纳米金-适配体比色传感法检测玉米油中黄曲霉毒素B1

杨妍 尹盈爱 董益阳

引用本文: 杨妍,  尹盈爱,  董益阳. 纳米金-适配体比色传感法检测玉米油中黄曲霉毒素B1[J]. 分析化学, 2020, 48(5): 676-684. doi: 10.19756/j.issn.0253-3820.191728 shu
Citation:  YANG Yan,  YIN Ying-Ai,  DONG Yi-Yang. Detection of Aflatoxin B1 in Corn Oil by Colorimetric Gold Nanoparticles-Aptasensor[J]. Chinese Journal of Analytical Chemistry, 2020, 48(5): 676-684. doi: 10.19756/j.issn.0253-3820.191728 shu

纳米金-适配体比色传感法检测玉米油中黄曲霉毒素B1

  • 基金项目:

    本文系"十三五"国家重点研发计划NQI专项项目(No.2016YFF0203703)资助

摘要: 黄曲霉毒素B1(Aflatoxin B1,AFB1)是真菌或霉菌产生的有毒代谢物,通过污染农作物和食品从而对人体健康造成严重威胁。本研究建立了纳米金(AuNPs)-适配体比色传感法,采用不同序列长度的AFB1适配体检测玉米油中AFB1。通过优化适配体浓度、NaCl浓度和体系反应温度,进一步提高体系检测灵敏度。在最佳条件下,50(B50)和80(A80)个碱基长度的适配体建立的AuNPs-适配体比色传感法的检出限分别为13.55和10.56 ng/mL,线性范围均为20~1000 ng/mL,且选择性良好。基于B50和A80适配体比色传感法检测玉米油中AFB1的加标回收率分别为102.4%~104.9%和98.1%~109.1%。结果表明,基于B50和A80建立的AuNPs比色传感法均可用于玉米油中AFB1特异性鉴定。本研究为现场快速检测和高效筛查食品中AFB1污染提供了技术支持,为开发针对其它靶标的适配体传感检测方法奠定了基础。

English


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文章相关
  • 收稿日期:  2019-12-10
  • 修回日期:  2020-04-14
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