基于DNAzyme的自驱动DNA分子机器检测有机磷农药

陆伽颀 叶泰 徐斐 曹慧 袁敏 于劲松 阴凤琴 吴秀秀

引用本文: 陆伽颀, 叶泰, 徐斐, 曹慧, 袁敏, 于劲松, 阴凤琴, 吴秀秀. 基于DNAzyme的自驱动DNA分子机器检测有机磷农药[J]. 分析化学, 2021, 49(2): 226-236. doi: 10.19756/j.issn.0253-3820.201438 shu
Citation:  LU Jia-Qi,  YE Tai,  XU Fei,  CAO Hui,  YUAN Min,  YU Jin-Song,  YIN Feng-Qin,  WU Xiu-Xiu. DNAzyme Dependent Self-Powered DNA Machine for Determination of Organophosphorus Pesticides[J]. Chinese Journal of Analytical Chemistry, 2021, 49(2): 226-236. doi: 10.19756/j.issn.0253-3820.201438 shu

基于DNAzyme的自驱动DNA分子机器检测有机磷农药

    通讯作者: 叶泰,E-mail:taiye@usst.edu.cn
  • 基金项目:

    国家自然科学基金项目(No.31801636)和上海市青年科技英才扬帆计划(No.18YF1417300)资助。

摘要: 通过范德华力作用,将脱氧核酶(DNAzyme)和6-羧基荧光素(FAM)标记的底物链共修饰的金纳米粒子(AuNPs)负载于MnO2纳米片上,构建了一种自驱动DNA分子机器。乙酰胆碱酯酶(AChE)将其底物乙酰硫代胆碱(ATCh)转化为硫代胆碱(TCh)后,MnO2纳米片被降解为Mn2+,激活DNAzyme切割底物链的循环,使FAM远离AuNPs表面,导致溶液荧光增强。当AChE被有机磷农药(OPs)抑制时,分子机器无法被激活。采用透射电镜(TEM)、紫外-可见吸收光谱、Zeta电位、动态光散射(DLS)和X射线光电子能谱(XPS)对所制备的DNA分子机器进行表征,并对其性能进行优化。在最优条件下,将该探针用于有机磷农药敌敌畏(DDVP)的检测,线性范围为5~500 μg/L,检出限为3.62 μg/L(3σ)。将此方法应用于生菜中DDVP的检测,加标回收率为96.5%~104.2%,相对标准偏差(RSD)为1.7%~7.5%。

English


    1. [1]

      KAUR J, SINGHP K. Phys. Chem. Chem. Phys., 2020, 22:15105-15119.

    2. [2]

      HASSANI S, MOMTAZ S, VAKHSHITEH F, MAGHSOUDI A S, GANJALI M R, NOROUZI P, ABDOLLAHI M. Arch. Toxicol., 2017, 91(1):109-130.

    3. [3]

      GB 2763-2019, National Food Safety Standard-Maximum Residue Limits for Pesticides in Food. National Standards of the People's Republic of China. 食品中农药最大残留限量.食品安全国家标准. 中华人民共和国国家标准. GB 2763-2019.

    4. [4]

      LI C, CHEN L, LI W. Microchim. Acta, 2013, 180:1109-1116.

    5. [5]

      XU Z, FANG G, WANG S. Food Chem., 2010, 119(2):845-850.

    6. [6]

      TIMOFEEVA I, SHISHOV A, KANASHINA D, DZEMA D, BULATOV A. Talanta, 2017, 167:761-767.

    7. [7]

      SOARES S, CASTRO T, ROSADO T, FERN NDEZ N, BARROSO M, GALLARDO E. Anal. Bioanal. Chem., 2018, 410(30):7955-7964.

    8. [8]

      WANG Fang-Huan, REN Cui-Juan, MA Hui, LI Ping, HAO Jun-Hu, CHEN Lin. Chin. J. Chromatogr., 2019, 37(10):1042-1047. 王芳焕, 任翠娟, 马辉, 李萍, 郝俊虎, 陈林. 色谱, 2019, 37(10):1042-1047.

    9. [9]

      HUANG Meng-Ying, WANG Na, GUO Xin-Yan, QIU Pan-Zi, TANG Wei-Guo, ZHANG Zun-Jian. Chin. J. Anal. Chem., 2016, 44(5):723-730. 黄梦莹, 王娜, 郭欣妍, 邱盼子, 汤卫国, 张尊建. 分析化学, 2016, 44(5):723-730.

    10. [10]

      LIU H, YU X, CHEN Y, ZHANG J, WU B, ZHENG L, HARUEHANROENGRA P, WANG R, LI S, LIN J, LI J, SHENG J, HUANG Z, MA J, GAN J. Nat. Commun., 2017, 8:2006.

    11. [11]

      MORRISON D, ROTHENBROKER M, LI Y. Small Methods, 2018, 2(3):1700319.

    12. [12]

      ZHANG H, TAO J, WANG Z, DENG W, NEWBIGGING A M, PENG H, LE X C. Anal. Chem., 2018, 90(1):190-207.

    13. [13]

      HE D, HE X, YANG X, LI H W. Chem. Sci., 2017, 8(4):2832-2840.

    14. [14]

      YIN Y, CHEN G, GONG L, GE K, PAN W, LI N, MACHUKI J O, YU Y, GENG D, DONG H, GAO F. Anal. Chem., 2020, 92(13):9247-9256.

    15. [15]

      WANG J, WANG H, WANG H, HE S, LI R, DENG Z, LIU X, WANG F. ACS Nano, 2019, 13(5):5852-5863.

    16. [16]

      LIU M, PENG Y, NIE Y, LIU P, HU S, DING J, ZHOU W. Acta Biomater., 2020, 110:242-253.

    17. [17]

      ZHENG L, ZHOU D, WU J, LIU C, CHEN J. Anal. Methods, 2019, 11(9):1168-1173.

    18. [18]

      WANG W, XU Y, CHEN N, XIE Y, HUANG K, XU W. Sens. Actuators B, 2020, 321:128598.

    19. [19]

      PAN J, LI F, CHA T G, CHEN H, CHOI J H. Curr. Opin. Biotechnol., 2015, 34:56-64.

    20. [20]

      PENG H, LI X F, ZHANG H, LE X C. Nat. Commun., 2017, 8:14378.

    21. [21]

      WANG J, WANG D X, TANG A N, KONG D M. Anal. Chem., 2019, 91(8):5244-5251.

    22. [22]

      CHEN J, ZUEHLKE A, DENG B, PENG H, HOU X, ZHANG H. Anal. Chem., 2017, 89(23):12888-12895.

    23. [23]

      DU H, YANG P, HOU X, HOU X, CHEN J. Microchem. J., 2018, 139:260-267.

    24. [24]

      DU H, YANG P, HOU X, ZHOU R, HOU X, CHEN J. Chem. Commun., 2019, 55(25):3610-3613.

    25. [25]

      XU M, ZHUANG J, JIANG X, LIU X, TANG D. Chem. Commun., 2019, 55(66):9857-9860.

    26. [26]

      YANG P, PENG X, YANG Z, CHAI Y, YUAN R, LIANG W. Chem. Eur. J., 2020, 26(40):8767-8773.

    27. [27]

      YANG Y, HUANG J, YANG X, HE X, QUAN K, XIE N, OU M, WANG K. Anal. Chem., 2017, 89(11):5850-5856.

    28. [28]

      TIAN F, ZHOU J, JIAO B, HE Y. Nanoscale, 2019, 11(19):9547-9555.

    29. [29]

      KAI K, YOUSHIDA Y, KAGEYAMA H, SAITO G, ISHIGAKI T, FURUKAWA Y, KAWAMATA J. J. Am. Chem. Soc., 2008, 130(47):15938-15943.

    30. [30]

      YE T, ZHANG Z, YUAN M, CAO H, YIN F, WU X, XU F. J. Agric. Food Chem., 2020, 68(9):2826-2831.

    31. [31]

      GB/T 5009.20-2003, Determination of Organophosphorus Pesticide Residues in Foods. National Standards of the People's Republic of China. 食品中有机磷农药残留量的测定. 中华人民共和国国家标准. GB/T 5009.20-2003.

    32. [32]

      CUI M R, LI X L, XU J J, CHEN H Y. Chem. Commun., 2019, 55(89):13366-13369.

    33. [33]

      CHEN J, MENG H, TIAN Y, YANG R, DU D, LI Z, QU L, LIN Y. Nanoscale Horiz., 2019, 4(2):434-444.

    34. [34]

      HE L, LU Y, WANG F, JING W, CHEN Y, LIU Y. Sens. Actuators B, 2018, 254:468-474.

    35. [35]

      FAN Wei, WANG Jing, WANG Ruo-Yan, CHEN Li. J. Hyg. Res., 2019, 48(2):259-264. 樊伟, 王晶, 王若燕, 陈理. 卫生研究, 2019, 48(2):259-264.

    36. [36]

      WANG L, HUANG Z, LIU Y, WU J, LIU J. Langmuir, 2018, 34(9):3094-3101.

    37. [37]

      United States Environmental Protcction Agency, Tolerances and Exemptions for Pesticide Chemical Residues in Food 40 CFR Part 180, 2014.

    38. [38]

      European Parliament and the Council, On Maximum Residue Levels of Pesticides in or on Food and Feed of Plant and Animal Origin and Amending Council Directive 91/414/EEC, 2005.

    39. [39]

      DOU X, CHU X, KONG W, LUO J, YANG M. Anal. Chim. Acta, 2015, 891:291-297.

    40. [40]

      CHEN Q, SHENG R, WANG P, OUYANG Q, WANG A, ALI S, ZAREEF M, HASSAN M M. Spectrochim. Acta, Part A, 2020, 241:118654.

    41. [41]

      LU Ting-Ting, WANG Jin-Long, ZHAN Xiang-Qiang, WU Yuan-Gen. Chin. J. Anal. Chem., 2020, 48(1):74-82. 陆婷婷, 王金龙, 詹相强, 吴远根. 分析化学, 2020, 48(1):74-82.

    42. [42]

      ZHANG C, WANG L, TU Z, SUN X, HE Q, LEI Z, XU C, LIU Y, ZHANG X, YANG J, LIU X, XU Y. Biosens. Bioelectron., 2014, 55:216-219.

    43. [43]

      LIU X, SONG M, HOU T, LI F. ACS Sens., 2017, 2(4):562-568.

    44. [44]

      LI X, TANG X, CHEN X, QU B, LU L. Talanta, 2018, 188:232-237.

    45. [45]

      HUANG N, QIN Y, LI M, CHEN T, LU M, ZHAO J. Analyst, 2019, 144(10):3436-3441.

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  • 收稿日期:  2020-07-27
  • 修回日期:  2020-12-30
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