耗散型石英晶体微天平实时监测氧化损伤红细胞与内皮细胞的粘附

引用本文: 肖苏婷, 姚政, 周琳, 杨培慧. 耗散型石英晶体微天平实时监测氧化损伤红细胞与内皮细胞的粘附[J]. 分析化学, 2020, 48(11): 1550-1555. doi: 10.19756/j.issn.0253-3820.201335 shu

Citation: XIAO Su-Ting, YAO Zheng, ZHOU Lin, YANG Pei-Hui. Real-time Monitoring of Adhesion between Oxidative Damaged-Erythrocytes and Endothelial Cells Based on Quartz Crystal Microbalance[J]. Chinese Journal of Analytical Chemistry, 2020, 48(11): 1550-1555. doi: 10.19756/j.issn.0253-3820.201335 shu

耗散型石英晶体微天平实时监测氧化损伤红细胞与内皮细胞的粘附

暨南大学化学与材料学院, 广州 510632

通讯作者: 杨培慧,typh@jnu.edu.cn

基金项目:
本文系国家自然科学基金项目（No.21874057）资助

摘要: 红细胞与血管内皮细胞之间的粘附作用研究对揭示心血管疾病的发病机制具有重要意义。在病理状态下，红细胞的氧化损伤会使胞膜糖蛋白受体中的唾液酸含量下调以及细胞粘弹性质的改变，导致红细胞与血管内皮细胞的黏附性增强。基于唾液酸与3-氨基苯硼酸具有特异性识别作用，本研究采用耗散型石英晶体微天平（QCM-D）建立了一种实时监测氧化损伤红细胞与内皮细胞之间粘附的新方法。以H₂O₂氧化损伤红细胞为研究模型，通过QCM传感器实时监测红细胞粘附过程频率和耗散因子的变化，评价其氧化损伤程度。结果表明，随着H₂O₂浓度增加，QCM频率和耗散因子变化值均随之减小，表明红细胞的粘附与H₂O₂浓度和红细胞的氧化损伤程度呈负相关。为了模拟血管内环境，将血管内皮细胞固定于芯片界面，采用QCM实时监测其与红细胞的粘附，结果表明，随着红细胞损伤程度增加，细胞间的黏附逐渐增强。本研究建立了一种简便、免标记、高灵敏、可实时监测细胞间粘附并评价细胞氧化损伤的新方法，拓展了QCM技术在生物体系细胞功能研究中的应用范围。

关键词:

English

Real-time Monitoring of Adhesion between Oxidative Damaged-Erythrocytes and Endothelial Cells Based on Quartz Crystal Microbalance

College of Chemistry and Materials Science, Jinan University, Guangzhou 510632, China

Corresponding author: YANG Pei-Hui, typh@jnu.edu.cn

Received Date: 10 June 2020
Revised Date: 06 August 2020
Fund Project:
This work was supported by the National Natural Science Foundation of China (No. 21874057).

Abstract: The investigation of the adhesion between red blood cells and vascular endothelial cells is of great significance to reveal the pathogenesis of cardiovascular disease. Under pathological conditions, the oxidative damage of red blood cells (RBCs) can cause down-regulating content of sialic acid combined with glycoprotein receptors on the cell membrane, and change the viscoelastic properties of the cells, lead to the enhancement of the adhesion of erythrocytes to vascular endothelial cells. In this work, a new method based on specific recognition of sialic acid and 3-aminophenylboronic acid integration with quartz crystal microbalance with dissipation (QCM-D) was developed for real-time monitoring the adhesion between damaged-erythrocytes and endothelial cells. Taking H₂O₂ oxidative damage to erythrocytes as a research model, the changes of the frequency and dissipation factor during cell adhesion process were monitored by QCM sensor, which was used to evaluate the degree of erythrocytes oxidative damage. The results showed that both the QCM frequency change and the dissipation factor decreased accordingly with the increase of H₂O₂ concentration. It revealed that the adhesion of erythrocytes was negatively correlated with the concentration of H₂O₂ and the degree of RBCs damage. Furthermore, endothelial cells were fixed on the sensing interface for simulating intravascular environment and their adhesion to red blood cells was monitored by QCM in real time. The results showed that the adhesion between cells increased with the increase of the degree of erythrocytes damage. Therefore, the strategy provided a simple, label free, highly sensitive and real-time method for the monitoring of cell adhesion and the evaluation of cells oxidative damage, which expended the application of QCM in the research of cell function in biological system.

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沈阳化工大学材料科学与工程学院沈阳 110142

耗散型石英晶体微天平实时监测氧化损伤红细胞与内皮细胞的粘附

通讯作者: 杨培慧,typh@jnu.edu.cn

English

Real-time Monitoring of Adhesion between Oxidative Damaged-Erythrocytes and Endothelial Cells Based on Quartz Crystal Microbalance

Corresponding author: YANG Pei-Hui, typh@jnu.edu.cn

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

耗散型石英晶体微天平实时监测氧化损伤红细胞与内皮细胞的粘附

通讯作者: 杨培慧,typh@jnu.edu.cn

English

Real-time Monitoring of Adhesion between Oxidative Damaged-Erythrocytes and Endothelial Cells Based on Quartz Crystal Microbalance

Corresponding author: YANG Pei-Hui, typh@jnu.edu.cn

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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