基于谷胱甘肽修饰的金纳米簇选择性检测铜离子

引用本文: 安春, 杜佩瑶, 张振, 卢小泉. 基于谷胱甘肽修饰的金纳米簇选择性检测铜离子[J]. 分析化学, 2020, 48(3): 355-362. doi: 10.19756/j.issn.0253-3820.191668 shu

Citation: AN Chun, DU Pei-Yao, ZHANG Zhen, LU Xiao-Quan. Rapid and Highly Selective Detection of Copper Ion with Glutathione-capped Gold Nanoclusters[J]. Chinese Journal of Analytical Chemistry, 2020, 48(3): 355-362. doi: 10.19756/j.issn.0253-3820.191668 shu

基于谷胱甘肽修饰的金纳米簇选择性检测铜离子

安春 ^1, ,
杜佩瑶 ^1, ,
张振 ^1, ,
卢小泉 ^1,2,

1.
天津市分子光电科学重点实验室, 天津大学理学院化学系, 天津 300072;
2.
甘肃省生物电化学与环境分析重点实验室, 西北师范大学化学化工学院, 兰州 730070
基金项目:
本文系国家自然科学基金项目（Nos.21575115，21705117）资助

摘要: 建立了一种基于谷胱甘肽（GSH）包裹的金纳米簇（AuNCs）高选择性检测水中和血清中铜离子（Cu²⁺）的方法。Cu²⁺与AuNCs配体上的氨基（-NH₂）和羧基（-COOH）发生配位作用，阻断配体-金属间或配体-金属-金属间的电荷转移，导致AuNCs的荧光猝灭； EDTA与Cu²⁺具有更强的配位作用，可将Cu²⁺从AuNCs表面移除，使AuNCs荧光恢复。本研究中，AuNCs发射红色荧光，避免了复杂生物基质背景荧光的干扰，在pH=5.5的条件下，可快速、灵敏、高选择性地检测Cu²⁺，检出限为23 nmol/L。血清和水样中Cu²⁺的加标回收率为96.2%~100.1%。本方法在药物分析、环境监测、临床诊断等方面具有广阔的应用前景。

关键词:

金纳米簇
/ 荧光
/ 铜离子
/ 检测

English

Rapid and Highly Selective Detection of Copper Ion with Glutathione-capped Gold Nanoclusters

1.
Tianjin Key Laboratory of Molecular Optoelectronic Science, Department of Chemistry, School of Science, Tianjin University, Tianjin 300072, China;
2.
Key Laboratory of Bioelectrochemistry&Environmental Analysis of Gansu Province, College of Chemistry&Chemical Engineering, Northwest Normal University, Lanzhou 730070, China
Received Date: 13 November 2019
Revised Date: 03 January 2020
Fund Project:
This work was supported by the National Natural Science Foundation of China (Nos. 21575115, 21705117)

Abstract: The gold nanoclusters (AuNCs) with red fluorescence were synthesized using glutathione (GSH) as protectants and reductants, which displayed high selectivity to Cu²⁺ in water and rat serum. Due to the coordination of Cu²⁺ with the capping ligand of AuNCs, ligand-metal charge transfer (LMCT) or ligand-metal-metal charge transfer (LMMCT) was blocked, and the fluorescence of the gold cluster was quenched, so Cu²⁺ could be selectively detected. Moreover, the gold cluster displayed ultrahigh selectivity and excellent sensitivity to Cu²⁺, which could shield 16 other interfering substances, with a detection limit of 23 nmol/L(S/N=3). In addition, the reliability of AuNCs was also verified by detection of Cu²⁺ in water samples and rat serum with satisfactory recoveries. This study provides a simple, fast and reliable method to detect Cu²⁺. The method is expected to be extended to the diagnosis of diseases, biological imaging, biomarker, water quality analysis, etc.

Key words:

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沈阳化工大学材料科学与工程学院沈阳 110142

基于谷胱甘肽修饰的金纳米簇选择性检测铜离子

English

Rapid and Highly Selective Detection of Copper Ion with Glutathione-capped Gold Nanoclusters

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