激光剥蚀-电感耦合等离子体质谱定量铬方法研究

引用本文: 魏星, 周静静, 刘金辉, 陈明丽, 王建华. 激光剥蚀-电感耦合等离子体质谱定量铬方法研究[J]. 分析化学, 2021, 49(3): 432-439. doi: 10.19756/j.issn.0253-3820.201514 shu

Citation: WEI Xing, ZHOU Jing-Jing, LIU Jin-Hui, CHEN Ming-Li, WANG Jian-Hua. Determination of Chromium Based on Laser Ablation Inductively Coupled Plasma Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, 2021, 49(3): 432-439. doi: 10.19756/j.issn.0253-3820.201514 shu

激光剥蚀-电感耦合等离子体质谱定量铬方法研究

东北大学理学院化学系, 沈阳 110819

通讯作者: 陈明丽,E-mail:chenml@mail.nseu.edu.cn; 王建华,E-mail:jianhuajrz@mail.neu.edu.cn

基金项目:
国家自然科学基金项目（Nos.21727811，21675019）资助。

摘要: 建立了基体匹配外标法-激光剥蚀电感耦合等离子体质谱（LA-ICP-MS）定量分析凝胶和细胞中铬含量的方法。对标准品分析的相对标准偏差小于16%（n=300），LA-ICP-MS标准曲线相关系数R²=0.9805，方法的检出限为2.22 μg/g。以MCF-7细胞作为体外细胞模型，经含铬培养基孵育后，用变性和非变性两种聚丙烯酰胺凝胶电泳法（SDS-PAGE和Native-PAGE）分离蛋白提取液，并用LA-ICP-MS法对两种电泳方法得到的电泳条带进行线扫描分析，均检测到含铬蛋白条带。采用LA-ICP-MS对单个细胞中的铬元素进行定量分析与成像，细胞元素分布图与显微镜下的细胞轮廓图重叠良好。

关键词:

English

Determination of Chromium Based on Laser Ablation Inductively Coupled Plasma Mass Spectrometry

Department of Chemistry, College of Science, Northeastern University, Shenyang 110819, China

Corresponding author: CHEN Ming-Li, ; WANG Jian-Hua,

Received Date: 24 August 2020
Revised Date: 22 November 2020
Fund Project:
National Natural Science Foundation of China (Nos. 21727811, 21675019

Abstract: Chromium plays an irreplaceable role in the life system. The exploration of chromium is beneficial to reveal its physiological or pathological processes. In this study, a new method was established for determination of chromium in gels by matrix-matched external standard method-laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). The relative standard deviation of chromium signal intensity during ablation standard was less than 16% (n=300), the correlation coefficient (R²) of LA-ICP-MS quantitative calibration curve was 0.9805, and the detection limit of method was 2.22 μg/g. MCF-7 cells were used as an in vitro cell model, and two kinds of polyacrylamide gel electrophoresis (PAGE) methods were used to separate the protein extract after chromium incubation. And then, the electrophoresis bands obtained by two electrophoresis methods were ablated respectively by LA-ICP-MS with line scan mode, and the weak chromium-containing protein bands were obtained in electrophoresis band. Finally, the LA-ICP-MS was used to measure and image the chromium in individual cell, and it was found that cell element distribution map overlapped well with the cell contour map under the laser microscope.

Key words:

Matrix-matched external standard method
/ Chromium
/ Laser ablation inductively coupled plasma mass spectrometry
/ Gel electrophoresis

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沈阳化工大学材料科学与工程学院沈阳 110142

激光剥蚀-电感耦合等离子体质谱定量铬方法研究

通讯作者: 陈明丽,E-mail:chenml@mail.nseu.edu.cn; 王建华,E-mail:jianhuajrz@mail.neu.edu.cn

English

Determination of Chromium Based on Laser Ablation Inductively Coupled Plasma Mass Spectrometry

Corresponding author: CHEN Ming-Li, ; WANG Jian-Hua,

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

激光剥蚀-电感耦合等离子体质谱定量铬方法研究

通讯作者: 陈明丽,E-mail:chenml@mail.nseu.edu.cn; 王建华,E-mail:jianhuajrz@mail.neu.edu.cn

English

Determination of Chromium Based on Laser Ablation Inductively Coupled Plasma Mass Spectrometry

Corresponding author: CHEN Ming-Li, ; WANG Jian-Hua,

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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通讯作者: 陈斌, bchen63@163.com

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