Citation: LIU Fang, ZHAO Han-Sen, SUN Gong-Wei, SONG Zhe, XU Fu-Jian, MA Ming-Ying, ZHANG Si-Chun. Visualization Analysis of Spatial Distribution of Notoginsenoside by Matrix-assisted Laser Desorption Ionization Mass Spectrometry Imaging[J]. Chinese Journal of Analytical Chemistry, ;2020, 48(7): 881-888. doi: 10.19756/j.issn.0253-3820.201066 shu

Visualization Analysis of Spatial Distribution of Notoginsenoside by Matrix-assisted Laser Desorption Ionization Mass Spectrometry Imaging

1.
College of Chemistry and Engineering, Wenshan University, Wenshan 663099, China;
2.
Department of Chemistry, Tsinghua University, Beijing 100084, China

Received Date: 10 February 2020
Revised Date: 20 April 2020

Fund Project: This work was supported by the National Natural Science Foundation of China (No.21621003).

Mass spectrometry signals were obtained from the mixtures of five matrixes and three standard product combinations. The five matrixes were 2,5-dihydroxybenzoic acid (DHB), α-cyano-4-hydroxycinnamic acid (CHCA), graphene (GR), graphene oxide (GO) and carbon nanotubes (CNT), while the three standard products were ginsenoside Rg₁, ginsenoside Rb₁ and notoginsenoside R1. DHB was utilized as a suitable matrix. After vacuum drying the 20 μm thick frozen sections of Panax notoginseng rhizome for 40 min, DHB was evenly coated on the section surface over 60 cycles, with a spray strength of 30%, a spray time of 1 s, an incubation time of 40 s and a drying time of 60 s. The data acquisition quality range was m/z 0-1500. PEG-600 was used for mass/charge ratio calibration and matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI-MSI) was performed in 1000 Hz positive ion reflection mode. The spatial distribution of ten saponins in the cork layer, phloem, xylem and pith of Panax notoginseng rhizome was obtained. The t-distributed stochastic neighbor embedding (t-SNE) algorithm was used to analyze the data. A distinction between 2-year and 3-year panax notoginseng rhizomes was realized. This study provided an in-situ and visual method for the characterization of metabolites in the rhizome of Panax notoginseng and also provided valuable information for obtaining information on the specificity of Panax notoginseng.
- Matrix-assisted laser desorption/ionization,
- Mass spectrometry imaging,
- Panax notoginseng rhizome,
- Saponin
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沈阳化工大学材料科学与工程学院沈阳 110142