小分子靶标的核酸适配体筛选研究进展

引用本文: 王子健, 陈尔凝, 杨歌, 赵新颖, 屈锋. 小分子靶标的核酸适配体筛选研究进展[J]. 分析化学, 2020, 48(5): 573-582. doi: 10.19756/j.issn.0253-3820.201101 shu

Citation: WANG Zi-Jian, CHEN Er-Ning, YANG Ge, ZHAO Xin-Ying, QU Feng. Research Advances of Aptamers Selection for Small Molecule Targets[J]. Chinese Journal of Analytical Chemistry, 2020, 48(5): 573-582. doi: 10.19756/j.issn.0253-3820.201101 shu

小分子靶标的核酸适配体筛选研究进展

王子健 ^1, ,
陈尔凝 ^2, ,
杨歌 ^1, ,
赵新颖 ^2, ,
屈锋 ^1,

1.
北京理工大学生命学院, "分子医学与生物诊疗"工业和信息化部重点实验室, 北京 100081;
2.
北京市理化分析测试中心, 北京 100089
基金项目:
本文系国家自然科学基金项目（Nos.21675012，21274010，21874810）资助

摘要: 核酸适配体（Aptamer）作为新型识别分子，在小分子传感分析和检测中有很大的应用潜力。小分子的Aptamer筛选是其应用的前提。本文综述了2015~2019年，有关毒素、抗生素、激素等近80种小分子的Aptamer的筛选研究进展，归纳总结了近百条Aptamer序列和平衡解离常数K_D及其测定方法，简要介绍了基于氧化石墨烯、人类基因组、石英晶体微天平以及计算机辅助筛选等改进型指数富集配体系统进化技术。

关键词:

English

Research Advances of Aptamers Selection for Small Molecule Targets

1.
School of Life Science, Beijing Institute of Technology, Key Laboratory of Molecular Medicine and Biological Diagnosis, Ministry of Industry and Information Technology, Beijing 100081, China;
2.
Beijing Center for Physical and Chemical Analysis, Beijing 100089, China
Received Date: 28 February 2020
Revised Date: 20 April 2020
Fund Project:
This work was supported by the National Natural Science Foundation of China (Nos. 21675012, 21874010, 21827810).

Abstract: Aptamer, as a novel recognition molecule, shows great potential in small molecule sensing analysis and detection. Selection of aptamers for small molecules is a pre-requisite for their application. This paper summarizes nearly 100 aptamers which are sequenced against 80 kinds of small molecular targets (e.g. toxins, antibiotics and hormones) from 2015 to 2019. Their equilibrium dissociation constants (K_D) and determination methods are also summarized. In addition, the development and enhancements of systematic evolution of ligands by exponential enrichment (SELEX) methods based on graphene oxide, human genome, quartz crystal microbalance and in silico are briefly introduced.

Key words:

Aptamer
/ Small molecule
/ Systematic evolution of ligands by exponential enrichment
/ Review

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1.
沈阳化工大学材料科学与工程学院沈阳 110142

小分子靶标的核酸适配体筛选研究进展

English

Research Advances of Aptamers Selection for Small Molecule Targets

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