Citation: TU Yu, LI Wu-Shuang, LANG Wen-Cheng, MA Lin, WU Guo-Hua. Determination of γ-Aminobutyric Acid in Different Tea Samples by Triple Quadrupole Mass Spectrometry in Real Time[J]. Chinese Journal of Analytical Chemistry, ;2020, 48(9): 1252-1259. doi: 10.19756/j.issn.0253-3820.191665 shu

Determination of γ-Aminobutyric Acid in Different Tea Samples by Triple Quadrupole Mass Spectrometry in Real Time

TU Yu ^1, ,
LI Wu-Shuang ¹ ,
LANG Wen-Cheng ² ,
MA Lin ² ,
WU Guo-Hua ^1,2

1.
School of Environmental and Chemical Engineering, Jiangsu University of Science and Technology, Zhenjiang 212018, China;
2.
Laboratory of Risk Assessment for Sericultural Products and Edible Insects, College of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212018, China

Received Date: 11 November 2019
Revised Date: 29 June 2020

Fund Project: This work was supported by the Jiangsu Specially Appointed Professor Program (No. Sujiaoshi([2015]17).

Tea contains a variety of functional active substances such as γ-aminobutyric acid (GABA), which plays a good role in health care. Rapid identification of active components such as γ-aminobutyric acid in tea is conducive to improving the effective utilization of tea resources. In this work, qualitative and quantitative analysis of γ-aminobutyric acid in tea by direct analysis in real time (DART) ionization source coupled with triple quadrupole mass spectrometry was developed. The content of γ-aminobutyric acid in 10 kinds of tea samples was determined by this method. The results showed that the content of GABA in different tea samples were different, among which Jiuxiang green bud tea had the highest GABA content (227.2 μg/g), followed by Xinyang Maojian tea (161.2 μg/g), and Rizhao green tea had the lowest GABA content (75.6 μg/g). The test results are in good agreement with the results of the 2,4-dinitrofluorobenzene pre-column HPLC method. This method is simple and efficient, which can provide an effective method for rapidly screening tea varieties with high content of GABA. DART-MS method also has broad application in the fields of food active substance analysis, environmental organic pollutants, early screening of metabolic diseases and other fields.
- Tea,
- γ-Aminobutyric acid,
- Real time,
- Direct analysis,
- Triple quadrupole mass spectrometry,
- High performance liquid chromatography
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