基于聚苯胺纳米孔的DNA分析

引用本文: 赵怡, 石晓雨, 孙红, 姚付军, 亢晓峰. 基于聚苯胺纳米孔的DNA分析[J]. 分析化学, 2020, 48(3): 332-338. doi: 10.19756/j.issn.0253-3820.191697 shu

Citation: ZHAO Yi, SHI Xiao-Yu, SUN Hong, YAO Fu-Jun, KANG Xiao-Feng. Polyaniline Nanopores for DNA Analysis[J]. Chinese Journal of Analytical Chemistry, 2020, 48(3): 332-338. doi: 10.19756/j.issn.0253-3820.191697 shu

基于聚苯胺纳米孔的DNA分析

西北大学化学与材料科学学院合成与天然功能分子化学教育部重点实验室, 西安 710069
基金项目:
本文系国家自然科学基金项目（Nos.21327806，21874107）资助

摘要: 固态纳米孔作为传感元件已被广泛应用于蛋白和核酸分析。然而，极低的捕获效率及较快的穿孔速率限制了固体纳米孔传感器的灵敏度和分辨率。本研究制备了一种聚苯胺导电聚合物修饰的固态纳米孔，探究了ssDNA和dsDNA在其中的穿孔行为。研究表明，聚苯胺涂层与DNA间的静电相互作用能显著地将ssDNA的穿孔速率降低至48.2 μs/base；同时，通过采用LiCl作为电解质溶液，此聚苯胺纳米孔对ssDNA的捕获效率明显增加。进一步研究发现，基于阻塞电流幅度和穿孔时间的不同，聚苯胺纳米孔能够实现ssDNA与dsDNA的区分。研究结果表明，固态纳米孔的功能修饰能够有效调控DNA分子穿孔行为。制备的聚苯胺纳米孔有望作为一个单分子纳米器件应用于生物分子的分析和检测。

关键词:

English

Polyaniline Nanopores for DNA Analysis

Key Laboratory of Synthetic and Natural Functional Molecular Chemistry, College of Chemistry and Materials Science, Northwest University, Xi'an 710069, China
Received Date: 27 November 2019
Revised Date: 23 December 2019
Fund Project:
This work was supported by the National Natural Science Foundation of China (Nos. 21327806, 21874107)

Abstract: Solid state nanopores are widely used as sensing elements for protein and nucleic acid analysis. However, the extremely low capture rate and fast translocation speed limit the sensitivity and resolution of nanopore-based sensors. In this work, we prepared a polyaniline conductive polymer-modified solid nanopore and explored its application for studying the translocation behavior of ssDNA and dsDNA. The results demonstrated that the electrostatic interaction between the polyaniline and DNA significantly reduced the translocation rate of ssDNA to 48.2 μs/base; meanwhile, the capture efficiency of the polyaniline nanopore for ssDNA was significantly increased by using LiCl as the electrolyte solution. Additional experimental results revealed that polyaniline nanopores could distinguish ssDNA and dsDNA based on the difference in blockage amplitude and translocation time. The results showed that functional modification of solid state nanopore could regulate DNA translocation behavior effectively. In addition, the polyaniline nanopores were expected to be used as a single-molecule nanodevice for the analysis and detection of biomolecules.

Key words:

1. [1]
  Chen X, Roozbahani G M, Ye Z, Zhang Y, Ma R, Xiang J, Guan X. ACS Appl. Mater. Interfaces, 2018,10(14):11519-11528
2. [2]
  Gu Z, Ying Y L, Cao C, He P, Long Y T. Anal. Chem.,2015,87(2):907-913
3. [3]
  Lin Y, Ying Y L, Shi X, Liu S, Long Y T. Chem. Commun.,2017,53(84):11564-11567
4. [4]
  Kukwikila M, Howorka S. Anal. Chem.,2015,87(18):9149-9154
5. [5]
  Wang L, Yao F, Kang X. Anal. Chem.,2017,89(15):7958-7965
6. [6]
  Shi W, Friedman A K, Baker L A. Anal. Chem., 2017,89(1):157-188
7. [7]
  Roozbahani G M, Chen X, Zhang Y, Juarez O, Li D, Guan X. Anal. Chem.,2018,90(9):5938-5944
8. [8]
  Liu L, Fang Z, Zheng X, Xi D. ACS Sens.,2019,4(5):1323-1328
9. [9]
  Boersma A J, Brain K L, Hagan B. ACS Nano,2012,6(6):5304-5308
10. [10]
  Chavis A E, Brady K T, Hatmaker G A, Angevine C E, Kothalawala N, Dass A, Robertson J W F, Reiner J E. ACS Sens.,2017,2(9):1319-1328
11. [11]
  Zhou S, Wang L, Chen X, Guan X. ACS Sens.,2016,1(5):607-613
12. [12]
  Wang F, Zahid O K, Swain B, Parsonage D, Hollis T, Harvey S, Perrino F, Kohli R M, Taylor E W, Hall A R. Nano Lett.,2017,17(11):7110-7116
13. [13]
  Clamer M, Höfler L, Mikhailova E, Viero G, Bayley H. ACS Nano,2014,8(2):1364-1374
14. [14]
  Wanunu M, Morrison W, Rabin Y, Grosberg A Y, Meller A. Nat. Nanotechnol., 2010,5(2):160-165
15. [15]
  He Y, Tsutsui M, Scheicher R H, Bai F, Taniguchi M, Kawai T. ACS Nano,2013,7(1):538-546
16. [16]
  Fologea D, Uplinger J, Thomas B, McNabb D S, Li J. Nano Lett.,2005,5(9):1734-1737
17. [17]
  Wang Y, Yao F, Kang X. Anal. Chem., 2015,87(19):9991-9997
18. [18]
  Ren R, Leng C, Zhang S. Biosens. Bioelectron., 2010,25(9):2089-2094
19. [19]
  Chang H, Yuan Y, Shi N, Guan Y. Anal. Chem.,2007,79(13):5111-5115
20. [20]
  Li Z H, Hu X T, Shi J Y, Zou X B, Huang X W, Zhou X C, Tahir H E, Holmes M, Povey M. Biosens. Bioelectron.,2016,81:75-79
21. [21]
  LI Jian-Ping, PENG Tu-Zhi. Chin. J. Chem.,2010,20(10):1038-1043 李建平, 彭图治.中国化学, 2010,20(10):1038-1043
22. [22]
  Arora K, Prabhakar N, Chand S, Malhotra B D. Biosens. Bioelectron.,2007,23(5):613-620
23. [23]
  Gospodinova N, Terlemezyan L. Progs. Polym. Sci.,1998,23(8):1443-1484
24. [24]
  Siwy Z S. Adv. Funct. Mater., 2006,16(6):735-746
25. [25]
  Kowalczyk S W, Wells D B, Aksimentiev A, Dekker C. Nano Lett.,2012,12(2):1038-1044
26. [26]
  Wang D, Harrer S, Luan B, Stolovitzky G, Peng H, Afzali-Ardakani A. Sci. Rep., 2014,4:3985
27. [27]
  Plesa C, van Loo N, Ketterer P, Dietz H, Dekker C. Nano Lett.,2015,15(1):732-737
28. [28]
  Meller A, Nivon L, Brandin E, Golovchenko J, Branton D. Proc. Natl. Acad. Sci. USA,2000,97:1079-1084
29. [29]
  Hu Z L, Li M Y, Liu C, Ying Y L, Long Y T. Chem. Sci., 2019,10(2):354-358
30. [30]
  Piguet F, Discala F, Breton M F, Pelta J, Bacri L, Oukhaled A. J. Phys. Chem. Lett., 2014,5(24):4362-4367

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沈阳化工大学材料科学与工程学院沈阳 110142

基于聚苯胺纳米孔的DNA分析

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Polyaniline Nanopores for DNA Analysis

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