基于高效液相色谱-气相色谱联用技术的矿物油分析研究进展

引用本文: 武彦文, 李冰宁, 刘玲玲, 欧阳杰. 基于高效液相色谱-气相色谱联用技术的矿物油分析研究进展[J]. 分析化学, 2021, 49(3): 341-349. doi: 10.19756/j.issn.0253-3820.201134 shu

Citation: WU Yan-Wen, LI Bing-Ning, LIU Ling-Ling, OUYANG Jie. Research Progress of Analysis of Mineral Oil Hydrocarbons Using On-Line High Performance Liquid Chromatography Coupled with Gas Chromatography[J]. Chinese Journal of Analytical Chemistry, 2021, 49(3): 341-349. doi: 10.19756/j.issn.0253-3820.201134 shu

基于高效液相色谱-气相色谱联用技术的矿物油分析研究进展

武彦文 ^{1,
,} ,
李冰宁 ^1, ,
刘玲玲 ^1, ,
欧阳杰 ^2,

1.
北京市理化分析测试中心, 北京市食品安全测试工程技术研究中心, 北京 100094;
2.
北京林业大学生物科学与技术学院食品科学与工程系, 北京 100083

通讯作者: 武彦文,E-mail:wu_yanwen@163.com

基金项目:
北京市自然科学基金项目（No.2182020）和北京市科学技术研究院改革与发展经费培育类项目（No.PY2020JC38）资助。

摘要: 近年来，利用高效液相色谱-气相色谱联用技术（HPLC-GC）分析矿物油的研究发展迅速。矿物油源于石油与合成油，是涵盖一定碳数范围的碳氢化合物，主要分为烷烃和芳烃两大类。矿物油可通过多种途径迁移至食品和环境中，影响人类健康，然而其分析检测面临较大挑战。HPLC-GC （配备氢火焰离子化检测器）通过将净化、分离与检测相结合，很好地解决了矿物油分析中的选择性和灵敏度等问题。该技术的核心是通过保留间隙、Y形件接口和溶剂蒸气排出实现了GC大体积进样，将常规HPLC的洗脱流分全部转移至GC分析，将矿物油的分析灵敏度提高了两个数量级以上；同时避免了分析过程的污染引入，保证了分析的重现性和准确度。本文详细阐述了HPLC-GC的发展历程以及HPLC与GC分析条件的相互匹配，评述了基于HPLC-GC联用技术的干扰物去除与矿物油富集方法，并对HPLC-GC在矿物油分析中应用前景和发展趋势进行了展望。

关键词:

English

Research Progress of Analysis of Mineral Oil Hydrocarbons Using On-Line High Performance Liquid Chromatography Coupled with Gas Chromatography

1.
Beijing Center for Physical & Chemical Analysis, Beijing Food Safety Analysis and Testing Engineering Research Center, Beijing 100094, China;
2.
Department of Food Science and Engineering, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083 China

Corresponding author: WU Yan-Wen, wu_yanwen@163.com

Received Date: 16 March 2020
Revised Date: 22 September 2020
Fund Project:
Beijing Natural Science Foundation (No.2182020) and the Beijing Academy of Science and Technology-Reform and Development Project (No.PY2020JC38).

Abstract: In the past decades, the application of on-line high performance liquid chromatography coupled with gas chromatography (HPLC-GC) has rapidly developed in quantitative analysis of mineral oil hydrocarbons (MOH). MOH are derived from petroleum and synthetic fuels and cover a large range of boiling points, mainly consisting of mineral oil saturated hydrocarbons (MOSH) and aromatic hydrocarbons (MOAH). Usually, MOH are migrated from variety of sources to various foods, thus existing in foods widely, which have been an adverse impact on human health. However, the analysis of MOH is challenging due to the limited selectivity and sensitivity of hydrogen flame ionization detector (FID). The on-line HPLC-GC combines high separation efficiency of HPLC and equal-carbon-response detection of GC-FID, which solves the problem of selectivity and sensitivity of MOH analysis. The innovation of retention gap, Y-piece interface and solvent vapor exit equipped in HPLC-GC employ large volume injection into GC-FID. Since the elution fractions from HPLC are completely transferred to GC, the analytical sensitivity of MOH is enhanced by more than two orders of magnitude and the contaminants introduced are minimized during the analytical procedure, ensuring reproducibility and accuracy of the MOH analysis. In this paper, the research history of interfaces used in on-line HPLC-GC and the matching conditions of HPLC and GC were described in detail and the sample preparation methods of interference removal and MOH enrichment based on HPLC-GC were reviewed. The limitations of HPLC-GC were summarized and the future application of the technology and the development trend in MOH analysis were prospected in the end.

Key words:

On-line high performance liquid chromatography coupled with gas chromatography
/ Mineral oil hydrocarbons
/ Foods
/ Analysis
/ Interference substances
/ Review

1. [1]
  European Food Safety Authority (EFSA). EFSA J., 2012, 10(6):2704.
2. [2]
  BIEDERMANN M, GROB K. J. Chromatogr. A, 2012, 1255:56-75.
3. [3]
  MORET S, BARP L, GROB K, CONTE L S. Food Chem., 2011, 129:1898-1903.
4. [4]
  MORET S, BARP L, PURCARO G, CONTE L S. J. Chromatogr. A, 2012, 1243:1-5.
5. [5]
  FISELIER K, GRUNDBÖCK F, SCHÖN K, KAPPENSTEIN O, PFAFF K, HUTZLER C, LUCH A, GROB K. J. Chromatogr. A, 2013, 1271:192-200.
6. [6]
  MAJORS R E. J. Chromatogr. Sci., 1980, 18(10):571-579.
7. [7]
  GROB K. On-line Coupled LC-GC. Hüthig, Heidelberg, 1991:10.
8. [8]
  GROB K, FROHLICH D, SCHILLING B, NEUKOM H P, NAGELI P. J. Chromatogr. A, 1984, 295:55-61.
9. [9]
  BIEDERMANN M, GROB K, MEIER W. J. High Resolut. Chromatogr., 1989, 12:591-598.
10. [10]
  BOSELLI E, GROLIMUND B, GROB K. J. High Resolut. Chromatogr., 1998, 21:355-362.
11. [11]
  BIEDERMANN M, GROB K. J. Chromatogr. A, 2013, 1281:106-114.
12. [12]
  GROB K, BIEDERMANN M. J. Chromatogr. A, 1996, 750:11-23.
13. [13]
  BOSELLI E, GROB K, LERCKER G. J. High Resolut. Chromatogr., 1999, 22:149-152.
14. [14]
  GROB K, SCHMARR H G, MOSANDL A. J. High Resolut. Chromatogr., 1989, 12:375-382.
15. [15]
  GROB K. J. Chromatogr. A, 2000, 892:407-420.
16. [16]
  DUGO P, DUGO G, MONDELLO L. LC-GC Eur., 2003, 16(12A):35-43.
17. [17]
  BIEDERMANN M, GROB K. J. Chromatogr. A, 2009, 1216:8652-8658.
18. [18]
  PURCARO G, ZOCCALI M, TRANCHIDA P Q, BARP L, MORET S, CONTE L, DUGO P, MONDELLO L. Anal. Bioanal. Chem., 2013, 405:1077-1084.
19. [19]
  DIN EN 16995-2017, Foodstuffs-Vegetable Oils and Foodstuff on Basis of Vegetable Oils-Determination of Mineral Oil Saturated Hydrocarbons (MOSH) and Mineral Oil Aromatic Hydrocarbons (MOAH) with On-line HPLC-GC-FID Analysis. British Standard.
20. [20]
  BRATINOVA S, HOEKSTRA E (Editors). Luxembourg:Publications Office of the European Union, 2019, ISBN 978-92-76-00172-0.
21. [21]
  DIN SPEC 5010:2018-05, Testing of Paper and Board-Determination of the Transfer of Mineral oil Hydrocarbons from Food Contact Material Manufactured with Portions of Recycled Pulp.
22. [22]
  ISO 17780:2015, Animal and Vegetable Fats and Oils-Determination of Aliphatic Hydrocarbons in Vegetable Oils.
23. [23]
  BENNETT B, LARTER S R. Anal. Chem. 2000, 72:1039-1044.
24. [24]
  BIEDERMANN M, MUNOZ C, GROB K. J. Chromatogr. A, 2017, 1521:140-149.
25. [25]
  BIEDERMANN M, GROB K. J. Chromatogr. A, 2012, 1255:76-99.
26. [26]
  LIU Ling-Ling, WU Yan-Wen, LI Bing-Ning, WANG Yu, YANG Yi-Fan, ZU Wen-Chuan, WANG Xin-Xin. Chin. J. Anal. Chem., 2016, 44(9):1419-1424. 刘玲玲, 武彦文, 李冰宁, 汪雨, 杨一帆, 祖文川, 王欣欣. 分析化学, 2016, 44(9):1419-1424.
27. [27]
  FISELIER K, FIORINI D, GROB K. Anal. Chim. Acta, 2009, 634:96-101.
28. [28]
  FISELIER K, FIORINI D, GROB K. Anal. Chim. Acta, 2009, 634:102-109.
29. [29]
  FISELIER K, GROB K. Eur. Food Res. Technol., 2009, 229:679-688.
30. [30]
  ZURFLUH M, BIEDERMANN M, GROB K. J. Verbr. Lebensm., 2014, 9:61-69.
31. [31]
  BROER W. Automated Solutions for the Determination of Contaminants in Food-from Sample Prep to Analysis. 9^th International Symposium on Recent Advances in Food Analysis, Pague, Czech Republic, 2019, Nov 5^th-8^th, 59.
32. [32]
  MORET S, GROB K, CONTE L S. J. Chromatogr. A, 1996, 750:361-368.
33. [33]
  ZOCCALI M, BARP L, BECCARIA M, SCIARRONE D, PURCARO G, MONDELLO L. J. Sep. Sci., 2016, 39:623-631.
34. [34]
  BIEDERMANN M, FISELIER K, GROB K. J. Agric. Food Chem., 2009, 57:8711-8721.
35. [35]
  NESTOLA M, SCHMIDT T C. J. Chromatogr. A, 2017, 1505:69-76.
36. [36]
  VAN HEYST A, VANLANCKER M, VERCAMMEN J, HOUWE K, MERTENS B, ELSKENS M, VAN HOECK E. Food Add. Contam. A, 2018, 35(6):2062-2075.
37. [37]
  KOSTER S, VARELA J, STADLER R H, MOULIN J, CRUZ-HERNANDEZ C, HIELSCHER J, LESUEUR C, ROÏZ J, SIMIAN H. Food Add. Contam. A, 2020, 37(1):69-83.
38. [38]
  BIEDERMANN M, MUNOZ C, GROB K. J. Chromatogr. A, 2020, 1624:461236.
39. [39]
  BIEDERMANN-BREM S, KASPRICK N, SIMAT T J, GROB K. Food Add. Contam. A, 2012, 29(3):449-460.
40. [40]
  ZHANG S C, LIU L L, LI B N, XIE Y Q, OUYANG J, WU Y W. Food Add. Contam. A, 2019, 36(8):1261-1272.
41. [41]
  LOMMATZSCH M, BIEDERMANN M, SIMAT T J, GROB K. J. Chromatogr. A, 2015, 1402:94-101.
42. [42]
  LOMMATZSCH M, BIEDERMANN M, GROB K, SIMAT T J. Food Add. Contam. A, 2016, 33(3):473-488.
43. [43]
  European Food Safety Authority (EFSA), Arcella D, Baert K, Binaglia M. Rapid Risk Assessment on the Possible Risk for Public Health Due to the Contamination of Infant Formula and Follow-on Formula by Mineral Oil Aromatic Hydrocarbons (MOAH). EFSA Supporting Publicatons, 2019, EN-1741.
44. [44]
  BIEDERMANN M, GROB K. J. Sep. Sci., 2009, 32:3726-3737.
45. [45]
  BIEDERMANN M, GROB K. J. Chromatogr. A, 2015, 1375:146-153.
46. [46]
  ZOCCALI M, TRANCHIDA P Q, MONDELLO L. Anal. Chim. Acta, 2019, 1048:221-226.
47. [47]
  KOCH M, BECKER E, PÄCH M, KVHN S, KIRCHHOFF E. J. Sep. Sci. 2020, 43(6):1089-1099.
48. [48]
  PURCARO G, TRANCHIDA P Q, BARP L, MORET S, CONTE L S, MONDELLO L. Anal. Chim. Acta, 2013, 773:97-104.

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基于高效液相色谱-气相色谱联用技术的矿物油分析研究进展

通讯作者: 武彦文,E-mail:wu_yanwen@163.com

English

Research Progress of Analysis of Mineral Oil Hydrocarbons Using On-Line High Performance Liquid Chromatography Coupled with Gas Chromatography

Corresponding author: WU Yan-Wen, wu_yanwen@163.com

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

基于高效液相色谱-气相色谱联用技术的矿物油分析研究进展

通讯作者: 武彦文,E-mail:wu_yanwen@163.com

English

Research Progress of Analysis of Mineral Oil Hydrocarbons Using On-Line High Performance Liquid Chromatography Coupled with Gas Chromatography

Corresponding author: WU Yan-Wen, wu_yanwen@163.com

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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