基于DNA酶/AuNPs-GO传感器方波伏安法高灵敏检测Pb<sup>2+</sup>

引用本文: 杨瑞, 康天放, 鲁理平, 叶青. 基于DNA酶/AuNPs-GO传感器方波伏安法高灵敏检测Pb²⁺[J]. 分析化学, 2021, 49(2): 309-317. doi: 10.19756/j.issn.0253-3820.201231 shu

Citation: YANG Rui, KANG Tian-Fang, LU Li-Ping, YE Qing. Highly Sensitive Detection of Pb²⁺ Based on DNAzyme/AuNPs-GO Sensor by Square-Wave Voltammetry[J]. Chinese Journal of Analytical Chemistry, 2021, 49(2): 309-317. doi: 10.19756/j.issn.0253-3820.201231 shu

基于DNA酶/AuNPs-GO传感器方波伏安法高灵敏检测Pb²⁺

北京工业大学区域大气复合污染防治北京市重点实验室, 环境与能源工程学院, 北京 100124

通讯作者: 康天放,E-mail:kangtf@bjut.edu.cn

基金项目:
北京市科技计划项目（No.Z171100002217002）和教育部博士点基金（No.20131103110011）资助。

摘要: 制备了金纳米粒子-氧化石墨烯（AuNPs-GO）复合物，采用透射电镜（TEM）和紫外-可见吸收光谱（UV-Vis）对其进行了表征。将此复合物修饰到玻碳电极（GCE）表面，再通过Au—S键将巯基修饰的富含鸟嘌呤的DNA链（T30695）自组装到电极表面的金纳米粒子上。T30695可与Pb²⁺结合形成稳定的平行G-四链体，此G-四链体再与氯化血红素（Hemin）结合形成具有过氧化物酶活性的DNA酶（DNAzyme），基于此制得Pb²⁺敏感的DNA酶电化学传感器。形成的DNA酶可催化H₂O₂氧化对苯二酚（HQ），实现电化学信号的放大和Pb²⁺的高灵敏检测。在优化的实验条件下，在5.0×10^-9~1.0×10^-6 mol/L浓度范围内，传感器的方波伏安（SWV）峰电流与Pb²⁺浓度的对数值有良好的线性关系，检出限为1.0×10^-9 mol/L（S/N=3）。将构建的传感器用于实际水样中Pb²⁺的检测，加标回收率在96%-105%之间。本传感器具有良好的重现性、稳定性和选择性。

关键词:

方波伏安法
/ 传感器
/ DNA酶
/ 铅离子

English

Highly Sensitive Detection of Pb²⁺ Based on DNAzyme/AuNPs-GO Sensor by Square-Wave Voltammetry

Key Laboratory of Beijing on Regional Air Pollution Control, College of Environmental and Energy Engineering, Beijing University of Technology, Beijing 100124, China

Corresponding author: KANG Tian-Fang, kangtf@bjut.edu.cn

Received Date: 25 April 2020
Revised Date: 18 November 2020
Fund Project:
Supported by the Scientific Technological Project Foundation of Beijing Municipal Scientific Technological Commission (No.Z171100002217002) and the Doctoral Fund of China Education Ministry (No.20131103110011).

Abstract: The composite of gold nanoparticles-graphene oxide(AuNPs-GO) was prepared and characterized by transmission electron microscope (TEM) and ultraviolet-visible(UV-vis) spectrophotometry, respectively. The composite was modified on the surface of a glassy carbon electrode (GCE). The mercapto-modified and guanine-rich DNA strand (T30695) was self-assembled on to gold nanoparticles on the surface of the electrode via Au-S bond. T30695 could combine with lead ions (Pb²⁺) to form a stable parallel G-quadruplex. The G-quadruplex further combined with hemin to form DNAzyme with peroxidase activity. On the basis of this, a DNAzyme electrochemical sensor for Pb²⁺ detection was prepared. The DNAzyme could catalyze the oxidation reaction of hydrogen peroxidetowardshydroquinone (HQ) to achieve the amplification of electrochemical signal and highly sensitive detection of Pb²⁺. Under the optimum conditions, the peak current of square-wave voltammetry (SWV) was linearly dependent on the logarithm of Pb²⁺ concentration ranging from 5.0×10^-9 mol/L to 1.0×10^-6 mol/L with a detection limit of 1.0×10^-9 mol/L(S/N=3). The recovery of Pb²⁺ in real water sample was 96%-105%. The sensor possessed good reproducibility, stability and selectivity to Pb²⁺.

Key words:

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

基于DNA酶/AuNPs-GO传感器方波伏安法高灵敏检测Pb²⁺

通讯作者: 康天放,E-mail:kangtf@bjut.edu.cn

English

Highly Sensitive Detection of Pb²⁺ Based on DNAzyme/AuNPs-GO Sensor by Square-Wave Voltammetry

Corresponding author: KANG Tian-Fang, kangtf@bjut.edu.cn

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

基于DNA酶/AuNPs-GO传感器方波伏安法高灵敏检测Pb2+

通讯作者: 康天放,E-mail:kangtf@bjut.edu.cn

English

Highly Sensitive Detection of Pb2+ Based on DNAzyme/AuNPs-GO Sensor by Square-Wave Voltammetry

Corresponding author: KANG Tian-Fang, kangtf@bjut.edu.cn

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

基于DNA酶/AuNPs-GO传感器方波伏安法高灵敏检测Pb²⁺

Highly Sensitive Detection of Pb²⁺ Based on DNAzyme/AuNPs-GO Sensor by Square-Wave Voltammetry

CCS Chemistry：颜徐州、鲍哲南：你的皮肤可能是一片SPMs