基于对半胱胺保护的金纳米粒子催化活性的抑制检测氰根离子

引用本文: 赵丹, 李娜, 许艳杰, 黄晶晶, 陈传霞. 基于对半胱胺保护的金纳米粒子催化活性的抑制检测氰根离子[J]. 分析化学, 2020, 48(7): 889-895. doi: 10.19756/j.issn.0253-3820.201051 shu

Citation: ZHAO Dan, LI Na, XU Yan-Jie, HUANG Jing-Jing, CHEN Chuan-Xia. Colorimetric Sensing of Cyanide Based on Inhibition of Peroxidase-like Activity of Cysteamine-modified Gold Nanoparticles[J]. Chinese Journal of Analytical Chemistry, 2020, 48(7): 889-895. doi: 10.19756/j.issn.0253-3820.201051 shu

基于对半胱胺保护的金纳米粒子催化活性的抑制检测氰根离子

赵丹 ^1, ,
李娜 ^1, ,
许艳杰 ^1, ,
黄晶晶 ^1, ,
陈传霞 ^2,

1.
洛阳理工学院环境工程与化学学院, 洛阳 471023;
2.
济南大学材料科学与工程学院, 济南 250022
基金项目:
本文系河南省科技攻关计划项目（No.192102310405）、国家自然科学基金项目（No.21904048）、洛阳理工学院高层次人才启动项目（No.2017BZ16）和河南省教育厅重点项目（Nos.19A150002，20B150015，20B150016）资助

摘要: 利用氰根离子（CN^-）对带正电荷的半胱胺保护的金纳米粒子（cyst-AuNPs）过氧化物模拟酶催化活性的抑制，建立了一种简单、灵敏测定CN^-的比色传感方法。Cyst-AuNPs可催化过氧化氢（H₂O₂）氧化四甲基联苯胺（TMB），生成蓝色的oxTMB，最大吸收波长为652 nm。由于CN^-对cyst-AuNPs的蚀刻作用，cyst-AuNPs的催化活性减弱，导致生成的oxTMB量变少，溶液颜色变浅，652 nm处的吸光度下降，而颜色变浅和吸光度下降的程度与CN^-的浓度直接相关。基于催化体系的放大效应，此比色方法的灵敏度高，在最佳条件下，检测CN^-的线性范围为2~10 μmol/L和10~140 μmol/L，检出限为0.33 μmol/L（S/N=3）。本方法不需要对金纳米粒子表面进行修饰，具有操作简单、成本低、结果可视化等优点，具有潜在的实际应用价值。

关键词:

English

Colorimetric Sensing of Cyanide Based on Inhibition of Peroxidase-like Activity of Cysteamine-modified Gold Nanoparticles

1.
Department of Environmental Engineering and Chemistry, Luoyang Institute of Science and Technology, Luoyang 471023, China;
2.
School of Materials Science and Engineering, University of Jinan, Jinan 250022, China
Received Date: 19 January 2020
Revised Date: 27 April 2020
Fund Project:
This work was supported by the Project of Science and Technology Department (No. 192102310405), the National Natural Science Foundation of China (No. 21904048), the Scientific Research Foundation of Luoyang Institute of Science and Technology (No. 2017BZ16), the Project of Science and Technology Department (No. 192102310405) and the Key Project of the Education Department of Henan Province, China (Nos. 19A150002, 20B150015, 20B150016).

Abstract: A simple, sensitive and label-free colorimetric sensor for cyanide (CN^-) was developed based on analyte-induced shielding of peroxidase-like activity of cysteamine-capped gold nanoparticles (cyst-AuNPs). The cyst-AuNPs could catalyze the H₂O₂-mediated oxidation of 3,3',5,5'-tetramethylbenzidine (TMB), producing blue oxidized TMB (oxTMB) with a maximum absorption at 652 nm. With the addition of cyanide, AuNPs could be efficiently oxidized by the dissolved oxygen under alkaline conditions to form soluble and colorless complex, Au(CN)₂^-, which reduced the catalytic ability of AuNPs, thus producing less oxTMB. On the basis of this, both color and absorption at 652 nm decreased corresponding to the concentration of CN^-. Due to the signal amplification effect of the catalytic reaction, a detection limit as low as 0.33 μmol/L was obtained, which was much lower than the maximum level (1.9 μmol/L) of CN^- in drinking water permitted by the World Health Organization. Furthermore, the as-proposed sensor required neither surface modification of nanoparticles nor complex operations. These advantages make this sensor a powerful protocol for the quantitative detection of cyanide in water samples with satisfactory results.

Key words:

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沈阳化工大学材料科学与工程学院沈阳 110142

基于对半胱胺保护的金纳米粒子催化活性的抑制检测氰根离子

English

Colorimetric Sensing of Cyanide Based on Inhibition of Peroxidase-like Activity of Cysteamine-modified Gold Nanoparticles

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

基于对半胱胺保护的金纳米粒子催化活性的抑制检测氰根离子

English

Colorimetric Sensing of Cyanide Based on Inhibition of Peroxidase-like Activity of Cysteamine-modified Gold Nanoparticles

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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