蛋白质的核酸适配体筛选及应用的研究进展

引用本文: 赵丽萍, 杨歌, 张小敏, 屈锋. 蛋白质的核酸适配体筛选及应用的研究进展[J]. 分析化学, 2020, 48(5): 560-572. doi: 10.19756/j.issn.0253-3820.201024 shu

Citation: ZHAO Li-Ping, YANG Ge, ZHANG Xiao-Min, QU Feng. Development of Apatmer Screening Against Proteins and Its Applications[J]. Chinese Journal of Analytical Chemistry, 2020, 48(5): 560-572. doi: 10.19756/j.issn.0253-3820.201024 shu

蛋白质的核酸适配体筛选及应用的研究进展

北京理工大学生命学院, "分子医学与生物诊疗"工业和信息化部重点实验室, 北京 100081
基金项目:
本文系国家自然科学基金项目（Nos.21675012，21874010，21827810）资助

摘要: 核酸适配体是通过指数富集配体系统进化（SELEX）筛选方法获得的，能够与靶标分子高亲和力和特异性结合的单链DNA或RNA。蛋白质是一种非常重要的生物功能大分子，迄今为止，已经开发了许多SELEX技术，用于蛋白质的适配体的筛选。目前，适配体的筛选优化方法主要集中在提高筛选效率、降低筛选成本，以及提升适配体性能等方面，从而获得可与靶标分子以高亲和力和高特异性结合的适配体。适配体与靶标分子亲和力的表征是关键的筛选步骤，用于判定所筛选的适配体是否可以满足后续应用需求。本文归纳总结了2016年以来蛋白质靶标的适配体筛选的研究进展，对核酸适配体筛选过程中有关核酸库的优化方法和筛选方法的改进、新筛选方法的开发、核酸适配体的应用等方面的研究进行了评述。

关键词:

English

Development of Apatmer Screening Against Proteins and Its Applications

School of Life Science, Beijing Institute of Technology, Key Laboratory of Molecular Medicine and Biological Diagnosis, Ministry of Industry and Information Technology, Beijing 100081, China
Received Date: 09 January 2020
Revised Date: 25 February 2020
Fund Project:
This work was supported by the National Natural Science Foundation of China (Nos. 21675012, 21874010, 21827810).

Abstract: Aptamers, short single stranded DNA or RNA, are selected through systematic evolution of ligands by exponential enrichment (SELEX), which can bind with target molecules with high affinity and high specificity. Proteins are vital functional macromolecules. Many SELEX techniques have been developed for screening of aptamers against proteins. In recent years, in order to obtain aptamers with high affinity and specificity, many SELEX methods have been developed to enhance selection efficiency, reduce cost and improve aptamer properties. Affinity characterization is also the key procedure in aptamer selection, which demonstrates if the obtained aptamer can satisfy the application requirement. In this review, the progress in aptamer screening for protein targets were summarized, and the optimization methods of nucleic acid library, improvement and development of screening methods and application of aptamers against proteins were mainly emphasized.

Key words:

Aptamer
/ Protein
/ Selection
/ Systematic evolution of ligands by exponential enrichment
/ Review

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蛋白质的核酸适配体筛选及应用的研究进展

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Development of Apatmer Screening Against Proteins and Its Applications

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