核酸适配体在质谱中的应用与展望

引用本文: 杨捷威, 王晨钰, 罗黎, 郭磊, 谢剑炜. 核酸适配体在质谱中的应用与展望[J]. 分析化学, 2020, 48(11): 1439-1447. doi: 10.19756/j.issn.0253-3820.201285 shu

Citation: YANG Jie-Wei, WANG Chen-Yu, LUO Li, GUO Lei, XIE Jian-Wei. Applications and Prospects of Oligonucleotide Aptamers in Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, 2020, 48(11): 1439-1447. doi: 10.19756/j.issn.0253-3820.201285 shu

核酸适配体在质谱中的应用与展望

杨捷威 ^1, ,
王晨钰 ^1,2, ,
罗黎 ^1,3, ,
郭磊 ^{1,
,} ,
谢剑炜 ^1,

1.
军事科学院军事医学研究院毒物药物研究所, 抗毒药物与毒理学国家重点实验室, 北京 100850;
2.
中央民族大学, 药学院, 北京 100081;
3.
河北医科大学, 公共卫生学院, 石家庄 050017

通讯作者: 郭磊,guolei@bmi.ac.cn

基金项目:
本文系国家自然科学基金项目（No.21974152）资助

摘要: 核酸适配体（简称适配体）是一类重要的"化学抗体"型功能性生物分子，基于适配体的质谱技术在提供分子质量及结构特征等基础上，兼具了针对靶分子的高选择性及亲和富集特点，从而提供出高特异、高灵敏信息。本文综述了近年来适配体在质谱分析中的应用进展，重点评述了适配体在质谱分子相互作用表征中的应用、适配体作为离线型和在线亲和材料用于质谱分析测定等现状，其中结合多种质谱新技术，通过创建或结合多种前处理或在线一体化信号增强方式，特别是适配体功能化纳米材料的应用，在相互作用表征、痕量靶分子选择性提取和高灵敏检测等质谱分析测定方面是研究的重点。最后，本文对适配体在质谱研究中的应用前景进行了展望。

关键词:

English

Applications and Prospects of Oligonucleotide Aptamers in Mass Spectrometry

1.
State Key Laboratory of Toxicology and Medical Countermeasures, Institute of Toxicology and Pharmacology, Academy of Military Medical Sciences, Beijing 100850, China;
2.
College of Pharmacy, Minzu University of China, Beijing 100081, China;
3.
School of Public Health, Hebei Medical University, Shijiazhuang 050017, China

Corresponding author: GUO Lei, guolei@bmi.ac.cn

Received Date: 19 May 2020
Revised Date: 19 August 2020
Fund Project:
This work was supported by the National Natural Science Foundation of China (No.21974152).

Abstract: Oligonucleotide aptamers are an important kind of chemical antibody-like functional biomolecules. The aptamer-based mass spectrometric techniques can not only provide molecular mass and characteristic structures by mass spectrometry, but show high sensitivity and high affinity enrichment towards target molecules by aptamers. This review summarized the advances in the aptamer-based mass spectrometric applications, highlighted the progress of aptamer-target interaction characterization, as well as the applications of aptamer as off-line or on-line affinity materials in mass spectrometric techniques. It suggested that the combination of various new mass spectrometric techniques, the construction or combination of varied pretreatment or on-line signal amplification approaches, especially the applications of aptameric functional nanomaterials, should be focused in the aspects of interaction characterization, selective extraction and highly sensitive detection of trace target molecules. The future prospects of the development were also described.

Key words:

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1.
沈阳化工大学材料科学与工程学院沈阳 110142

核酸适配体在质谱中的应用与展望

通讯作者: 郭磊,guolei@bmi.ac.cn

English

Applications and Prospects of Oligonucleotide Aptamers in Mass Spectrometry

Corresponding author: GUO Lei, guolei@bmi.ac.cn

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

核酸适配体在质谱中的应用与展望

通讯作者: 郭磊,guolei@bmi.ac.cn

English

Applications and Prospects of Oligonucleotide Aptamers in Mass Spectrometry

Corresponding author: GUO Lei, guolei@bmi.ac.cn

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

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CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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