基于DNAzyme的自驱动DNA分子机器检测有机磷农药

引用本文: 陆伽颀, 叶泰, 徐斐, 曹慧, 袁敏, 于劲松, 阴凤琴, 吴秀秀. 基于DNAzyme的自驱动DNA分子机器检测有机磷农药[J]. 分析化学, 2021, 49(2): 226-236. doi: 10.19756/j.issn.0253-3820.201438 shu

Citation: LU Jia-Qi, YE Tai, XU Fei, CAO Hui, YUAN Min, YU Jin-Song, YIN Feng-Qin, WU Xiu-Xiu. DNAzyme Dependent Self-Powered DNA Machine for Determination of Organophosphorus Pesticides[J]. Chinese Journal of Analytical Chemistry, 2021, 49(2): 226-236. doi: 10.19756/j.issn.0253-3820.201438 shu

基于DNAzyme的自驱动DNA分子机器检测有机磷农药

上海食品快速检测工程技术研究中心, 上海理工大学医疗器械与食品学院, 上海 200093

通讯作者: 叶泰,E-mail:taiye@usst.edu.cn

基金项目:
国家自然科学基金项目（No.31801636）和上海市青年科技英才扬帆计划（No.18YF1417300）资助。

摘要: 通过范德华力作用，将脱氧核酶（DNAzyme）和6-羧基荧光素（FAM）标记的底物链共修饰的金纳米粒子（AuNPs）负载于MnO₂纳米片上，构建了一种自驱动DNA分子机器。乙酰胆碱酯酶（AChE）将其底物乙酰硫代胆碱（ATCh）转化为硫代胆碱（TCh）后，MnO₂纳米片被降解为Mn²⁺，激活DNAzyme切割底物链的循环，使FAM远离AuNPs表面，导致溶液荧光增强。当AChE被有机磷农药（OPs）抑制时，分子机器无法被激活。采用透射电镜（TEM）、紫外-可见吸收光谱、Zeta电位、动态光散射（DLS）和X射线光电子能谱（XPS）对所制备的DNA分子机器进行表征，并对其性能进行优化。在最优条件下，将该探针用于有机磷农药敌敌畏（DDVP）的检测，线性范围为5~500 μg/L，检出限为3.62 μg/L（3σ）。将此方法应用于生菜中DDVP的检测，加标回收率为96.5%~104.2%，相对标准偏差（RSD）为1.7%~7.5%。

关键词:

English

DNAzyme Dependent Self-Powered DNA Machine for Determination of Organophosphorus Pesticides

Shanghai Engineering Research Center for Food Rapid Detection, School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China

Corresponding author: YE Tai, taiye@usst.edu.cn

Received Date: 27 July 2020
Revised Date: 30 December 2020
Fund Project:
Supported by the National Natural Science Foundation of China (No. 31801636) and the Shanghai Sailing Program (No. 18YF1417300).

Abstract: A self-powered DNA machine was constructed by loading DNAzyme strand and dye-labelled substrate strand co-modified gold nanoparticles (AuNPs) on MnO₂ nanosheets via van der Waals force. When acetylcholinesterase (AChE) converted its substrate, acetylthiocholine (ATCh), into thiocholine (TCh), the MnO₂ nanosheets were degraded into Mn²⁺. The Mn²⁺ dependent-DNAzyme was triggered and plenty of dye-labelled fragments were released from the surface of AuNPs, which would lead to the enhancement of fluorescence. When AChE was inhibited by organophosphorus pesticides (OPs), the DNA machine could not be activated. This self-powered DNA machine was characterized by transmission electron microscopy (TEM), ultraviolet-visible absorption spectroscopy, zeta potential, dynamic light scattering (DLS) and X-ray photoelectron spectroscopy (XPS), and the performance of DNA machine was optimized. Under the optimal conditions, the proposed DNA machine was applied to the determination of dichlorvos (DDVP) with a linear range of 5-500 μg/L and a limit of detection of 3.62 μg/L (3σ). The method presented here was applied to the recovery assay of DDVP in spiked lettuce. The recoveries for DDVP were 96.5%-104.2%, and the relative standard deviations (RSD) were 1.7%-7.5%.

Key words:

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沈阳化工大学材料科学与工程学院沈阳 110142

基于DNAzyme的自驱动DNA分子机器检测有机磷农药

通讯作者: 叶泰,E-mail:taiye@usst.edu.cn

English

DNAzyme Dependent Self-Powered DNA Machine for Determination of Organophosphorus Pesticides

Corresponding author: YE Tai, taiye@usst.edu.cn

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

基于DNAzyme的自驱动DNA分子机器检测有机磷农药

通讯作者: 叶泰,E-mail:taiye@usst.edu.cn

English

DNAzyme Dependent Self-Powered DNA Machine for Determination of Organophosphorus Pesticides

Corresponding author: YE Tai, taiye@usst.edu.cn

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

CCS Chemistry：颜徐州、鲍哲南：你的皮肤可能是一片SPMs