基于DNAzyme的自驱动DNA分子机器检测有机磷农药

陆伽颀 叶泰 徐斐 曹慧 袁敏 于劲松 阴凤琴 吴秀秀

引用本文: 陆伽颀, 叶泰, 徐斐, 曹慧, 袁敏, 于劲松, 阴凤琴, 吴秀秀. 基于DNAzyme的自驱动DNA分子机器检测有机磷农药[J]. 分析化学, 2021, 49(2): 226-236. doi: 10.19756/j.issn.0253-3820.201438 shu
Citation:  LU Jia-Qi,  YE Tai,  XU Fei,  CAO Hui,  YUAN Min,  YU Jin-Song,  YIN Feng-Qin,  WU Xiu-Xiu. DNAzyme Dependent Self-Powered DNA Machine for Determination of Organophosphorus Pesticides[J]. Chinese Journal of Analytical Chemistry, 2021, 49(2): 226-236. doi: 10.19756/j.issn.0253-3820.201438 shu

基于DNAzyme的自驱动DNA分子机器检测有机磷农药

    通讯作者: 叶泰,E-mail:taiye@usst.edu.cn
  • 基金项目:

    国家自然科学基金项目(No.31801636)和上海市青年科技英才扬帆计划(No.18YF1417300)资助。

摘要: 通过范德华力作用,将脱氧核酶(DNAzyme)和6-羧基荧光素(FAM)标记的底物链共修饰的金纳米粒子(AuNPs)负载于MnO2纳米片上,构建了一种自驱动DNA分子机器。乙酰胆碱酯酶(AChE)将其底物乙酰硫代胆碱(ATCh)转化为硫代胆碱(TCh)后,MnO2纳米片被降解为Mn2+,激活DNAzyme切割底物链的循环,使FAM远离AuNPs表面,导致溶液荧光增强。当AChE被有机磷农药(OPs)抑制时,分子机器无法被激活。采用透射电镜(TEM)、紫外-可见吸收光谱、Zeta电位、动态光散射(DLS)和X射线光电子能谱(XPS)对所制备的DNA分子机器进行表征,并对其性能进行优化。在最优条件下,将该探针用于有机磷农药敌敌畏(DDVP)的检测,线性范围为5~500 μg/L,检出限为3.62 μg/L(3σ)。将此方法应用于生菜中DDVP的检测,加标回收率为96.5%~104.2%,相对标准偏差(RSD)为1.7%~7.5%。

English


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  • 收稿日期:  2020-07-27
  • 修回日期:  2020-12-30
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