毛细管电泳与核酸适配体高效筛选

引用本文: 朱超, 赵新颖, 杨歌, 屈锋. 毛细管电泳与核酸适配体高效筛选[J]. 分析化学, 2020, 48(5): 583-589. doi: 10.19756/j.issn.0253-3820.201173 shu

Citation: ZHU Chao, ZHAO Xin-Ying, YANG Ge, QU Feng. Capillary Electrophoresis Involving in High Efficiency Screening for Aptamers[J]. Chinese Journal of Analytical Chemistry, 2020, 48(5): 583-589. doi: 10.19756/j.issn.0253-3820.201173 shu

毛细管电泳与核酸适配体高效筛选

朱超 ^1, ,
赵新颖 ^2, ,
杨歌 ^1, ,
屈锋 ^1,

1.
北京理工大学生命学院, "分子医学与生物诊疗"工业和信息化部重点实验室, 北京 100081;
2.
北京市理化分析测试中心, 北京 100089
基金项目:
本文系国家自然科学基金项目（Nos.20875009，21175011，21375008，21675012，21874010，21827810）和北京市科学技术协会2020-2022年度青年人才托举工程项目资助

摘要: 指数富集配体系统进化（SELEX）技术是核酸适配体筛选的通用技术，精准高效的筛选方法和筛选策略设计是成功筛选适配体的关键。本文概述了本课题组自2007年以来，应用毛细管电泳（CE）技术研究核酸适配体高效筛选的有关工作，包括CE在核酸适配体筛选前、筛选中及筛选后各环节中的分析分离应用、不同蛋白靶的分类及筛选策略、多种CE筛选模式和多尺度靶标的核酸适配体筛选。同时，探讨了SELEX中存在的问题，并对其发展前景进行了展望。

关键词:

English

Capillary Electrophoresis Involving in High Efficiency Screening for Aptamers

1.
School of Life Science, Beijing Institute of Technology, Key Laboratory of Molecular Medicine and Biological Diagnosis, Ministry of Industry and Information Technology, Beijing 100081, China;
2.
Beijing Center for Physical and Chemical Analysis, Beijing 100089, China
Received Date: 31 March 2020
Revised Date: 16 April 2020
Fund Project:
This work was supported by the National Natural Science Foundation of China (Nos. 20875009, 21175011, 21375008, 21675012, 21874010, 21827810) and the Young Elite Scientist Sponsorship Program of Beijing Association for Science and Technology (2020-2022).

Abstract: Systematic evolution of ligands by exponential enrichment (SELEX) is a universal technology for aptamers’ screening. Accurate and efficient screening methods and strategy design are the key to successful screening of aptamers. This article briefly summarizes our works of capillary electrophoresis involving in the efficient screening of aptamers since 2007. The review also introduces the employment of capillary electrophoresis in pre-screening, screening and post-screening SELEX process, classification and screening strategies against different protein targets, multiple capillary electrophoresis screening modes, and aptamers screening for multi-scale targets. Finally, combined with the current research progress of aptamer screening in the world, some remaining issues are proposed.

Key words:

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毛细管电泳与核酸适配体高效筛选

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Capillary Electrophoresis Involving in High Efficiency Screening for Aptamers

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