基于碘刻蚀金纳米棒的比色法测定多巴胺

引用本文: 覃秀, 袁春玲, 石睿, 王述正, 王益林. 基于碘刻蚀金纳米棒的比色法测定多巴胺[J]. 分析化学, 2021, 49(1): 60-67. doi: 10.19756/j.issn.0253-3820.201384 shu

Citation: QIN Xiu, YUAN Chun-Ling, SHI Rui, WANG Shu-Zheng, WANG Yi-Lin. Colorimetric Detection of Dopamine Based on Iodine-mediated Etching of Gold Nanorods[J]. Chinese Journal of Analytical Chemistry, 2021, 49(1): 60-67. doi: 10.19756/j.issn.0253-3820.201384 shu

基于碘刻蚀金纳米棒的比色法测定多巴胺

广西大学化学化工学院, 广西生物炼制重点实验室, 南宁 530004

通讯作者: 王益林,E-mail:theanalyst@163.com

基金项目:
国家自然科学基金项目（No.61664002）、广西研究生教育创新计划项目（No.YCSW2020016）和广西大学大学生创新创业训练计划项目（No.202010593042）资助。

摘要: 基于金纳米棒（AuNRs）具有可调节的表面等离子共振（SPR）光学特性，以及多巴胺（DA）还原KIO₃生成的I₂刻蚀AuNRs，并使其光谱蓝移的原理，发展了一种以波长变化为响应信号的比色分析法，用于多巴胺（DA）的高灵敏测定。KIO₃不能刻蚀AuNRs，但在DA存在下，IO₃^-还原生成的I₂能刻蚀AuNRs，使其纵向局域表面等离子共振（LSPR）吸收峰蓝移，并伴有明显的由酒红色到蓝色的颜色变化。在保持AuNRs用量一定的条件下，考察了KIO₃浓度、温度、pH值和孵育时间等因素对信号响应的影响。结果表明，在KIO₃浓度为0.7 mmol/L、反应温度为50℃、pH=2.6及孵育时间为6 min的条件下，AuNRs吸收波长的蓝移值与DA浓度在0.80~60.0 μmol/L范围内呈良好的线性关系，方法的检出限为0.62 μmol/L（3σ/k），尿液中常见的共存物不干扰测定，实际尿液样品中DA的加标回收率在103.0%~110.8%之间。

关键词:

金纳米棒
/ 比色法
/ 多巴胺

English

Colorimetric Detection of Dopamine Based on Iodine-mediated Etching of Gold Nanorods

School of Chemistry and Chemical Engineering, Guangxi University, Guangxi Key Laboratory of Biorefinery, Nanning 530004, China

Corresponding author: WANG Yi-Lin, theanalyst@163.com

Received Date: 02 July 2020
Revised Date: 10 October 2020
Fund Project:
Supported by the National Natural Science Foundation of China (No.61664002), the Innovation Project of Guangxi Graduate Education (No.YCSW2020016) and the Foundation of College Student Innovation Ability Training of Guangxi University (No.202010593042).

Abstract: A colorimetric analytical method, with wavelength change as a response, was developed for the highly sensitive determination of dopamine (DA) based on gold nanorods (AuNRs) with tunable surface plasma-resonance optical properties and the fact that AuNRs could be etched by I₂ generated from KIO₃ reduction by DA. Even though AuNRs were unable to be etched by KIO₃, however, they surrendered to I₂ generated by IO₃^- in the presence of DA, which made their absorption peak of longitudinal localized surface plasma resonance (LSPR) a blue shift, accompanied by obvious color change from wine red to blue. The influence of KIO₃ concentration, temperature, pH value and incubation time on signal responses was investigated. The results showed that under the optimized conditions including 0.7 mmol/L KIO₃, reaction temperature at 50℃, pH 2.6 and incubation time of 6 min, the amount of blue shift of AuNRs absorption wavelength was linear with DA concentration in the range of 0.80-60.0 μmol/L. The detection limit was 0.62 μmol/L (3σ/k) and the matrix in urine did not interfere with the determination. The DA recovery in actual urine samples ranged from 103.0% to 110.8%.

Key words:

Gold nanorods
/ Colorimetric
/ Dopamine

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沈阳化工大学材料科学与工程学院沈阳 110142

基于碘刻蚀金纳米棒的比色法测定多巴胺

通讯作者: 王益林,E-mail:theanalyst@163.com

English

Colorimetric Detection of Dopamine Based on Iodine-mediated Etching of Gold Nanorods

Corresponding author: WANG Yi-Lin, theanalyst@163.com

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

基于碘刻蚀金纳米棒的比色法测定多巴胺

通讯作者: 王益林,E-mail:theanalyst@163.com

English

Colorimetric Detection of Dopamine Based on Iodine-mediated Etching of Gold Nanorods

Corresponding author: WANG Yi-Lin, theanalyst@163.com

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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CCS Chemistry：颜徐州、鲍哲南：你的皮肤可能是一片SPMs