基于同轴鞘流的毛细管电泳-电喷雾质谱接口的研究与应用

引用本文: 朱柳, 张乃倩, 方盼, 潘建章, 黄超兰, 方群. 基于同轴鞘流的毛细管电泳-电喷雾质谱接口的研究与应用[J]. 分析化学, 2021, 49(1): 34-41. doi: 10.19756/j.issn.0253-3820.201408 shu

Citation: ZHU Liu, ZHANG Nai-Qian, FANG Pan, PAN Jian-Zhang, WONG Catherine Chiu-Lan, FANG Qun. Development and Application of Capillary Electrophoresis-Electrospray Ionization Mass Spectrometry System Based on A Coaxial Sheath Flow Interface[J]. Chinese Journal of Analytical Chemistry, 2021, 49(1): 34-41. doi: 10.19756/j.issn.0253-3820.201408 shu

基于同轴鞘流的毛细管电泳-电喷雾质谱接口的研究与应用

朱柳 ^1, ,
张乃倩 ^1, ,
方盼 ^1, ,
潘建章 ^1, ,
黄超兰 ^{2,
,} ,
方群 ^{1,
,}

1.
浙江大学化学系微分析系统研究所, 杭州 310058;
2.
北京大学医学部精准医疗多组学研究中心, 北京 100191

通讯作者: 黄超兰,E-mail:catherine_wong@bjmu.edu.cn; 方群,E-mail:fangqun@zju.edu.cn

基金项目:
国家自然科学基金项目（Nos.21827806，21435004）资助。

摘要: 研制了一种基于双毛细管的伸出型同轴鞘流毛细管电泳-电喷雾电离质谱（CE-ESI-MS）接口。通过设计不同的电路连接方式，实现自制接口与两台具有不同结构ESI源的质谱仪器的在线联用。此CE-ESI-MS系统可利用毛细管尖端电喷雾产生的负压作用完成流体动力进样，可实现皮升级微量样品的引入。此接口制作简单，成本低廉，死体积小。基于优化的缓冲液组成、鞘液流速和CE分离毛细管的伸出长度，系统可在长时间运行中保持稳定的电喷雾，质谱信号的相对标准偏差（RSD）为3.4%。对利血平样品的连续9次进样的保留时间、半峰宽、峰高、峰面积的重现性分别为1.9%、9.2%、16.9%和8.2%。在22 cm的有效分离长度内，此系统可在9 min内完成18种标准氨基酸的分离分析；在9 min内实现5种标准多肽混合物的基线分离，分离效率可达到7.27×10⁵ plates/m；对于细胞色素C的酶解产物，可在8 min内分离鉴定18种肽段。

关键词:

English

Development and Application of Capillary Electrophoresis-Electrospray Ionization Mass Spectrometry System Based on A Coaxial Sheath Flow Interface

1.
Institute of Microanalytical Systems, Department of Chemistry, Zhejiang University, Hangzhou 310058, China;
2.
Center for Precision Medicine Multi-omics Research, Peking University Health Science Center, Beijing 100191, China

Corresponding author: WONG Catherine Chiu-Lan, ; FANG Qun,

Received Date: 13 July 2020
Revised Date: 31 August 2020
Fund Project:
Supported by the National Natural Science Foundation of China (Nos. 21827806, 21435004).

Abstract: A coaxial sheath-flow capillary electrophoresis-electrospray ionization mass spectrometry (CE-ESI-MS) interface based on dual capillaries was developed for performing rapid, efficient and sensitive analysis. By designing different electrode systems, the online combination between homemade interface and different ESI-MS systems was achieved. The CE-ESI-MS system utilized the suction effect to accomplish hydrodynamic injection without external force, and realized the introduction of picoliter samples. The interface had many advantages such as simple to build, low cost, good durability, minimal dead volume and low dilution effect based on optimized parameters. The spray stability was evaluated by continuous injection with a relative standard deviation (RSD) of 3.4%. Therefore, in the nine consecutive separations of reserpine, the RSD of retention time, full Width at Half Maximum and peak area were 1.9%, 9.2% and 8.2%, respectively. The present system was successfully applied to separate eighteen standard amino acids within 9 min. Moreover, the baseline separation of five standard peptides mixture was achieved within 9 min with the number of theoretical plates of 7.27×10⁵ plates/m. The present system also performed good performance in the analysis of cytochrome C trypsin digest.

Key words:

1. [1]
  STOLZ A, JOOSS K, HOCKER O, ROMER J, SCHLECHT J, NEUSUSS C. Electrophoresis, 2019, 40(1):79-112.
2. [2]
  TYCOVA A, LEDVINA V, KLEPARMIK K. Electrophoresis, 2017, 38(1):115-134.
3. [3]
  KLEPARMIK K. Electrophoresis, 2015, 36(1):159-178.
4. [4]
  BONVIN G, SCHAPPLER J, RUDAZ S. J. Chromatogr. A, 2012, 1267:17-31.
5. [5]
  KRENKOVA J, FORET F. Proteomics, 2012, 12(19-20):2978-2990.
6. [6]
  OLIVARES J A, NGUYEN N T, YONKER C R, SMITH R D. Anal. Chem., 1987, 59(8):1230-1232.
7. [7]
  HIRAYAMA A, ABE H, YAMAGUCHI N, TABATA S, TOMITA M, SOGA T. Electrophoresis, 2018, 39(11):1382-1389.
8. [8]
  JOHNSON R T, TO N H, STOBAUGH J F, LUNTE C E. Chromatographia, 2017, 80(7):1061-1067.
9. [9]
  MAXWELL E J, CHEN D D Y. Anal. Chim. Acta, 2008, 627(1):25-33.
10. [10]
  MOINI M. Anal. Chem., 2007, 79(11):4241-4246.
11. [11]
  WOJCIK R, DADA O O, SADILEK M, DOVICHI N J. Rapid Commun. Mass Spectrom., 2010, 24(17):2554-2560.
12. [12]
  SUN L, HEBERT A S, YAN X, ZHAO Y, WESTPHALL M S, RUSH M J P, ZHU G, CHAMPION M M, COON J J, DOVICHI N J. Angew. Chem. Int. Ed., 2015, 53(50):13931-13933.
13. [13]
  SUN L, ZHU G, ZHANG Z, MOU S, DOVICHI N J. J. Proteome Res., 2015, 14(5):2312-2321.
14. [14]
  MAXWELL E J, ZHONG X, CHEN D D Y. Anal. Chem., 2010, 82(20):8377-8381.
15. [15]
  MAXWELL E J, ZHONG X, ZHANG H, VAN ZEIJL N, CHEN D D Y. Electrophoresis, 2010, 31(7):1130-1137.
16. [16]
  GONZALEZ-RUIZ V, CODESIDO S, FAR J, RUDAZ S, SCHAPPLER J. Electrophoresis, 2016, 37(7-8):936-946.
17. [17]
  GONZALEZ-RUIZ V, CODESIDO S, RUDAZ S, SCHAPPLER J. Electrophoresis, 2018, 39(5-6):853-861.
18. [18]
  FANG P, PAN J Z, FANG Q. Talanta, 2018, 180:376-382.

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1.
沈阳化工大学材料科学与工程学院沈阳 110142

基于同轴鞘流的毛细管电泳-电喷雾质谱接口的研究与应用

通讯作者: 黄超兰,E-mail:catherine_wong@bjmu.edu.cn; 方群,E-mail:fangqun@zju.edu.cn

English

Development and Application of Capillary Electrophoresis-Electrospray Ionization Mass Spectrometry System Based on A Coaxial Sheath Flow Interface

Corresponding author: WONG Catherine Chiu-Lan, ; FANG Qun,

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

基于同轴鞘流的毛细管电泳-电喷雾质谱接口的研究与应用

通讯作者: 黄超兰,E-mail:catherine_wong@bjmu.edu.cn; 方群,E-mail:fangqun@zju.edu.cn

English

Development and Application of Capillary Electrophoresis-Electrospray Ionization Mass Spectrometry System Based on A Coaxial Sheath Flow Interface

Corresponding author: WONG Catherine Chiu-Lan, ; FANG Qun,

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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