基于聚集诱导发光分子的免标记癌胚抗原生物传感新方法研究

引用本文: 李海银, 常加富, 吕文欣, 李峰. 基于聚集诱导发光分子的免标记癌胚抗原生物传感新方法研究[J]. 分析化学, 2020, 48(10): 1325-1333. doi: 10.19756/j.issn.0253-3820.201295 shu

Citation: LI Hai-Yin, CHANG Jia-Fu, LYU Wen-Xin, LI Feng. Aggregation Induced Emission Fluorogen-based Label-free Biosensor for Highly Sensitive Detection of Carcinoembryonic Antigen[J]. Chinese Journal of Analytical Chemistry, 2020, 48(10): 1325-1333. doi: 10.19756/j.issn.0253-3820.201295 shu

基于聚集诱导发光分子的免标记癌胚抗原生物传感新方法研究

青岛农业大学化学与药学院, 青岛 266109
基金项目:
本文系国家自然科学基金项目（Nos.21605093，21775082）资助

摘要: 基于目标物诱导酶循环放大反应，借助Hemin/G-四链体对L-半胱氨酸（L-Cys）的催化氧化作用，构建了聚集诱导发光（AIE）分子介导的荧光生物传感器，实现了癌胚抗原（CEA）的免标记、高灵敏检测。以弱发光的马来酰亚胺功能化四苯乙烯（TPE-M）作为信号源，其可与L-Cys反应，使荧光增强。当目标物存在时，CEA引发聚合酶/内切酶辅助的循环放大反应，原位生成大量Hemin/G-四链体，其催化氧化L-Cys变成胱氨酸（Cys-cys），阻止L-Cys与TPE-M反应，致使传感体系的荧光强度降低；当CEA不存在时，L-Cys可继续与TPE-M反应，体系荧光信号增强。基于体系中荧光信号的变化，即可实现CEA的免标记、高灵敏检测，检出限为0.033 fmol/L。本传感器具有优异的选择性、稳定性与抗干扰能力，为生物样品中CEA的灵敏与准确检测提供了新方法。

关键词:

English

Aggregation Induced Emission Fluorogen-based Label-free Biosensor for Highly Sensitive Detection of Carcinoembryonic Antigen

College of Chemistry and Pharmaceutical Sciences, Qingdao Agricultural University, Qingdao 266109, China
Received Date: 22 May 2020
Revised Date: 02 July 2020
Fund Project:
This work was supported by the National Natural Science Foundation of China (Nos. 21605093, 21775082).

Abstract: A highly sensitive and label-free fluorescence carcinoembryonic antigen (CEA) biosensor was developed via target-triggered enzymatic recycling amplification reaction. In this strategy, aggregation induced emission fluorogen (AIEgen) TPE-M was chosen as a signal reporter to enhance the detection efficiency, as well as L-cysteine (L-cys) was utilized as a reactant to control over the fluorescence intensity due to its distinct capability of reacting with TPE-M and hemin/G-quadruplex. CEA triggered the in-situ generation of abundant hemin/G-quadruplex through polymerase/nicking enzyme-assisted recycling amplification, and the in-situ generated hemin/G-quadruplex catalyzed the destruction of L-cys. With L-cys consuming, a significantly decreased fluorescence was observed, demonstrating that the fluorescence intensity was relied on CEA amount. As a consequence, a facile, precise and sensitive strategy for CEA assay was readily realized. Furthermore, the detection limit was 0.033 fmol/L (S/N=3). The developed AIEgen-based biosensor provided a new method for sensitive and reliable detection of CEA in biological liquids, displaying a significant promise for CEA-related disease diagnosis.

Key words:

1. [1]
  Qiu Z, Shu J, Liu J, Tang D. Anal. Chem., 2019, 91(2):1260-1268
2. [2]
  HAN Yu-Ping, CHEN Lin, LI Zhen, HU Cheng-Guo, LIU Zhi-Hong. Chinese J. Anal. Chem., 2018, 46(8):1178-1185 韩玉平, 谌林, 李贞, 胡成国, 刘志洪. 分析化学, 2018, 46(8):1178-1185
3. [3]
  Su S, Li J, Yao Y, Sun Q, Zhao Q, Wang F, Li Q, Liu X G, Wang L H. ACS Appl. Bio. Mater., 2019, 2(1):292-298
4. [4]
  Xiao L P, Zhu A M, Xu Q C, Chen Y, Xu J, Weng J. ACS Appl. Mater. Interfaces, 2017, 9(8):6931-6940
5. [5]
  Liu F R, Cao J T, Wang Y L, Fu X L, Ren S W, Liu Y M. Sens. Actuators B, 2018, 276(10):173-179
6. [6]
  Yang N, Huang Y X, Ding G S, Fan A P. Anal. Chem., 2019, 91(7):4906-4912
7. [7]
  Zhao L J, Cheng M, Liu G N, Lu H Y, Gao Y, Yan X, Liu F M, Sun P, Lu G Y. Sens. Actuators B, 2018, 273(10):185-190
8. [8]
  Jie G F, Li C L, Zhao Y, Kuang Q, Niu S Y. ACS Appl. Nano Mater., 2019, 2(7):4637-4645
9. [9]
  Jia Y L, Li Y Y, Zhang S A, Wang P, Liu Q, Dong Y H. Biosens. Bioelectron., 2020, 149(1):111842
10. [10]
  Ge L, Wang W X, Sun X M, Hou T, Li F. Anal. Chem., 2016, 88(4):2212-2219
11. [11]
  Lin X L, Wang Y Y, Wang L N, Lu Y D, Li J, Lu D C, Zhou T, Huang Z F, Huang J, Huang H F, Qiu S F, Chen R, Lin D, Feng S Y. Biosens. Bioelectron., 2019, 143(15):111599
12. [12]
  Bai X R, Wang L H, Ren J Q, Bai X W, Zeng L W, Shen A G, Hu J M. Anal. Chem., 2019, 91(4):2955-2963
13. [13]
  Yang X, Zhuo Y, Zhu S, Luo Y, Feng Y, Xu Y. Biosens. Bioelectron., 2015, 64:345-351
14. [14]
  Hai X M, Li N, Wang K, Zhang Z Q, Zhang J, Dang F Q. Anal. Chim. Acta, 2018, 998:60-66
15. [15]
  Qian R C, Cao Y, Zhao L J, Gu Z, Long Y T. Angew. Chem. Int. Ed., 2017, 56(17):4802-4805
16. [16]
  Liu Q Y, Chen P P, Xu Z, Chen M M, Ding Y N, Yue K, Xu J. Sens. Actuators B, 2017, 251:339-348
17. [17]
  Tzouvadaki I, Jolly P, Lu X L, Ingebrandt S, de Micheli G, Estrela P, Carrara S. Nano Lett., 2016, 16(7):4472-4476
18. [18]
  Jie G, Li C, Zhao Y, Kuang Q, Niu S. ACS Appl. Nano Mater., 2019, 2(7):4637-4645
19. [19]
  Miao H, Wang L, Zhuo Y, Zhou Z, Yang X. Biosens. Bioelectron., 2016, 86:83-89
20. [20]
  Huang W, Hu G B, Yao L Y, Yang Y, Liang W B, Yuan R, Xiao D R. Anal. Chem., 2020, 92(4):3380-3387
21. [21]
  Li J, Kwon N, Jeong Y, Lee S, Kim G, Yoon J. ACS Appl. Mater. Interfaces, 2018, 10(15):12150-12154
22. [22]
  Wu P, Wang X F, Wang Z G, Ma W, Guo J S, Chen J J, Yu Z Q, Li J Z, Zhou D F. ACS Appl. Mater. Interfaces, 2019, 11(20):18691-18700
23. [23]
  Li H Y, Lin H Y, Lv W X, Gai P P, Li F. Biosens. Bioelectron., 2020, 165:112336
24. [24]
  Lu D, He L, Wang Y, Xiong M, Hu M, Liang H, Huan S, Zhang X B, Tan W. Talanta, 2017, 167:550-556
25. [25]
  Zhuang Y, Huang F J, Xu Q, Zhang M S, Lou X D, Xia F. Anal. Chem., 2016, 88(6):3289-3294
26. [26]
  Chang J F, Li H Y, Hou T, Duan W N, Li F. Biosens. Bioelectron., 2018, 104(1):152-157
27. [27]
  Chang J F, Li H Y, Hou T, Li F. Biosens. Bioelectron., 2016, 86(15):971-977
28. [28]
  Li H Y, Chang J F, Gai P P, Li F. ACS Appl. Mater. Interfaces, 2018, 10(5):4561-4568
29. [29]
  Zhou Y C, Ran X X, Chen A Y, Chai Y Q, Yuan R, Zhuo Y. Anal. Chem., 2018, 90(15):9109-9116
30. [30]
  Shen P, Li W, Liu Y, Ding Z, Deng Y, Zhu X, Jin Y, Li Y, Li J, Zheng T. Anal. Chem., 2017, 89(21):11862-11868
31. [31]
  Ren K W, Wu J, Ju H X, Yan F. Anal. Chem., 2015, 87(3):1694-1700
32. [32]
  Zhou J, Lai W Q, Zhuang J Y, Tang J, Tang D P. ACS Appl. Mater. Interfaces, 2013, 5(7):2773-2781
33. [33]
  Zong C, Wu J, Liu M M, Yang L L, Yan F, Ju H X. Anal. Chem., 2014, 86(19):9939-9944
34. [34]
  Xu J, Wu J, Zong C, Ju H X, Yan F. Anal. Chem., 2013, 85(6):3374-3379
35. [35]
  Lin Z Y, Zhang G Y, Yang W Q, Qiu B, Chen G N. Chem. Commun., 2012, 48(79):9918-9920
36. [36]
  Hu M, He Y, Song S P, Yan J, Lu H T, Weng L X, Wang L H, Fan C H. Chem. Commun., 2010, 46(33):6126-6128

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沈阳化工大学材料科学与工程学院沈阳 110142

基于聚集诱导发光分子的免标记癌胚抗原生物传感新方法研究

English

Aggregation Induced Emission Fluorogen-based Label-free Biosensor for Highly Sensitive Detection of Carcinoembryonic Antigen

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

基于聚集诱导发光分子的免标记癌胚抗原生物传感新方法研究

English

Aggregation Induced Emission Fluorogen-based Label-free Biosensor for Highly Sensitive Detection of Carcinoembryonic Antigen

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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