一步法合成聚乙烯亚胺保护的荧光银纳米簇用于甲硝唑检测

引用本文: 刘梦旋, 尹建行, 孟磊, 徐娜. 一步法合成聚乙烯亚胺保护的荧光银纳米簇用于甲硝唑检测[J]. 分析化学, 2020, 48(11): 1556-1563. doi: 10.19756/j.issn.0253-3820.201257 shu

Citation: LIU Meng-Xuan, YIN Jian-Hang, MENG Lei, XU Na. One-step Synthesis of Polyethylenimine-Protected Fluorescent Silver Nanoclusters and Its Application in Detection of Metronidazole[J]. Chinese Journal of Analytical Chemistry, 2020, 48(11): 1556-1563. doi: 10.19756/j.issn.0253-3820.201257 shu

一步法合成聚乙烯亚胺保护的荧光银纳米簇用于甲硝唑检测

刘梦旋 ^1, ,
尹建行 ^2, ,
孟磊 ^1, ,
徐娜 ^{1,
,}

1.
吉林化工学院材料科学与工程学院, 吉林 132022;
2.
吉林化工学院化学与制药工程学院, 吉林 132022

通讯作者: 徐娜,xn_1216@163.com

基金项目:
本文系吉林省科技厅科研项目（No.20180520006JH）和吉林省教育厅科研项目（No.JJKH20200241JK）资助

摘要: 甲硝唑（MNZ）是普遍使用的一种硝基咪唑类抗生素，多用于治疗厌氧菌引起的感染或动物饲料的添加剂，残留过量时会产生致畸、致癌等作用，因此准确检测MNZ十分必要。本研究利用微波技术，采用一步法成功合成了聚乙烯亚胺保护的银纳米簇（AgNCs@PEI）荧光探针，所合成的AgNCs在高离子强度及长时间光照下具有良好的荧光稳定性。AgNCs配体中的大量氨基官能团与MNZ中咪唑环内N原子之间的氢键作用，使二者形成稳定的非辐射基态配合物，导致AgNCs荧光强度下降，基于此建立了检测MNZ的荧光分析方法。线性范围为0.1-200 μmol/L，检出限为0.038 μmol/L（S/N=3），方法具有良好的选择性。将此荧光探针应用于人尿液中MNZ的测定，回收率为95.8%-103.4%，相对标准偏差小于4.3%。

关键词:

English

One-step Synthesis of Polyethylenimine-Protected Fluorescent Silver Nanoclusters and Its Application in Detection of Metronidazole

1.
College of Materials Science and Engineering, Jilin Institute of Chemical Technology, Jilin 132022, China;
2.
College of Chemistry and Pharmaceutical Engineering, Jilin Institute of Chemical Technology, Jilin 132022, China

Corresponding author: XU Na, xn_1216@163.com

Received Date: 06 May 2020
Revised Date: 28 August 2020
Fund Project:
This work was supported by the Project of Science and Technology Department of Jilin Province (No. 20180520006JH) and the Project of Education Department of Jilin Province, China (No. JJKH20200241JK).

Abstract: Metronidazole (MNZ) of nitroimidazole antibiotics is a kind of antibiotic commonly used in many fields. It is mostly used to treat the infection caused by anaerobic bacteria or as an additive in animal feed. However, when MNZ remains in the human body in excess, it will lead to teratogenic, carcinogenic and other serious side effects. In this work, a novel fluorescent probe based on polyethylenimine stabilized silver nanoclusters (AgNCs@PEI) was successfully synthesized by one-step microwave method. The as-prepared AgNCs@PEI showed good fluorescence stability in a wide ion concentration range or after long time of illumination (at 455 nm for 30 min). The hydrogen bonds between amino functional groups of PEI ligand and N atoms of the imidazole ring of MNZ resulted in formation of a stable non-radiative ground state complex that caused the decrease of fluorescence intensity of the AgNCs. The results showed that the probe had good selectivity for MNZ, with a linear range of 0.1-200 mol/L and a detection limit of 0.038 mol/L. The fluorescent probe was applied to determination of MNZ in human urine samples with recoveries of 95.8%-103.4%, and the relative standard deviations were less than 4.3%, indicating the new fluorescence probe had potential application value in drug detection.

Key words:

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沈阳化工大学材料科学与工程学院沈阳 110142

一步法合成聚乙烯亚胺保护的荧光银纳米簇用于甲硝唑检测

通讯作者: 徐娜,xn_1216@163.com

English

One-step Synthesis of Polyethylenimine-Protected Fluorescent Silver Nanoclusters and Its Application in Detection of Metronidazole

Corresponding author: XU Na, xn_1216@163.com

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

一步法合成聚乙烯亚胺保护的荧光银纳米簇用于甲硝唑检测

通讯作者: 徐娜,xn_1216@163.com

English

One-step Synthesis of Polyethylenimine-Protected Fluorescent Silver Nanoclusters and Its Application in Detection of Metronidazole

Corresponding author: XU Na, xn_1216@163.com

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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