基质辅助激光解析质谱成像可视化分析三七皂苷空间分布

引用本文: 刘芳, 赵瀚森, 孙公伟, 宋哲, 徐富建, 马明英, 张四纯. 基质辅助激光解析质谱成像可视化分析三七皂苷空间分布[J]. 分析化学, 2020, 48(7): 881-888. doi: 10.19756/j.issn.0253-3820.201066 shu

Citation: LIU Fang, ZHAO Han-Sen, SUN Gong-Wei, SONG Zhe, XU Fu-Jian, MA Ming-Ying, ZHANG Si-Chun. Visualization Analysis of Spatial Distribution of Notoginsenoside by Matrix-assisted Laser Desorption Ionization Mass Spectrometry Imaging[J]. Chinese Journal of Analytical Chemistry, 2020, 48(7): 881-888. doi: 10.19756/j.issn.0253-3820.201066 shu

基质辅助激光解析质谱成像可视化分析三七皂苷空间分布

刘芳 ^1,2, ,
赵瀚森 ^2, ,
孙公伟 ^2, ,
宋哲 ^2, ,
徐富建 ^2, ,
马明英 ^2, ,
张四纯 ^2,

1.
文山学院化学与工程学院, 文山 663099;
2.
清华大学化学系, 北京 100084
基金项目:
本文系国家自然科学基金项目（No.21621003）资助

摘要: 根据2，5-二羟基苯甲酸（DHB）、α-氰基-4-羟基肉桂酸（CHCA）、石墨烯（GR）、氧化石墨烯（GO）和碳纳米管（CNT）5种基质混合人参皂苷Rg₁、人参皂苷Rb₁以及三七皂苷R1 3种标准品得到的质谱信号，选取DHB为基质。将20 μm厚度的三七根茎冷冻切片真空干燥40 min后，在循环60次、喷雾强度30%、喷雾时间1 s、孵育时间40 s、干燥时间60 s的条件下，将DHB均匀覆盖于切面。质谱数据采集质量范围为m/z 0-1500 Da，使用PEG-600进行质荷比校准，以1000 Hz频率的正离子反射模式进行基质辅助激光解吸质谱成像（MALDI-MSI），观察到10种皂苷在三七根茎木栓层、韧皮部、木质部以及髓部的3种空间分布情况，同时对质谱数据进行t-分布领域嵌入算法分析（t-SNE），实现了2年生和3年生三七根茎的区分。本研究为三七根茎组织中代谢物的表征提供了原位、可视化的方法，也为三七根茎的特异性提取提供了有价值的信息。

关键词:

English

Visualization Analysis of Spatial Distribution of Notoginsenoside by Matrix-assisted Laser Desorption Ionization Mass Spectrometry Imaging

1.
College of Chemistry and Engineering, Wenshan University, Wenshan 663099, China;
2.
Department of Chemistry, Tsinghua University, Beijing 100084, China
Received Date: 10 February 2020
Revised Date: 20 April 2020
Fund Project:
This work was supported by the National Natural Science Foundation of China (No.21621003).

Abstract: Mass spectrometry signals were obtained from the mixtures of five matrixes and three standard product combinations. The five matrixes were 2,5-dihydroxybenzoic acid (DHB), α-cyano-4-hydroxycinnamic acid (CHCA), graphene (GR), graphene oxide (GO) and carbon nanotubes (CNT), while the three standard products were ginsenoside Rg₁, ginsenoside Rb₁ and notoginsenoside R1. DHB was utilized as a suitable matrix. After vacuum drying the 20 μm thick frozen sections of Panax notoginseng rhizome for 40 min, DHB was evenly coated on the section surface over 60 cycles, with a spray strength of 30%, a spray time of 1 s, an incubation time of 40 s and a drying time of 60 s. The data acquisition quality range was m/z 0-1500. PEG-600 was used for mass/charge ratio calibration and matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI-MSI) was performed in 1000 Hz positive ion reflection mode. The spatial distribution of ten saponins in the cork layer, phloem, xylem and pith of Panax notoginseng rhizome was obtained. The t-distributed stochastic neighbor embedding (t-SNE) algorithm was used to analyze the data. A distinction between 2-year and 3-year panax notoginseng rhizomes was realized. This study provided an in-situ and visual method for the characterization of metabolites in the rhizome of Panax notoginseng and also provided valuable information for obtaining information on the specificity of Panax notoginseng.

Key words:

Matrix-assisted laser desorption/ionization
/ Mass spectrometry imaging
/ Panax notoginseng rhizome
/ Saponin

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沈阳化工大学材料科学与工程学院沈阳 110142

基质辅助激光解析质谱成像可视化分析三七皂苷空间分布

English

Visualization Analysis of Spatial Distribution of Notoginsenoside by Matrix-assisted Laser Desorption Ionization Mass Spectrometry Imaging

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中国化学会秘书处

基质辅助激光解析质谱成像可视化分析三七皂苷空间分布

English

Visualization Analysis of Spatial Distribution of Notoginsenoside by Matrix-assisted Laser Desorption Ionization Mass Spectrometry Imaging

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CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

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