膜萃取电喷雾电离质谱快速分析菌血血样的研究

引用本文: 王姜, 黄丽娟, 徐贞俊, 刘昱, 徐静娟, 陈洪渊. 膜萃取电喷雾电离质谱快速分析菌血血样的研究[J]. 分析化学, 2020, 48(10): 1315-1324. doi: 10.19756/j.issn.0253-3820.201263 shu

Citation: WANG Jiang, HUANG Li-Juan, XU Zhen-Jun, LIU Yu, XU Jing-Juan, CHEN Hong-Yuan. Rapid Analysis of Bacteremia by Membrane Extraction Electrospray Ionization Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, 2020, 48(10): 1315-1324. doi: 10.19756/j.issn.0253-3820.201263 shu

膜萃取电喷雾电离质谱快速分析菌血血样的研究

王姜 ^1, ,
黄丽娟 ^1, ,
徐贞俊 ^2, ,
刘昱 ^1, ,
徐静娟 ^1, ,
陈洪渊 ^1,

1.
南京大学生命分析化学国家重点实验室, 南京大学化学化工学院, 南京 210023;
2.
南京大学医学院附属鼓楼医院心胸外科, 南京 210008
基金项目:
本文系国家自然科学基金项目（Nos.21327902，21974060）资助

摘要: 采用自行研制的膜萃取电喷雾电离质谱（MEESI-MS），将大肠杆菌、金黄色葡萄球菌、肺炎克雷伯氏菌和铜绿假单胞菌4种细菌通过血液培养后，正、负离子模式下进行质谱检测，获得其化学指纹图谱，通过主成分分析（PCA）方法对所获指纹谱图信息进行分析，并对样品进行分类。结果表明，MEESI-MS可快速获取菌血血样化学指纹谱图，结合PCA方法对菌血血样中细菌种类进行快速鉴别，菌血样品培养时间约60 min，整体分析时间少于65 min。本方法样品预处理简单、操作简便、分析速度快，可望用于临床菌血症和败血症的细菌种类的快速鉴别。

关键词:

English

Rapid Analysis of Bacteremia by Membrane Extraction Electrospray Ionization Mass Spectrometry

1.
State Key Laboratory of Analytical Chemistry for Life Science and Collaborative Innovation Center of Chemistry for Life Sciences, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China;
2.
Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School Cardiothoracic Surgery, Nanjing 210008, China
Received Date: 08 May 2020
Revised Date: 24 June 2020
Fund Project:
This work was supported by the National Natural Science Foundation of China (Nos.21327902, 21974060).

Abstract: A custom membrane extraction electrospray ionization mass spectrometry (MEESI-MS) method was developed to detect the representative bacteric metabolites and classify four kinds of bacteria including Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae and Pseudomonas aeruginosa. The chemical fingerprints of each bacterium were obtained using this MEESI-MS method. Principal component analysis (PCA) was then applied to analyze the information from the obtained fingerprint spectra and classify the samples. The results showed that MEESI-MS method was capable of rapidly obtaining metabolic fingerprints of blood samples and direct identifying the representative metabolites from each bacterium via employing both accurate mass measurements and structural information from tandem mass spectra. The overall analysis time was less than 65 min (including a culture time of about 60 min). This method was featured by the simplicity in sample pretreatment, ionization activation and data analysis and was promising in rapid identification of bacterial species in clinical bacteremia and septicemia.

Key words:

Membrane extraction electrospray ionization
/ Mass spectrometry
/ Principal component analysis
/ Bacteremia
/ Bloodstream infection

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沈阳化工大学材料科学与工程学院沈阳 110142

膜萃取电喷雾电离质谱快速分析菌血血样的研究

English

Rapid Analysis of Bacteremia by Membrane Extraction Electrospray Ionization Mass Spectrometry

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

膜萃取电喷雾电离质谱快速分析菌血血样的研究

English

Rapid Analysis of Bacteremia by Membrane Extraction Electrospray Ionization Mass Spectrometry

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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