核酸适配体亲和柱净化-高效液相色谱法测定莲子中黄曲霉毒素B<sub>1</sub>

引用本文: 赵颖, 王楠, 高华龙, 郭子轩, 陆安祥, 郭晓军, 卢静华, 栾云霞. 核酸适配体亲和柱净化-高效液相色谱法测定莲子中黄曲霉毒素B₁[J]. 分析化学, 2020, 48(5): 662-669. doi: 10.19756/j.issn.0253-3820.201030 shu

Citation: ZHAO Ying, WANG Nan, GAO Hua-Long, GUO Zi-Xuan, LU An-Xiang, GUO Xiao-Jun, LU Jing-Hua, LUAN Yun-Xia. Determination of Aflatoxin B₁ in Lotus Seed by High Performance Liquid Chromatography with Aptamer Affinity Column for Purification and Enrichment[J]. Chinese Journal of Analytical Chemistry, 2020, 48(5): 662-669. doi: 10.19756/j.issn.0253-3820.201030 shu

核酸适配体亲和柱净化-高效液相色谱法测定莲子中黄曲霉毒素B₁

1.
锦州医科大学, 药学院, 锦州 121001;
2.
北京农业质量标准与检测技术研究中心, 农业农村部农产品质量安全风险评估实验室(北京), 农产品产地环境监测北京市重点实验室, 北京 100097;
3.
首都师范大学, 北京 100097
基金项目:
本文系北京市农林科学院科技创新专项建设基金项目（No.KJCX20170401）和青年基金项目（No.QNJJ201903）资助

摘要: 以特异性的核酸适配体作为识别元件，制备了黄曲霉毒素B₁ （AFB₁）的适配体亲和柱（AAC），用于AFB₁的特异性识别和富集。对AAC的载样液中有机溶剂含量、载样液的体积和淋洗体积等条件进行了优化。此AAC吸附AFB₁的柱容量达到（334.6±18.2） ng，且AAC可重复使用20次。莲子样品经AAC净化富集后，用高效液相色谱-光化学衍生荧光法检测，外标法定量，AFB₁检出限达到0.05 ng/mL，回收率为91.8%~108.6%。本方法为食品、农产品和中药等复杂基质中痕量毒素的提取富集和分析提供了新的参考，具有良好的应用前景。

关键词:

English

Determination of Aflatoxin B₁ in Lotus Seed by High Performance Liquid Chromatography with Aptamer Affinity Column for Purification and Enrichment

1.
College of Pharmacy, Jinzhou Medical University, Jinzhou 121001, China;
2.
Beijing Research Center for Agricultural Standards and Testing, Agricultural Product Quality and Safety Risk Assessment Laboratory of the Department of Agriculture (Beijing), Beijing Municipal Key Laboratory of Agriculture Environment Monitoring, Beijing 100097, China;
3.
Capital Normal University, Beijing 100097, China
Received Date: 11 January 2020
Revised Date: 06 March 2020
Fund Project:
This work was supported by the Science and Technology Innovation Special Construction Funded Program of Beijing Academy of Agriculture and Forestry Sciences (No.KJCX20170401) and the Beijing Agricultural Forestry Academy Youth Foundation (No. QNJJ201903).

Abstract: An aptamer affinity column (AAC) was applied to specific identification and enrichment of aflatoxin B₁ (AFB₁). Aptamer was used as a novel recognition element for the high affinity and strong specificity of target. By optimizing the loading, washing and eluting conditions of AFB₁ in the sample, AFB₁ aptamer affinity column exhibited a good sample capacity (334.6±18.2) ng. The content of AFB₁ lotus seeds was detected by photo chemical reactor for enhanced high-performance liquid chromatography combined with photo chemical reactor for enhanced detection and fluorescence detection (HPLC-PCD-FLD) and AAC clean-up, with LOD of 0.05 ng/mL and excellent recoveries of 91.8%-108.6%. Meanwhile, the AAC could be reused at least twenty times. This study provides a new method for the enrichment and purification of trace toxins in complex matrix such as food, agricultural products and traditional Chinese medicines, and has a good application prospect.

Key words:

Aptamer affinity column
/ Aflatoxin B₁
/ Solid-phase extraction
/ N-Hydroxy succinimide-activated sepharose
/ Lotus seed

1. [1]
  XIE Yan-Jun, YANG Ying, KONG Wei-Jun, YANG Shi-Hai, YANG Mei-Hua. Chinese J. Anal. Chem.,2015,43(4): 618-628 谢艳君, 杨英, 孔维军, 杨世海, 杨美华.分析化学,2015,43(4): 618-628
2. [2]
  Shim W B, Mun H, Joung H A, Ofori J A, Chung D H, Kim M G. Food Control,2014,36(1): 30-35[LM]
3. [3]
  HOU Lei-Lei, CHAI Mei-Mei, MI Lin-Feng, JIA Li, AN Quan, ZHANG Dong-Dong. Journal of Food Safety & Quality,2018,9(11): 2631-2635 侯磊磊, 柴梅梅, 米林峰, 加力, 安全, 张东东.食品安全质量检测学报,2018,9(11): 2631-2635
4. [4]
  Ma X Y, Wang W F, Chen X J, Xia Y, Wu S J, Duan N, Wang Z P. Eur. Food Res.Technol.,2014,238(6): 919-925
5. [5]
  Chen L, Wen F, Li M, Guo X D, Li S L, Zheng N, Wang J Q. Food Chem.,2017,215(15): 377-382
6. [6]
  Sedova I, Kiseleva M, Tutelyan V. Toxins,2018,10(11): 444
7. [7]
  LUO Xiao-Rong, LI Ren-Zhao, FU Gang-Jian, HU Ri-Sheng, HUANG Gao-Ke, GE Xiao-Fei. Modern Food,2017,10(19): 115-119 罗小荣, 李人趙, 付刚建, 胡日升, 黄高科, 葛孝菲.现代食品,2017,10(19): 115-119
8. [8]
  Chen Z, Liu T, Tang J J, Zheng Z J, Wang H M, Shao Q, Chen G L, Li Z X, Chen Y Q, Zhu J W, Feng T. Environ. Sci. Pollut. Res.,2018,25(12): 11854-11866
9. [9]
  Wei F, Liu X, Liao X, Shi L, Zhang S, Lu J, Zhou L, Kong W. J. Pharm. Pharmacol.,2019,71(7): 1172-1183
10. [10]
  CHU Xiao-Feng, DOU Xiao-Wen, KONG Wei-Jun, YANG Mei-Hua, ZHAO Chong, ZHAO Ming, OUYANG Zhen. China Journal of Chinese Materia Medica,2015,40(4): 704-709 褚先锋, 豆小文, 孔维军, 杨美华, 赵翀, 赵明, 欧阳臻.中国中药杂志,2015,40(4): 704-709
11. [11]
  LIU Shu-Yu, QIU Feng, YANG Mei-Hua. China Journal of Chinese Materia Medica, 2012,37(3): 305-309 刘书宇, 仇峰, 杨美华.中国中药杂志,2012,37(3): 305-309
12. [12]
  Liu H M, Lu A X, Fu H L, Li B R, Yang M H, Wang J H, Luan Y X. Microchim. Acta,2018,185(7): UNSP326
13. [13]
  Sun C Y, Su R F, Bie J X, Sun H J, Qiao S N, Ma X Y, Sun R, Zhang T H. Dyes Pigments, 2017,149: 867-875
14. [14]
  Yang X H, Kong W J, Hu Y C, Yang M H, Huang L Q, Zhao M, Ouyang Z. J. Sep. Sci.,2014,37(7): 853-860
15. [15]
  LUO Ding-Qiang, HUANG Yan, FAN Bao-Juan, QIAO Rong-Xia. Anhui Medical and Pharmaceutical Journal,2014,18(1): 41-44 罗定强, 黄艳, 樊宝娟, 乔蓉霞.安徽医药,2014,18(1): 41-44
16. [16]
  Ferreira C S M, Papamichael K, Guilbault G, Schwarzacher T, Gariepy J. Missailidis S. Anal. Bioanal. Chem.,2008,390(4): 1039-1050
17. [17]
  Xiang L, Wu H L, Cui Z X, Tang J S. Anal. Lett.,2019,52(12): 1966-1975
18. [18]
  Ono E S Y, Silva M D, Ribeiro R M R, Ono M A, Hayashi L, Garcia G T, Hirooka E Y. Brazilian Arch. Bio. Tech.,2010,53(3): 687-692
19. [19]
  LI Xi-Fen, MAO Wen-Wen, ZHANG Ya-Qin, FENG Hong, XU Hong-Liang, LI Yan-Chuan, CAI Ying-Ying, ZHANG Lan-Lan. Modern Chinese Medicine,2018,20(8): 968-974 李细芬, 毛雯雯, 张雅琴, 冯红, 许宏亮, 李彦川, 蔡莹瀛, 张兰兰.中国现代中药,2018,20(8): 968-974
20. [20]
  Nian Y, Wang H, Ying G, Yang M, Wang Z, Kong W, Yang S. J. Pharm. Pharmacol.,2018,70(2): 278-288
21. [21]
  Setlem K, Mondal B, Ramlal S, Kingston J. Front. Microbiol.,2016,7: 1909
22. [22]
  ZHANG Hong-Bo, JIN Zhi-Min, GAO Zhi-Hui, ZHENG Yu-Shan. Food Safety Guide,2019,(1): 70-73 张宏博, 靳志敏, 高智慧, 郑玉山.食品安全导刊,2019,(1): 70-73
23. [23]
  Liu H M, Luan Y X, Lu A X, Li B R, Yang M H, Wang J H. Microchim. Acta,2018,185(1): NUSP71
24. [24]
  Song K M, Seonghwan L, Changill B. Sensors,2012,12(1): 612-631
25. [25]
  Li Y P, Sun L L, Zhao Q. Anal. Bioanal. Chem.,2018,410(24): 6269-6277
26. [26]
  Pichon V, Brothier F, Combés A. Anal. Bioanal. Chem.,2015,407(3): 681-698
27. [27]
  Madru B, Chapuis-Hugon F, Pichon V. Talanta,2011,85(1): 616-624
28. [28]
  De Girolamo A, Mckeague M, Miller J D, DeRosa M C, Visconti A. Food Chem., 2011, 127(3): 1378-1384
29. [29]
  Wallukat G, Haberland A, Berg S, Schulz A, Freyse E J, Dahmen C, Kage A, Dandel M, Vetter R, Salzsieder E, Kreutz R, Schimke I. Circ. J.,2012,76(10): 2449-2455
30. [30]
  Leichner C, Wulz P, Baus R A. Mol. Pharmaceut.,2019,16(3): 1211-1219
31. [31]
  Le Chryseis L, Cruz-Aguado J A, Penner G A. USA patent PTC/CA2010/001292,2012

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沈阳化工大学材料科学与工程学院沈阳 110142

核酸适配体亲和柱净化-高效液相色谱法测定莲子中黄曲霉毒素B₁

English

Determination of Aflatoxin B₁ in Lotus Seed by High Performance Liquid Chromatography with Aptamer Affinity Column for Purification and Enrichment

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

核酸适配体亲和柱净化-高效液相色谱法测定莲子中黄曲霉毒素B1

English

Determination of Aflatoxin B1 in Lotus Seed by High Performance Liquid Chromatography with Aptamer Affinity Column for Purification and Enrichment

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

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CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

核酸适配体亲和柱净化-高效液相色谱法测定莲子中黄曲霉毒素B₁

Determination of Aflatoxin B₁ in Lotus Seed by High Performance Liquid Chromatography with Aptamer Affinity Column for Purification and Enrichment

CCS Chemistry：颜徐州、鲍哲南：你的皮肤可能是一片SPMs