基于聚集诱导发光分子的免标记癌胚抗原生物传感新方法研究

引用本文: 李海银, 常加富, 吕文欣, 李峰. 基于聚集诱导发光分子的免标记癌胚抗原生物传感新方法研究[J]. 分析化学, 2020, 48(10): 1325-1333. doi: 10.19756/j.issn.0253-3820.201295 shu

Citation: LI Hai-Yin, CHANG Jia-Fu, LYU Wen-Xin, LI Feng. Aggregation Induced Emission Fluorogen-based Label-free Biosensor for Highly Sensitive Detection of Carcinoembryonic Antigen[J]. Chinese Journal of Analytical Chemistry, 2020, 48(10): 1325-1333. doi: 10.19756/j.issn.0253-3820.201295 shu

基于聚集诱导发光分子的免标记癌胚抗原生物传感新方法研究

青岛农业大学化学与药学院, 青岛 266109
基金项目:
本文系国家自然科学基金项目（Nos.21605093，21775082）资助

摘要: 基于目标物诱导酶循环放大反应，借助Hemin/G-四链体对L-半胱氨酸（L-Cys）的催化氧化作用，构建了聚集诱导发光（AIE）分子介导的荧光生物传感器，实现了癌胚抗原（CEA）的免标记、高灵敏检测。以弱发光的马来酰亚胺功能化四苯乙烯（TPE-M）作为信号源，其可与L-Cys反应，使荧光增强。当目标物存在时，CEA引发聚合酶/内切酶辅助的循环放大反应，原位生成大量Hemin/G-四链体，其催化氧化L-Cys变成胱氨酸（Cys-cys），阻止L-Cys与TPE-M反应，致使传感体系的荧光强度降低；当CEA不存在时，L-Cys可继续与TPE-M反应，体系荧光信号增强。基于体系中荧光信号的变化，即可实现CEA的免标记、高灵敏检测，检出限为0.033 fmol/L。本传感器具有优异的选择性、稳定性与抗干扰能力，为生物样品中CEA的灵敏与准确检测提供了新方法。

关键词:

English

Aggregation Induced Emission Fluorogen-based Label-free Biosensor for Highly Sensitive Detection of Carcinoembryonic Antigen

College of Chemistry and Pharmaceutical Sciences, Qingdao Agricultural University, Qingdao 266109, China
Received Date: 22 May 2020
Revised Date: 02 July 2020
Fund Project:
This work was supported by the National Natural Science Foundation of China (Nos. 21605093, 21775082).

Abstract: A highly sensitive and label-free fluorescence carcinoembryonic antigen (CEA) biosensor was developed via target-triggered enzymatic recycling amplification reaction. In this strategy, aggregation induced emission fluorogen (AIEgen) TPE-M was chosen as a signal reporter to enhance the detection efficiency, as well as L-cysteine (L-cys) was utilized as a reactant to control over the fluorescence intensity due to its distinct capability of reacting with TPE-M and hemin/G-quadruplex. CEA triggered the in-situ generation of abundant hemin/G-quadruplex through polymerase/nicking enzyme-assisted recycling amplification, and the in-situ generated hemin/G-quadruplex catalyzed the destruction of L-cys. With L-cys consuming, a significantly decreased fluorescence was observed, demonstrating that the fluorescence intensity was relied on CEA amount. As a consequence, a facile, precise and sensitive strategy for CEA assay was readily realized. Furthermore, the detection limit was 0.033 fmol/L (S/N=3). The developed AIEgen-based biosensor provided a new method for sensitive and reliable detection of CEA in biological liquids, displaying a significant promise for CEA-related disease diagnosis.

Key words:

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沈阳化工大学材料科学与工程学院沈阳 110142

基于聚集诱导发光分子的免标记癌胚抗原生物传感新方法研究

English

Aggregation Induced Emission Fluorogen-based Label-free Biosensor for Highly Sensitive Detection of Carcinoembryonic Antigen

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

基于聚集诱导发光分子的免标记癌胚抗原生物传感新方法研究

English

Aggregation Induced Emission Fluorogen-based Label-free Biosensor for Highly Sensitive Detection of Carcinoembryonic Antigen

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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