H, UGAZ V M. Lab Chip, 2017, 17(21):3558-3577.

    4. [4]

      JOHNSTONE R M, ADAM M, HAMMOND J R, ORR L, TURBIDE C. J. Biol. Chem., 1987, 262(19):9412-9420.JOHNSTONE R M, ADAM M, HAMMOND J R, ORR L, TURBIDE C. J. Biol. Chem., 1987, 262(19):9412-9420.

    5. [5]

      TORO J D, HERSCHLIK L, WALDNER C, MONGINI C. Front. Immunol., 2015, 6:203.TORO J D, HERSCHLIK L, WALDNER C, MONGINI C. Front. Immunol., 2015, 6:203.

    6. [6]

      KOWAL J, TKACH M, THERY C. Curr. Opin. Cell Biol., 2014, 29(29):116-125.KOWAL J, TKACH M, THERY C. Curr. Opin. Cell Biol., 2014, 29(29):116-125.

    7. [7]

      LIN S J, YU Z X, CHEN D, WANG Z G, MIAO J M, LI Q C, ZHANG D Y, SONG J, CUI D X. Small, 2020, 16(9):1903916.LIN S J, YU Z X, CHEN D, WANG Z G, MIAO J M, LI Q C, ZHANG D Y, SONG J, CUI D X. Small, 2020, 16(9):1903916.

    8. [8]

      GURUNATHAN S, KANG M, JEYARAJ M, QASIM M, KIM J. Cells, 2019, 8(4):307.GURUNATHAN S, KANG M, JEYARAJ M, QASIM M, KIM J. Cells, 2019, 8(4):307.

    9. [9]

      BATISTA B S, ENG W S, PILOBELLO K T, HENDRICKSMUNOZ K D, MAHAL L K. J. Proteome Res., 2011, 10(10):4624-4633.BATISTA B S, ENG W S, PILOBELLO K T, HENDRICKSMUNOZ K D, MAHAL L K. J. Proteome Res., 2011, 10(10):4624-4633.

    10. [10]

      THAKUR B K, ZHANG H Y, BECKER A, MATEI I, HUANG Y J, COSTASILVA B, ZHENG Y, HOSHINO A, BRAZIER H, XIANG J, WILLIAMS C, RODRIGUEZBARRUECO R, SILVA J M, ZHANG W J, HEARN S, ELEMENTO O, PAKNEJAD N, MANOVATODOROVA K, WELTE K, BROMBERG J, PEINADO H, LYDEN D. Cell Res., 2014, 24(6):766-769.THAKUR B K, ZHANG H Y, BECKER A, MATEI I, HUANG Y J, COSTASILVA B, ZHENG Y, HOSHINO A, BRAZIER H, XIANG J, WILLIAMS C, RODRIGUEZBARRUECO R, SILVA J M, ZHANG W J, HEARN S, ELEMENTO O, PAKNEJAD N, MANOVATODOROVA K, WELTE K, BROMBERG J, PEINADO H, LYDEN D. Cell Res., 2014, 24(6):766-769.

    11. [11]

      DENG X, RUAN H Y, ZHANG X J, XU X, ZHU Y, PENG H X, ZHANG X M, KONG F Y, GUAN M. Int. J. Cancer, 2020, 146(6):1700-1716.DENG X, RUAN H Y, ZHANG X J, XU X, ZHU Y, PENG H X, ZHANG X M, KONG F Y, GUAN M. Int. J. Cancer, 2020, 146(6):1700-1716.

    12. [12]

      KALLURI R, LEBLEU V S. Science, 2020, 367:6478.KALLURI R, LEBLEU V S. Science, 2020, 367:6478.

    13. [13]

      CONSOLE L, SCALISE M, INDIVERI C. Clin. Chim. Acta, 2019, 488:165-171.CONSOLE L, SCALISE M, INDIVERI C. Clin. Chim. Acta, 2019, 488:165-171.

    14. [14]

      NAHAND J S, MOGHOOFEI M, SALMANINEJAD A, BAHMANPOUR Z, KARIMZADEH M, NASIRI M, MIRZAEI H R, POURHANIFEH M H, BOKHARAEISALIM F, MIRZAEI H, HAMBLIN M R. Int. J. Cancer, 2020, 146(2):305-320.NAHAND J S, MOGHOOFEI M, SALMANINEJAD A, BAHMANPOUR Z, KARIMZADEH M, NASIRI M, MIRZAEI H R, POURHANIFEH M H, BOKHARAEISALIM F, MIRZAEI H, HAMBLIN M R. Int. J. Cancer, 2020, 146(2):305-320.

    15. [15]

      CHEN G, HUANG A C, ZHANG W, ZHANG G, WU M, XU W, YU Z L, YANG J G, WANG B K, SUN H, XIA H F, MAN Q W, ZHONG W G, ANTELO L F, WU B, XIONG X P, LIU X M, GUAN L, LI T, LIU S J, YANG R F, LU Y T, DONG L Y, MCGETTIGAN S, SOMASUNDARAM R, RADHAKRISHNAN R, MILLS G B, LU Y L, KIM J, CHEN Y H, DONG H D, ZHAO Y F, KARAKOUSIS G C, MITCHELL T C, SCHUCHTER L M, HERLYN M, WHERRY E J, XU X W, GUO W. Nature, 2018, 560(7718):382-386.CHEN G, HUANG A C, ZHANG W, ZHANG G, WU M, XU W, YU Z L, YANG J G, WANG B K, SUN H, XIA H F, MAN Q W, ZHONG W G, ANTELO L F, WU B, XIONG X P, LIU X M, GUAN L, LI T, LIU S J, YANG R F, LU Y T, DONG L Y, MCGETTIGAN S, SOMASUNDARAM R, RADHAKRISHNAN R, MILLS G B, LU Y L, KIM J, CHEN Y H, DONG H D, ZHAO Y F, KARAKOUSIS G C, MITCHELL T C, SCHUCHTER L M, HERLYN M, WHERRY E J, XU X W, GUO W. Nature, 2018, 560(7718):382-386.

    16. [16]

      ZHONG D, CAO Y, LI C J, LI M, RONG Z J, JIANG L Y, GUO Z, LU H B, HU J Z. Exp. Biol. Med., 2020, 245(1):54-65.ZHONG D, CAO Y, LI C J, LI M, RONG Z J, JIANG L Y, GUO Z, LU H B, HU J Z. Exp. Biol. Med., 2020, 245(1):54-65.

    17. [17]

      MA T, CHEN Y Q, CHEN Y H, MENG Q Y, SUN J C, SHAO L B, YU YS, HUANG H Y, HU Y Q, YANG Z Y, YANG J J, SHEN Z Y. Stem. Cells Int., 2018:3290372.MA T, CHEN Y Q, CHEN Y H, MENG Q Y, SUN J C, SHAO L B, YU YS, HUANG H Y, HU Y Q, YANG Z Y, YANG J J, SHEN Z Y. Stem. Cells Int., 2018:3290372.

    18. [18]

      KAKARLA R, HUR J, KIM Y J, KIM J, CHWAE Y. Exp. Mol. Med., 2020, 52(1):1-6.KAKARLA R, HUR J, KIM Y J, KIM J, CHWAE Y. Exp. Mol. Med., 2020, 52(1):1-6.

    19. [19]

      SARKO D K, MCKINNEY C E. Front. NeuroSci., 2017, 82:47.SARKO D K, MCKINNEY C E. Front. NeuroSci., 2017, 82:47.

    20. [20]

      HOSHINO A, COSTASILVA B, SHEN T, RODRIGUES G, HASHIMOTOA, MARK M T, MOLINA H, KOHSAKA S, GIANNATALE A D, CEDER S, SINGH S, WILLIAMS C, SOPLOP N, URYU K, PHARMER L A, KING T A, BOJMAR L, DAVIES A E, ARARSO Y, ZHANG T, ZHANG H Y, HERNANDEZ J M, WEISS J M, DUMONTCOLE V D, KRAMER K, WEXLER L H, NARENDRAN A, SCHWARTZ G K, HEALEY J H, SANDSTROM P, LABORI K J, KURE E H, GRANDGENETT P M, HOLLINGSWORTH M A, SOUSA M D, KAUR S, JAIN M, MALLYA K, BATRA S K, JARNAGIN W R, BRADY M S, FODSTAD O, MULLER V, PANTEL K, MINN A J, BISSELL M J, GARCIA B A, KANG Y B, RAJASEKHAR V K, GHAJAR C M, MATEI I, PEINADO H, BROMBERG J, LYDEN D. Nature, 2015, 527(7578):329-335.HOSHINO A, COSTASILVA B, SHEN T, RODRIGUES G, HASHIMOTOA, MARK M T, MOLINA H, KOHSAKA S, GIANNATALE A D, CEDER S, SINGH S, WILLIAMS C, SOPLOP N, URYU K, PHARMER L A, KING T A, BOJMAR L, DAVIES A E, ARARSO Y, ZHANG T, ZHANG H Y, HERNANDEZ J M, WEISS J M, DUMONTCOLE V D, KRAMER K, WEXLER L H, NARENDRAN A, SCHWARTZ G K, HEALEY J H, SANDSTROM P, LABORI K J, KURE E H, GRANDGENETT P M, HOLLINGSWORTH M A, SOUSA M D, KAUR S, JAIN M, MALLYA K, BATRA S K, JARNAGIN W R, BRADY M S, FODSTAD O, MULLER V, PANTEL K, MINN A J, BISSELL M J, GARCIA B A, KANG Y B, RAJASEKHAR V K, GHAJAR C M, MATEI I, PEINADO H, BROMBERG J, LYDEN D. Nature, 2015, 527(7578):329-335.

    21. [21]

      LIAO W, DU Y, ZHANG C H, PAN F W, YAO Y, ZHANG T, PENG Q. Acta Biomater., 2019, 86:1-14.LIAO W, DU Y, ZHANG C H, PAN F W, YAO Y, ZHANG T, PENG Q. Acta Biomater., 2019, 86:1-14.

    22. [22]

      MOMENHERAVI F, BALAJ L,ALIAN S, MANTEL P, HALLECK A E, TRACHTENBERG A J, SORIA C E, OQUIN S, BONEBREAK C M, SARACOGLU E, SKOG J, KUO W P. Biol. Chem., 2013, 394(10):1253-1262.MOMENHERAVI F, BALAJ L,ALIAN S, MANTEL P, HALLECK A E, TRACHTENBERG A J, SORIA C E, OQUIN S, BONEBREAK C M, SARACOGLU E, SKOG J, KUO W P. Biol. Chem., 2013, 394(10):1253-1262.

    23. [23]

      GAMEZVALERO A, MONGUIOTORTAJADA M, CARRERASPLANELLA L, FRANQUESA M, BEYER K, BORRAS F E. Sci. Rep., 2016, 6:33641.GAMEZVALERO A, MONGUIOTORTAJADA M, CARRERASPLANELLA L, FRANQUESA M, BEYER K, BORRAS F E. Sci. Rep., 2016, 6:33641.

    24. [24]

      LOBB R J, BECKER M, WEN S W, WONG C S, WIEGMANS A P, LEIMGRUBER A, MOLLER A. J. Extracell. Vesicles, 2015, 4(1):27031.LOBB R J, BECKER M, WEN S W, WONG C S, WIEGMANS A P, LEIMGRUBER A, MOLLER A. J. Extracell. Vesicles, 2015, 4(1):27031.

    25. [25]

      GARDINER C, FERREIRA Y J, DRAGOVIC R A, REDMAN C W, SARGENT I L. J. Extracell. Vesicles, 2013, 2(1):19671.GARDINER C, FERREIRA Y J, DRAGOVIC R A, REDMAN C W, SARGENT I L. J. Extracell. Vesicles, 2013, 2(1):19671.

    26. [26]

      SITAR S, KEJŽAR A, PAHOVNIK D, KOGEJ K, TUŠEK-ŽNIDARIC M, LENASSI M, ŽAGAR E. Anal. Chem., 2015, 87(18):9225-9233.SITAR S, KEJŽAR A, PAHOVNIK D, KOGEJ K, TUŠEK-ŽNIDARIC M, LENASSI M, ŽAGAR E. Anal. Chem., 2015, 87(18):9225-9233.

    27. [27]

      SHAO H, CHUNG J, BALAJ L, CHAREST A, BIGNER D D, CARTER B S, HOCHBERG F H, BREAKEFIELD X O, WEISSLEDER R, LEE H. Nat. Med., 2012, 18(12):1835-1840.SHAO H, CHUNG J, BALAJ L, CHAREST A, BIGNER D D, CARTER B S, HOCHBERG F H, BREAKEFIELD X O, WEISSLEDER R, LEE H. Nat. Med., 2012, 18(12):1835-1840.

    28. [28]

      STREET J M, BARRAN P E, MACKAY C L, WEIDT S, BALMFORTH C, WALSH T S, CHALMERS R T, WEBB D J, DEAR J W. J. Transl. Med., 2012, 10(1):1-7.STREET J M, BARRAN P E, MACKAY C L, WEIDT S, BALMFORTH C, WALSH T S, CHALMERS R T, WEBB D J, DEAR J W. J. Transl. Med., 2012, 10(1):1-7.

    29. [29]

      WHITEHEAD B, WU L, HVAM M L, ASLAN H, DONG M, DYRSKJØT L, OSTENFELD M S, MOGHIMI S M, HOWARD K A. J. Extracell. Vesicles, 2015, 4(1):29685.WHITEHEAD B, WU L, HVAM M L, ASLAN H, DONG M, DYRSKJØT L, OSTENFELD M S, MOGHIMI S M, HOWARD K A. J. Extracell. Vesicles, 2015, 4(1):29685.

    30. [30]

      POSPICHALOVA V, SVOBODA J, DAVE Z, KOTRBOVA A, KAISER K, KLEMOVA D, ILKOVICS L, HAMPL A, CRHA I, JANDAKOVA E, MINAR L, WEINBERGER V, BRYJA V. J. Extracell. Vesicles, 2015, 4(1):25530.POSPICHALOVA V, SVOBODA J, DAVE Z, KOTRBOVA A, KAISER K, KLEMOVA D, ILKOVICS L, HAMPL A, CRHA I, JANDAKOVA E, MINAR L, WEINBERGER V, BRYJA V. J. Extracell. Vesicles, 2015, 4(1):25530.

    31. [31]

      DAVIES R T, KIM J, JANG S C, CHOI E, GHO Y S, PARK J. Lab Chip, 2012, 12(24):5202-5210.DAVIES R T, KIM J, JANG S C, CHOI E, GHO Y S, PARK J. Lab Chip, 2012, 12(24):5202-5210.

    32. [32]

      WOO H, SUNKARA V, PARK J, KIM T, HAN J, KIM C, CHOI H, KIM Y, CHO Y. ACS Nano, 2017, 11(2):1360-1370.WOO H, SUNKARA V, PARK J, KIM T, HAN J, KIM C, CHOI H, KIM Y, CHO Y. ACS Nano, 2017, 11(2):1360-1370.

    33. [33]

      LIANG L G, KONG M Q, ZHOU S, SHENG Y F, WANG P, YU T, INCI F, KUO W P, LI L J, DEMIRCI U, WANG S Q. Sci. Rep., 2017, 7:46224.LIANG L G, KONG M Q, ZHOU S, SHENG Y F, WANG P, YU T, INCI F, KUO W P, LI L J, DEMIRCI U, WANG S Q. Sci. Rep., 2017, 7:46224.

    34. [34]

      LIU F, VERMESH O, MANI V, GE T J, MADSEN S, SABOUR A, HSU E, GOWRISHANKAR G, KANADA M, JOKERST J V, SIERRA R G, CHANG E, LAU K, SRIDHAR K, BERMUDEZ A, PITTERI S J, STOYANOVA T, SINCLAIR R, NAIR V S, GAMBHIR S S, DEMIRCI U. ACS Nano, 2017, 11(11):10712-10723.LIU F, VERMESH O, MANI V, GE T J, MADSEN S, SABOUR A, HSU E, GOWRISHANKAR G, KANADA M, JOKERST J V, SIERRA R G, CHANG E, LAU K, SRIDHAR K, BERMUDEZ A, PITTERI S J, STOYANOVA T, SINCLAIR R, NAIR V S, GAMBHIR S S, DEMIRCI U. ACS Nano, 2017, 11(11):10712-10723.

    35. [35]

      WANG Z X, WU H, FINE D, SCHMULEN J, HU Y, GODIN B, ZHANG J X J, LIU X W. Lab Chip, 2013, 13(15):2879-2882.WANG Z X, WU H, FINE D, SCHMULEN J, HU Y, GODIN B, ZHANG J X J, LIU X W. Lab Chip, 2013, 13(15):2879-2882.

    36. [36]

      YOKOYAMA A, YASUI T, GODA T, YANAGIDA T, TANAKA M, MUTO M, OKOCHI M, KAJI N, KANAI M, NAGASHIMA K, MIYAHARA Y, KAWAI T, BABA Y. MicroTAS 2016, Dublin, 2016:1156-1157.YOKOYAMA A, YASUI T, GODA T, YANAGIDA T, TANAKA M, MUTO M, OKOCHI M, KAJI N, KANAI M, NAGASHIMA K, MIYAHARA Y, KAWAI T, BABA Y. MicroTAS 2016, Dublin, 2016:1156-1157.

    37. [37]

      NEMATI Z, UM J, KOUHPANJI M R Z, ZHOU F, GAGE T, SHORE D, MAKIELSKI K, DONNELLY A, ALONSO J. ACS Appl. Nano Mater., 2020, 3(2):2058-2069.NEMATI Z, UM J, KOUHPANJI M R Z, ZHOU F, GAGE T, SHORE D, MAKIELSKI K, DONNELLY A, ALONSO J. ACS Appl. Nano Mater., 2020, 3(2):2058-2069.

    38. [38]

      SANTANA S M, ANTONYAK M A, CERIONE R A, KIRBY B. Biomed. Microdevices, 2014, 16(6):869-877.SANTANA S M, ANTONYAK M A, CERIONE R A, KIRBY B. Biomed. Microdevices, 2014, 16(6):869-877.

    39. [39]

      WUNSCH B H, SMITH J T, GIFFORD S M, WANG CHAO, BRINK M, BRUCE L R, AUSTIN R H, STOLOVITZKY G, ASTIER A. Nat. Nanotechnol., 2016, 11(11):936-940.WUNSCH B H, SMITH J T, GIFFORD S M, WANG CHAO, BRINK M, BRUCE L R, AUSTIN R H, STOLOVITZKY G, ASTIER A. Nat. Nanotechnol., 2016, 11(11):936-940.

    40. [40]

      DESHMUKH S, BRZOZKA Z, LAURELL T, AUGUSTSSON P. Lab Chip, 2014, 14(17):3394-3400.DESHMUKH S, BRZOZKA Z, LAURELL T, AUGUSTSSON P. Lab Chip, 2014, 14(17):3394-3400.

    41. [41]

      GAUTAM G P, GURUNG R, FENCL F A, PIYASENA M E. Anal. Bioanal. Chem., 2018, 410(25):6561-6571.GAUTAM G P, GURUNG R, FENCL F A, PIYASENA M E. Anal. Bioanal. Chem., 2018, 410(25):6561-6571.

    42. [42]

      LEE K, SHAO H, WEISSLEDER R, LEE H. ACS Nano, 2015, 9(3):2321-2327.LEE K, SHAO H, WEISSLEDER R, LEE H. ACS Nano, 2015, 9(3):2321-2327.

    43. [43]

      WU M X, OUYANG Y, WANG Z Y, ZHANG R, HUANG P, CHEN C Y, LI H, LI P, QUINN D, DAO M, SURESH S, SADOVSKY Y, HUANG T J. Proc. Natl. Acad. Sci. U. S. A., 2017, 114(40):10584-10589.WU M X, OUYANG Y, WANG Z Y, ZHANG R, HUANG P, CHEN C Y, LI H, LI P, QUINN D, DAO M, SURESH S, SADOVSKY Y, HUANG T J. Proc. Natl. Acad. Sci. U. S. A., 2017, 114(40):10584-10589.

    44. [44]

      LIU C, GUO J Y, TIAN F, YANG N, YAN F S, DING Y P, WEI J Y, HU G Q, NIE G J, SUN J S. ACS Nano, 2017, 11(7):6968-6976.LIU C, GUO J Y, TIAN F, YANG N, YAN F S, DING Y P, WEI J Y, HU G Q, NIE G J, SUN J S. ACS Nano, 2017, 11(7):6968-6976.

    45. [45]

      IBSEN S D, WRIGHT J, LEWIS J M, KIM S, KO S Y, ONG J, MANOUCHEHRI S, VYAS A, AKERS J C, CHEN C C, CARTER B S, ESENER S C, HELLER M J. ACS Nano, 2017, 11(7):6641-6651.IBSEN S D, WRIGHT J, LEWIS J M, KIM S, KO S Y, ONG J, MANOUCHEHRI S, VYAS A, AKERS J C, CHEN C C, CARTER B S, ESENER S C, HELLER M J. ACS Nano, 2017, 11(7):6641-6651.

    46. [46]

      LEWIS J M, VYAS A, QIU YU-QI, MESSER K, WHITE R R, HELLER M J. ACS Nano, 2018, 12(4):3311-3320.LEWIS J M, VYAS A, QIU YU-QI, MESSER K, WHITE R R, HELLER M J. ACS Nano, 2018, 12(4):3311-3320.

    47. [47]

      AGHILINEJAD A, AGHAAMOO M, CHEN X L, XU J. Electrophoresis, 2018, 39:869-877.AGHILINEJAD A, AGHAAMOO M, CHEN X L, XU J. Electrophoresis, 2018, 39:869-877.

    48. [48]

      CHEN C, SKOG J, HSU C, LESSARD R T, BALAJ L, WURDINGER T, CARTER B S, BREAKEFIELD X O, TONER M, IRIMIA D. Lab Chip, 2010, 10(4):505-511.CHEN C, SKOG J, HSU C, LESSARD R T, BALAJ L, WURDINGER T, CARTER B S, BREAKEFIELD X O, TONER M, IRIMIA D. Lab Chip, 2010, 10(4):505-511.

    49. [49]

      KANWAR S S, DUNLAY C J, SIMEONE D M, NAGRATH S. Lab Chip, 2014, 14(11):1891-1900.KANWAR S S, DUNLAY C J, SIMEONE D M, NAGRATH S. Lab Chip, 2014, 14(11):1891-1900.

    50. [50]

      CHEN C, LIN B, WANG H, FAN S, HSU M, CHENG C. Microfluid. Nanofluid., 2014, 16(5):849-856.CHEN C, LIN B, WANG H, FAN S, HSU M, CHENG C. Microfluid. Nanofluid., 2014, 16(5):849-856.

    51. [51]

      ZHANG P, HE M, ZENG Y. Lab Chip, 2016, 16(16):3033-3042.ZHANG P, HE M, ZENG Y. Lab Chip, 2016, 16(16):3033-3042.

    52. [52]

      KANG Y, PURCELL E, PALACIOSROLSTON C, LO T, RAMNATH N, JOLLY S, NAGRATH S. Small, 2019, 15(47):1903600.KANG Y, PURCELL E, PALACIOSROLSTON C, LO T, RAMNATH N, JOLLY S, NAGRATH S. Small, 2019, 15(47):1903600.

    53. [53]

      TAYEBI M, ZHOU Y N, TRIPATHI P, CHANDRAMOHANADAS R, AI Y. Anal. Chem., 2020, 92(15):10733-10742.TAYEBI M, ZHOU Y N, TRIPATHI P, CHANDRAMOHANADAS R, AI Y. Anal. Chem., 2020, 92(15):10733-10742.

    54. [54]

      HE M, CROW J, ROTH M, ZENG Y, GODWIN A K. Lab Chip, 2014, 14(19):3773-3780.HE M, CROW J, ROTH M, ZENG Y, GODWIN A K. Lab Chip, 2014, 14(19):3773-3780.

    55. [55]

      ZHAO Z, YANG Y, ZENG Y, HE M. Lab Chip, 2016, 16(3):489-496.ZHAO Z, YANG Y, ZENG Y, HE M. Lab Chip, 2016, 16(3):489-496.

    56. [56]

      XU H Y, LIAO C, ZUO P, LIU Z W, YE B. Anal. Chem., 2018, 90(22):13451-13458.XU H Y, LIAO C, ZUO P, LIU Z W, YE B. Anal. Chem., 2018, 90(22):13451-13458.

    57. [57]

      NIU F Z, CHEN X F, NIU X M, CAI Y F, ZHANG Q K, CHEN T, YANG H. Micromachines, 2020, 11(5):503.NIU F Z, CHEN X F, NIU X M, CAI Y F, ZHANG Q K, CHEN T, YANG H. Micromachines, 2020, 11(5):503.

    58. [58]

      DUDANI J S, GOSSETT D R, TSE H T, LAMM R J, KULKARNI R P, CARLO D D. Biomicrofluidics, 2015, 9(1):014112.DUDANI J S, GOSSETT D R, TSE H T, LAMM R J, KULKARNI R P, CARLO D D. Biomicrofluidics, 2015, 9(1):014112.

    59. [59]

      FANG S M, TIAN H Z, LI X C,JIN X J, JIN D, LI X J, KONG J, YANG C, YANG X S, LU Y, LIN B C, NIU W D, LIU T J. Plos One, 2017, 12(4):0175050.FANG S M, TIAN H Z, LI X C,JIN X J, JIN D, LI X J, KONG J, YANG C, YANG X S, LU Y, LIN B C, NIU W D, LIU T J. Plos One, 2017, 12(4):0175050.

    60. [60]

      FRIEDRICH R, BLOCK S, ALIZADEHHEIDARI M, HEIDER S, FRITZSCHE J, ESBJORNER E K, WESTERLUND F, BALLY M. Lab Chip, 2017, 17(5):830-841.FRIEDRICH R, BLOCK S, ALIZADEHHEIDARI M, HEIDER S, FRITZSCHE J, ESBJORNER E K, WESTERLUND F, BALLY M. Lab Chip, 2017, 17(5):830-841.

    61. [61]

      HISEY C L, DORAYAPPAN K D, COHN D E, SELVENDIRAN K, HANSFORD D J. Lab Chip, 2018, 18(20):3144-3153.HISEY C L, DORAYAPPAN K D, COHN D E, SELVENDIRAN K, HANSFORD D J. Lab Chip, 2018, 18(20):3144-3153.

    62. [62]

      JIANG Y, SHI M L, LIU Y, WAN W, CUI C, ZHANG L Q, TAN W H. Angew. Chem., Int. Ed., 2017, 56(39):11916-11920.JIANG Y, SHI M L, LIU Y, WAN W, CUI C, ZHANG L Q, TAN W H. Angew. Chem., Int. Ed., 2017, 56(39):11916-11920.

    63. [63]

      DI H X, MI Z, SUN Y, LIU X H, LIU X Z, LI A, JIANG Y, GAO H M, RONG P F, LIU D B. Theranostics, 2020, 10(20):9303-9314.DI H X, MI Z, SUN Y, LIU X H, LIU X Z, LI A, JIANG Y, GAO H M, RONG P F, LIU D B. Theranostics, 2020, 10(20):9303-9314.

    64. [64]

      CHEN Z, CHENG S B, CAO P, QIU Q F,CHEN Y,XIE M,XU Y,HUANG W H. Biosens. Bioelectron., 2018, 122:211-216.CHEN Z, CHENG S B, CAO P, QIU Q F,CHEN Y,XIE M,XU Y,HUANG W H. Biosens. Bioelectron., 2018, 122:211-216.

    65. [65]

      FRAIKIN J, TEESALU T, MCKENNEY C M, RUOSLAHTI E, CLELAND A N. Nat. Nanotechnol., 2011, 6(5):308-313.FRAIKIN J, TEESALU T, MCKENNEY C M, RUOSLAHTI E, CLELAND A N. Nat. Nanotechnol., 2011, 6(5):308-313.

    66. [66]

      SHIN S R, KILIC T, ZHANG Y S, AVCI H, HU N, KIM D, BRANCO C, ALEMAN J, MASSA S, SILVESTRI A, KANG J, DESALVO A, HUSSAINI A M, CHAE S K, POLINI A, BHISE N S, HUSSAIN M A, LEE H, DOKMECI M R, KHADEMHOSSEINI A. Adv. Sci., 2017, 4(5):1600522.SHIN S R, KILIC T, ZHANG Y S, AVCI H, HU N, KIM D, BRANCO C, ALEMAN J, MASSA S, SILVESTRI A, KANG J, DESALVO A, HUSSAINI A M, CHAE S K, POLINI A, BHISE N S, HUSSAIN M A, LEE H, DOKMECI M R, KHADEMHOSSEINI A. Adv. Sci., 2017, 4(5):1600522.

    67. [67]

      ZHOU Q, RAHIMIAN A, SON K J, SHIN D, PATEL T C, REVZIN A. Methods, 2016, 97(97):88-93.ZHOU Q, RAHIMIAN A, SON K J, SHIN D, PATEL T C, REVZIN A. Methods, 2016, 97(97):88-93.

    68. [68]

      TSANG D K, LIEBERTHAL T J, WATTS C, DUNLOP I E, RAMADAN S, HERNANDEZ A D R, KLEIN N. Sci. Rep., 2019, 9:13946.TSANG D K, LIEBERTHAL T J, WATTS C, DUNLOP I E, RAMADAN S, HERNANDEZ A D R, KLEIN N. Sci. Rep., 2019, 9:13946.

    69. [69]

      ZHU L, WANG K, CUI J, LIU H, BU X L, MA H L, WANG W Z, GONG H, LAUSTED C, HOOD L, YANG G, HU Z Y. Anal. Chem., 2014, 86(17):8857-8864.ZHU L, WANG K, CUI J, LIU H, BU X L, MA H L, WANG W Z, GONG H, LAUSTED C, HOOD L, YANG G, HU Z Y. Anal. Chem., 2014, 86(17):8857-8864.

    70. [70]

      BATHINI S, RAJU D, BADILESCU S, PACKIRISAMY M. Int. J. Biomed. Biol. Eng., 2018, 12(5):236-239.BATHINI S, RAJU D, BADILESCU S, PACKIRISAMY M. Int. J. Biomed. Biol. Eng., 2018, 12(5):236-239.

    71. [71]

      TIAN Y F, NING C F, HE F, YIN B C, YE B C. Analyst, 2018, 143(20):4915-4922.TIAN Y F, NING C F, HE F, YIN B C, YE B C. Analyst, 2018, 143(20):4915-4922.

    72. [72]

      KWIZERA E A, O'CONNOR R, VINDUSKA V, WILLIAMS M, BUTCH E R, SNYDER S E, CHEN X, HUANG X H. Theranostics, 2018, 8(10):2722-2738.KWIZERA E A, O'CONNOR R, VINDUSKA V, WILLIAMS M, BUTCH E R, SNYDER S E, CHEN X, HUANG X H. Theranostics, 2018, 8(10):2722-2738.

    73. [73]

      WANG Y L, LI Q Y, SHI H M, TANG K Q, QIAO L, YU G P, DING C F, YU S N. Lab. Chip, 2020, 20(24):4632-4637.WANG Y L, LI Q Y, SHI H M, TANG K Q, QIAO L, YU G P, DING C F, YU S N. Lab. Chip, 2020, 20(24):4632-4637.

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  • 收稿日期:  2020-09-21
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