微流控芯片液滴数字化分析用于快速定量检测细菌

何浩延 黄恩奇 黎柱均 舒博文 徐邦牢 刘大渔

引用本文: 何浩延,  黄恩奇,  黎柱均,  舒博文,  徐邦牢,  刘大渔. 微流控芯片液滴数字化分析用于快速定量检测细菌[J]. 分析化学, 2020, 48(7): 855-862. doi: 10.19756/j.issn.0253-3820.201022 shu
Citation:  HE Hao-Yan,  HUANG En-Qi,  LI Zhu-Jun,  SHU Bo-Wen,  XU Bang-Lao,  LIU Da-Yu. Droplet Digital Microfluidic Assay for Rapid Bacterium Quantitation[J]. Chinese Journal of Analytical Chemistry, 2020, 48(7): 855-862. doi: 10.19756/j.issn.0253-3820.201022 shu

微流控芯片液滴数字化分析用于快速定量检测细菌

  • 基金项目:

    本文系国家自然科学基金项目(Nos.81871726,21804044)和广州市科创委科学研究专项(Nos.201707010277,201904010237,201904010413)资助

摘要: 现有细菌定量检测多依赖专业实验室,检测周期较长。针对此问题,本研究基于微流控芯片液滴数字化分析,建立了一种细菌定量检测方法。采用具有平行液滴分析单元的微流控芯片,其特点在于使用了注射器真空驱动液滴产生方法。借助刃天青显色反应引起的荧光强度改变,可指示液滴内活性细菌的存在。通过计算细菌阳性液滴的比例,采用泊松分布算法,计算出原始样品中的细菌密度。实验结果表明,本方法可在3.5 h内完成细菌定量分析,动态检测范围为105~108 CFU/mL,相对标准偏差(RSD)低于5%。本方法具有操作简便和分析速度快的优势,有望广泛用于细菌快速定量检测。

English


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  • 收稿日期:  2020-01-09
  • 修回日期:  2020-02-24
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