谷胱甘肽的宽频太赫兹光谱研究

引用本文: 郭聪, 朱中杰, 沈建雄, 张宗昌, 张建兵, 赵德明, 赵红卫. 谷胱甘肽的宽频太赫兹光谱研究[J]. 分析化学, 2020, 48(10): 1367-1374. doi: 10.19756/j.issn.0253-3820.201148 shu

Citation: GUO Cong, ZHU Zhong-Jie, SHEN Jian-Xiong, ZHANG Zong-Chang, ZHANG Jian-Bing, ZHAO De-Ming, ZHAO Hong-Wei. Broadband Terahertz Spectroscopy of Glutathione[J]. Chinese Journal of Analytical Chemistry, 2020, 48(10): 1367-1374. doi: 10.19756/j.issn.0253-3820.201148 shu

谷胱甘肽的宽频太赫兹光谱研究

1.
浙江工业大学化工学院, 杭州 310014;
2.
中国科学院上海高等研究院张江实验室, 上海 201210;
3.
中国科学院上海应用物理研究所, 上海 201800
基金项目:
本文系中国科学院微观界面物理与探测重点实验室项目和国防科技创新特区项目资助

摘要: 谷胱甘肽（Glutathione）的构型构象对其发挥生物学功能具有重要意义。本研究利用空气等离子体太赫兹时域光谱（THz-TDS）获得了还原型谷胱甘肽（GSH）和氧化型谷胱甘肽（GSSG）在0.5~12.0 THz波段的吸收光谱，结果表明，GSH在太赫兹波段有丰富的特征吸收峰，而GSSG呈现单调无特征的吸收曲线。粉末X射线衍射（PXRD）结果表明，GSH具有一定的晶型结构而GSSG为无定形态，提示太赫兹光谱对物质晶体结构有敏感响应。利用密度泛函理论（DFT）对GSH晶胞结构进行计算和太赫兹振动光谱分析，结果表明，GSH分子能形成丰富的氢键，这些氢键网络有助于约束柔性肽分子并使分子有序地堆叠形成晶体。晶格和氢键与太赫兹波作用产生共振吸收，GSH的太赫兹光谱中不同吸收峰对应分子不同集体振动或局域振动，并且与氢键的振动密切相关。本研究结果有助于加深GSH分子构型构象和分子弱相互作用的认识。

关键词:

English

Broadband Terahertz Spectroscopy of Glutathione

1.
College of Chemical Engineering, Zhejiang University of Technology, Hangzhou 310014, China;
2.
Zhangjiang Laboratory, Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai 201210, China;
3.
Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China
Received Date: 21 March 2020
Revised Date: 03 July 2020
Fund Project:
This work was supported by the Main Direction Program of Knowledge Innovation and Open Project Program of Key Laboratory of Interfacial Physics and Technology of Chinese Academy of Sciences and the National Defense Science and Technology Innovation Special Zone.

Abstract: The conformation of glutathione is of great significance for its biological function. In this study, the absorption spectra of reduced (GSH) and oxidized (GSSG) glutathione in the frequency range of 0.5-12 THz were obtained by the air plasma terahertz time-domain spectroscopy (THz-TDS). The results demonstrated that GSH had rich characteristic absorption peaks in the THz region, while GSSG presented a monotonous absorption spectrum without obvious peaks. Combined with powder X-ray diffraction (PXRD), GSH had a certain crystal structure and GSSG was amorphous, suggesting that the THz spectroscopy was sensitive to the molecular structure and crystal form. The density functional theory (DFT) calculation based on the GSH crystal structure was performed to simulate the THz absorption spectrum and analyze the vibrational property. The result showed that GSH molecule could form rich intra-and inter-molecular hydrogen bonds, which helped to constrain flexible peptide. Lattice vibration and hydrogen bonding of GSH can effectively resonate with THz waves. The different THz absorption peaks of GSH correspond to different collective or local molecular vibrations which were closely related to the hydrogen bonding interactions. The study may help to deepen the understanding of GSH molecular conformation and weak interactions.

Key words:

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1.
沈阳化工大学材料科学与工程学院沈阳 110142

谷胱甘肽的宽频太赫兹光谱研究

English

Broadband Terahertz Spectroscopy of Glutathione

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

谷胱甘肽的宽频太赫兹光谱研究

English

Broadband Terahertz Spectroscopy of Glutathione

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

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CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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