Citation: WANG Jia-Qi, WU Jia-Yi, LIN Jian-Yuan, LI Tian-Hua, LI Deng-Feng, GAN Ning. Rapid Detection of Staphylococcus aureus by An Electrochemical Immunosensor Based on Egg Yolk Antibody-Metal Organic Framework Composite Probe[J]. Chinese Journal of Analytical Chemistry, ;2021, 49(2): 197-206. doi: 10.19756/j.issn.0253-3820.201566 shu

Rapid Detection of Staphylococcus aureus by An Electrochemical Immunosensor Based on Egg Yolk Antibody-Metal Organic Framework Composite Probe

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School of Materials Science and Chemical Engineering, Ningbo University, Ningbo 315211, China;
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College of Oceanography, Ningbo University, Ningbo 315211, China;
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College of Biology and Environment, Zhejiang Wanli University, Ningbo 315200, China

Corresponding author: LI Tian-Hua, GAN Ning,
Received Date: 20 September 2020
Revised Date: 25 November 2020

Fund Project: Supported by the National Natural Science Foundation of China (No.21974074), the Zhejiang Provincial Natural Science Foundation of China (Nos.LY19B050001, LY18B070004, 2019A610184), the Zhejiang Basic Public Welfare Program Research Project (Nos.KF2019001, LGN18H300001) and K C Wong Magna Fund in Ningbo University.

Staphylococcus aureus (S.aureus) contamination is an important factor affecting food quality and safety. Therefore, it is urgent to develop a quick and easy on-site analysis method for detecting S.aureus in food. In this work, inactivated S.aureus was used to immunize specific pathogen free (SPF) hens, and the yolk antibody (IgY) was purified from the eggs wich could efficiently and specifically capture S.aureus. IgY modified on the surface of the screen-printed electrode could be used for the selective separation and enrichment of S.aureus in the sample. Ferrocene (Fc) and phenylboronic acid (BPA) were covalently connected on the surface of UiO-66 nano-metal organic framework material (MOF) to obtain a MOF@Fc-BPA composite probe. The prepared probe and the IgY on the electrode jointly identified and captured S.aureus, and then generated a redox signal of Fc on the electrode. Under the optimal reaction conditions, the electrochemical signal intensity was proportional to the logarithm of the S.aureus concentration, the detection range was 10-10⁹ cfu/mL, the detection limit was 3 cfu/mL, and the detection time was 20 min only. In addition, we used this sensor to detect S.aureus in cultured shrimp, and the results obtained were consistent with that of standard ELISA, while the detection time was greatly shortened, and the detection limit was two orders of magnitude lower than the ELISA method. The results indicated that the immunosensor was able to quickly screen S.aureus on site.
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