分子印迹技术在蛋白质翻译后修饰分析中的应用

引用本文: 张攀, 姜丽艳, 马玖彤, 贾琼. 分子印迹技术在蛋白质翻译后修饰分析中的应用[J]. 分析化学, 2021, 49(1): 24-33. doi: 10.19756/j.issn.0253-3820.201369 shu

Citation: ZHANG Pan, JIANG Li-Yan, MA Jiu-Tong, JIA Qiong. Application of Molecular Imprinting Technology in Post-translational Modified Protein Enrichment[J]. Chinese Journal of Analytical Chemistry, 2021, 49(1): 24-33. doi: 10.19756/j.issn.0253-3820.201369 shu

分子印迹技术在蛋白质翻译后修饰分析中的应用

张攀 ^1, ,
姜丽艳 ^2, ,
马玖彤 ^{1,
,} ,
贾琼 ^{1,
,}

1.
吉林大学化学学院, 长春 130012;
2.
吉林大学生命科学学院, 长春 130012

通讯作者: 马玖彤,E-mail:majt@jlu.edu.cn; 贾琼,E-mail:jiaqiong@jlu.edu.cn

基金项目:
吉林省科技厅自然科学基金项目（No.20190201079JC）资助。

摘要: 蛋白质翻译后修饰在细胞的生命过程中发挥着重要的调控作用，可赋予蛋白质特定的生物活性，发挥相应的功能。翻译后修饰蛋白质在生物体中的含量非常低，因此，准确鉴别蛋白质的翻译后修饰面临着巨大的挑战。利用分子印迹技术制备的印迹材料，具有与目标分子尺寸、形状和功能基团匹配的印迹空腔，在翻译后修饰蛋白质的分离富集领域表现出了极大的应用潜力。本文概述了蛋白质翻译后修饰和分子印迹技术的种类、特点，在此基础上综述了分子印迹技术在糖基化、磷酸化、乙酰化、硫酸化翻译后修饰蛋白质富集中的应用，并对其发展前景进行了展望。

关键词:

English

Application of Molecular Imprinting Technology in Post-translational Modified Protein Enrichment

ZHANG Pan ^1, ,
JIANG Li-Yan ^2, ,
MA Jiu-Tong ^{1,
,} ,
JIA Qiong ^{1,
,}

1.
College of Chemistry, Jilin University, Changchun 130012, China;
2.
College of Life Sciences, Jilin University, Changchun 130012, China

Corresponding author: MA Jiu-Tong, ; JIA Qiong,

Received Date: 25 June 2020
Revised Date: 26 September 2020
Fund Project:
.

Abstract: Post-translational modification of proteins plays an important regulatory role in the process of cell life, enabling proteins to have biological activities and perform corresponding functions. The content of post-translational modification proteins in the organism is very low, which makes it commit to a mighty challenge to accurately identify the post-translational modification proteins. The molecularly imprinted cavity, which is perfectly matched with the size, shape and functional groups of the target molecules, has shown great potential in the separation of modified proteins. In this paper, the types and characteristics of post-translational modified proteins and molecular imprinting methods are summarized. On this basis, the application of molecularly imprinted polymers in the enrichment of post-translational modified proteins is reviewed, and the prospect of its development is prospected.

Key words:

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1.
沈阳化工大学材料科学与工程学院沈阳 110142

分子印迹技术在蛋白质翻译后修饰分析中的应用

通讯作者: 马玖彤,E-mail:majt@jlu.edu.cn; 贾琼,E-mail:jiaqiong@jlu.edu.cn

English

Application of Molecular Imprinting Technology in Post-translational Modified Protein Enrichment

Corresponding author: MA Jiu-Tong, ; JIA Qiong,

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

分子印迹技术在蛋白质翻译后修饰分析中的应用

通讯作者: 马玖彤,E-mail:majt@jlu.edu.cn; 贾琼,E-mail:jiaqiong@jlu.edu.cn

English

Application of Molecular Imprinting Technology in Post-translational Modified Protein Enrichment

Corresponding author: MA Jiu-Tong, ; JIA Qiong,

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

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CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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