毛细管电泳与核酸适配体高效筛选

朱超 赵新颖 杨歌 屈锋

引用本文: 朱超,  赵新颖,  杨歌,  屈锋. 毛细管电泳与核酸适配体高效筛选[J]. 分析化学, 2020, 48(5): 583-589. doi: 10.19756/j.issn.0253-3820.201173 shu
Citation:  ZHU Chao,  ZHAO Xin-Ying,  YANG Ge,  QU Feng. Capillary Electrophoresis Involving in High Efficiency Screening for Aptamers[J]. Chinese Journal of Analytical Chemistry, 2020, 48(5): 583-589. doi: 10.19756/j.issn.0253-3820.201173 shu

毛细管电泳与核酸适配体高效筛选

  • 基金项目:

    本文系国家自然科学基金项目(Nos.20875009,21175011,21375008,21675012,21874010,21827810)和北京市科学技术协会2020-2022年度青年人才托举工程项目资助

摘要: 指数富集配体系统进化(SELEX)技术是核酸适配体筛选的通用技术,精准高效的筛选方法和筛选策略设计是成功筛选适配体的关键。本文概述了本课题组自2007年以来,应用毛细管电泳(CE)技术研究核酸适配体高效筛选的有关工作,包括CE在核酸适配体筛选前、筛选中及筛选后各环节中的分析分离应用、不同蛋白靶的分类及筛选策略、多种CE筛选模式和多尺度靶标的核酸适配体筛选。同时,探讨了SELEX中存在的问题,并对其发展前景进行了展望。

English


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  • 收稿日期:  2020-03-31
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