基于单颗粒电感耦合等离子体质谱技术的金纳米颗粒准确测定和表征

引用本文: 巢静波, 王静如, 张靖其. 基于单颗粒电感耦合等离子体质谱技术的金纳米颗粒准确测定和表征[J]. 分析化学, 2020, 48(7): 946-954. doi: 10.19756/j.issn.0253-3820.191740 shu

Citation: CHAO Jing-Bo, WANG Jing-Ru, ZHANG Jing-Qi. Accurate Determination and Characterization of Gold Nanoparticles Based on Single Particle-Inductively Coupled Plasma-Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, 2020, 48(7): 946-954. doi: 10.19756/j.issn.0253-3820.191740 shu

基于单颗粒电感耦合等离子体质谱技术的金纳米颗粒准确测定和表征

1.
中国计量科学研究院化学所, 北京 100029;
2.
北京化工大学化学工程学院, 北京 100029
基金项目:
本文系国家重点研发计划（No.2017YFF0205801）和中国计量科学研究院基本科研业务费（21-AKYS1609）资助

摘要: 建立了基于单颗粒电感耦合等离子体质谱（SP-ICP-MS）技术的金纳米颗粒（AuNPs）数量浓度和粒径的准确测定方法，考察了传输效率计算方式、驻留时间、稀释试剂、进样方式等对测定结果的影响。传输效率对结果准确度影响较大，应根据测量目标参数选择传输效率计算方式，并在测定过程中保持传输效率的稳定，利用校准标准对其进行校正。驻留时间的选择直接影响结果的精确程度，应根据样品类型、粒径等性质慎重选择数据处理模式。当驻留时间为0.1 ms时，检测30 nm和60 nm AuNPs的线性范围分别为2~40 pg/g和10~200 pg/g，线性相关系数大于0.9998，数量浓度测定结果的相对标准偏差小于3.80%，颗粒数量浓度和粒径的检出限分别为110 NPs/g和9 nm。对合成的4种AuNPs和国际比对样品分别进行了测定，国际比对样品颗粒数量浓度结果与比对参考值偏差<5%，测定结果等效一致，扩展不确定度<15%（k=2），进一步验证了本方法的准确度和适用性。

关键词:

English

Accurate Determination and Characterization of Gold Nanoparticles Based on Single Particle-Inductively Coupled Plasma-Mass Spectrometry

1.
Division of Chemical Metrology and Analytical Science, National Institute of Metrology, Beijing 100029, China;
2.
Chemical Engineering School, Beijing University of Chemical Technology, Beijing 100029, China
Received Date: 14 December 2019
Revised Date: 08 May 2020
Fund Project:
This work was supported by the National Key Research and Development Project of China (No.2017YFF0205801) and the NIM Fundamental Research Program (No. 21-AKYS1609).

Abstract: An accurate determination method for gold nanoparticle number concentration and size was developed based on single particle-inductively coupled plasma-mass spectrometry (SP-ICP-MS). Influence factors such as different calculation methods of transport efficiency, dwell time, dispersing agent and injection mode were investigated and optimized. The transport efficiency had a great impact on the accuracy of the measurement result, and it should be selected according to the measured target parameters. It was important to keep the stability of the transport efficiency and used the calibration standard calibrating during the measurement process. Dwell time directly affected the precision of result, and the data processing mode should be selected carefully according to the sample type, particle size and other properties. When the dwell time was 0.1 ms, for 30 nm and 60 nm gold nanoparticles, the linear ranges were 2-40 pg/g and 10-200 pg/g, respectively, the linear correlation coefficients were greater than 0.9998. The relative standard deviation of particle number concentration was less than 3.80%. The detection limits of particle number concentration and particle size were 110 NPs/g and 9 nm, respectively. Four kinds of synthesized gold nanoparticles and CCQM-P194 international pilot study sample were determined by the established method. The diviation of number concentration in pilot study from the reference values of the key comparison was less than 5%. The results were equivalent and the expanded uncertainty was less than 15% (k=2), which further verified the accuracy and applicability of the method.

Key words:

Particle number concentration
/ Particle size
/ Gold nanoparticles
/ Single particle inductively coupled plasma mass spectrometry

1. [1]
  CPI Home. An Inventory Nanotechnology-based Consuner Products Introduced on the Market, http://www.nanotechproject.org/cpi/,2014
2. [2]
  Alkilany A M, Murphy C J. J. Nanopart. Res.,2010,12(7):2313-2333
3. [3]
  Balasubramanian S K, Jittiwat J, Manikandan J, Ong C N, Yu L E, Ong W Y. Biomaterials,2010,31(8):2034-2042
4. [4]
  Barreto A, Luis L G, Pinto E, Almeida A, Paiga P, Santos L, Delerue-Matos C, Trindade T, Soares A, Hylland K, Loureiro S, Oliveira M. Chemosphere,2019,220:11-19
5. [5]
  Klaine S J, Alvarez P J J. Environ. Toxicol. Chem., 2008,27(9):1825-1851
6. [6]
  2011/696/EU, Commission Recommendation of 18 October 2011 on the Definition of Nanomaterial. Official Journal of the European Union
7. [7]
  Regulation (EC) No 1223/2009 of the European Parliament and of the Council of 30 November 2009 on Cosmetic Products, Official Journal of the European Union
8. [8]
  Meermann B, Nischwitz V. J. Anal. At. Spectrom.,2018,33(9):1432-1468
9. [9]
  WANG Juan, YAO Ji-Jun. Environ. Chem.,2017,36(12):2749-2750 王娟, 姚继军.环境化学,2017,36(12):2749-2750
10. [10]
  LUO Rui-Ping, ZHENG Ling-Na, LI Liang, WANG Juan, FENG Wei-Yue, YU Xiang-Hua, WANG Meng. Chinese J. Anal. Chem.,2018,46(6):925-930 罗瑞平, 郑令娜, 李亮, 王娟, 丰伟悦, 喻湘华, 王萌.分析化学,2018,46(6):925-930
11. [11]
  Laborda F, Jiménez-Lamana J, Bolea E, Castillo J R. J. Anal. At. Spectrom.,2011,26(7):1362-1371
12. [12]
  Costa-Fernández J M, Menéndez-Miranda M, Bouzas-Ramos D, Encinar J R, Sanz-Medel A. TrAC-Trends Anal. Chem.,2016,84:139-148
13. [13]
  Degueldre C, Favarger P Y. Colloids Surf. A,2003,217(1-3):137-142
14. [14]
  Donovan A R, Adams C D, Ma Y, Stephan C, Eichholz T, Shi H. Chemosphere, 2016,144:148-153
15. [15]
  Lu P J, Fang S W, Cheng W L, Huang S C, Huang M C, Cheng H F. J. Food Drug Anal.,2018,26(3):1192-1200
16. [16]
  Stefan W, Ruud P, Katrin L, Ringo G, Thomas P J L. Anal. Bioanal. Chem.,2017,409:4839-4848
17. [17]
  Thomas P J L, Ruud P, Stefan W. Anal. Bioanal. Chem.,2014,406:3835-3843
18. [18]
  Antonio R M B, Elijah J R, Antonio P, Michael R W. Anal. Chem.,2015,87:8809-8817
19. [19]
  Bastús N G, Comenge J, Puntes V. Langmuir,2011,27(17):11098-11105
20. [20]
  Pace H E, Rogers N J, Jarolimek C, Coleman V A, Higgins C P, Ranville J F. Anal. Chem.,2011, 83(24):9361-9369
21. [21]
  YANG Yuan, LONG Chen-Lu, YANG Zhao-Guang, LI Hai-Pu, WANG Qiang. Chinese J. Anal. Chem., 2014,42(11):1553-1560 杨远, 龙晨璐, 杨兆光, 李海普, 王强.分析化学,2014,42(11):1553-1560
22. [22]
  Montaño M D, Olesik J W, Barber A G, Challis K, Ranville J F. Anal. Bioanal. Chem., 2016,408(19):5053-5074
23. [23]
  Abad-Alvaro I, Pena-Vazquez E, Bolea E, Bermejo-Barrera P, Castillo J R, Laborda F. Anal. Bioanal. Chem., 2016,408(19):5089-5097
24. [24]
  Mitrano D M, Lesher E K, Bednar A, Monserud J, Higgins C P, Ranville J F. Environ. Toxicol. Chem., 2012,31(1):115-121
25. [25]
  Laborda F, Jiménez-Lamana J, Bolea E, Castillo J R. J. Anal. At. Spectrom., 2013,28(8):1220-1232

扫一扫看文章

计量

PDF下载量: 11
文章访问数: 1500
HTML全文浏览量: 177

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

基于单颗粒电感耦合等离子体质谱技术的金纳米颗粒准确测定和表征

English

Accurate Determination and Characterization of Gold Nanoparticles Based on Single Particle-Inductively Coupled Plasma-Mass Spectrometry

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南：你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

计量

通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

基于单颗粒电感耦合等离子体质谱技术的金纳米颗粒准确测定和表征

English

Accurate Determination and Characterization of Gold Nanoparticles Based on Single Particle-Inductively Coupled Plasma-Mass Spectrometry

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

计量

文章相关

通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

CCS Chemistry：颜徐州、鲍哲南：你的皮肤可能是一片SPMs