纳米金-适配体比色传感法检测玉米油中黄曲霉毒素B<sub>1</sub>

引用本文: 杨妍, 尹盈爱, 董益阳. 纳米金-适配体比色传感法检测玉米油中黄曲霉毒素B₁[J]. 分析化学, 2020, 48(5): 676-684. doi: 10.19756/j.issn.0253-3820.191728 shu

Citation: YANG Yan, YIN Ying-Ai, DONG Yi-Yang. Detection of Aflatoxin B₁ in Corn Oil by Colorimetric Gold Nanoparticles-Aptasensor[J]. Chinese Journal of Analytical Chemistry, 2020, 48(5): 676-684. doi: 10.19756/j.issn.0253-3820.191728 shu

纳米金-适配体比色传感法检测玉米油中黄曲霉毒素B₁

北京化工大学生命科学与技术学院, 北京 100029
基金项目:
本文系"十三五"国家重点研发计划NQI专项项目（No.2016YFF0203703）资助

摘要: 黄曲霉毒素B₁（Aflatoxin B₁，AFB₁）是真菌或霉菌产生的有毒代谢物，通过污染农作物和食品从而对人体健康造成严重威胁。本研究建立了纳米金（AuNPs）-适配体比色传感法，采用不同序列长度的AFB₁适配体检测玉米油中AFB₁。通过优化适配体浓度、NaCl浓度和体系反应温度，进一步提高体系检测灵敏度。在最佳条件下，50（B50）和80（A80）个碱基长度的适配体建立的AuNPs-适配体比色传感法的检出限分别为13.55和10.56 ng/mL，线性范围均为20~1000 ng/mL，且选择性良好。基于B50和A80适配体比色传感法检测玉米油中AFB₁的加标回收率分别为102.4%~104.9%和98.1%~109.1%。结果表明，基于B50和A80建立的AuNPs比色传感法均可用于玉米油中AFB₁特异性鉴定。本研究为现场快速检测和高效筛查食品中AFB₁污染提供了技术支持，为开发针对其它靶标的适配体传感检测方法奠定了基础。

关键词:

English

Detection of Aflatoxin B₁ in Corn Oil by Colorimetric Gold Nanoparticles-Aptasensor

College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China
Received Date: 10 December 2019
Revised Date: 14 April 2020
Fund Project:
This work was supported by the National Key Research and Development Program of China (No.2016YFF0203703).

Abstract: Aflatoxin B₁ (AFB₁) is a toxic metabolite produced by fungi or molds, which can cause serious harm to human health. Based on the classic gold nanoparticles (AuNPs)-based colorimetric method, an AuNPs-aptasensor applicable for two different lengths of AFB₁ aptamers determining AFB₁ in corn oil was established in this study. The sensitivity of the AuNPs-aptasensor was further improved by optimizing the concentration of aptamer and NaCl and the reaction temperature. Under the optimal conditions, the limits of detection of AuNPs-aptasensor based on 50 (B50) and 80 (A80) base aptamers were 13.55 ng/mL and 10.56 ng/mL, respectively, with the linear range of 20-1000 ng/mL. Moreover, the AuNPs-aptasensor had good selectivity and the recoveries were 102.4%-104.9% and 98.1%-109.1%, respectively. The results indicated that AuNPs-aptasensor could be utilized as an efficient tool for AFB₁ identification in corn oil sample. The successful development of AuNPs-aptasensor provided a powerful support for rapid on-site detection and efficient screening of AFB₁ in food and showed the potential for construction of biosensors based on other targets and aptamers.

Key words:

Aflatoxin B₁
/ Aptamer
/ Colorimetric gold nanoparticles-aptasensor
/ Corn oil

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纳米金-适配体比色传感法检测玉米油中黄曲霉毒素B1

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Detection of Aflatoxin B1 in Corn Oil by Colorimetric Gold Nanoparticles-Aptasensor

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纳米金-适配体比色传感法检测玉米油中黄曲霉毒素B₁

Detection of Aflatoxin B₁ in Corn Oil by Colorimetric Gold Nanoparticles-Aptasensor