Citation: WANG Ju-Duo, SONG Jia-Feng, LI Chang, DAI Xin-Hua, FANG Xiang, GONG Xiao-Yun, YE Zi-Hong. Recent Advances in Single Cell Analysis Methods Based on Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, ;2020, 48(8): 969-980. doi: 10.19756/j.issn.0253-3820.201128 shu

Recent Advances in Single Cell Analysis Methods Based on Mass Spectrometry

1.
College of Life Sciences, China Jiliang University, Hangzhou 310018, China;
2.
Center for Advanced Measurement Science, National Institute of Metrology, Beijing 100029, China

Received Date: 12 March 2020
Revised Date: 9 June 2020

Fund Project: This work was supported by the National Natural Science Foundation of China (Nos.21605135, 21927812).

The heterogeneity among individual cells is of great importance for organisms to achieve different biological functions. Investigations deep into the mechanism of some biological phenomena rely on the analysis of the chemical compositions of single cells. Unfortunately, the successful analysis of single cells is technically difficult to achieve due to the complex composition of single cells, the very limited amount of materials in single cells and the huge difference among different metabolites in their concentrations. Mass spectrometry (MS) has been widely used in single cell analysis in recent years due to its high sensitivity, high specificity and capability for structure identification. Methods based on MS have been developed for the analysis of different metabolites in single cells. From the perspective of ionization techniques, there are mainly three single cell analysis methods, including nano-electrospray ionization (Nano-ESI) based method, laser desorption ionization (LDI) based method and secondary ion MS (SIMS) based method. This review summarizes the up-to-date developments of these methods and their future trends.
- Mass spectrometry,
- Single cell analysis,
- Ionization,
- Nano-electrospray ionization,
- Laser desorption ionization,
- Secondary ion mass spectrometry,
- Review
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1.
沈阳化工大学材料科学与工程学院沈阳 110142