Advanced Search

Citation: DENG Yan,  MA Yu-Chan,  WANG Min,  GU Men-Qiao,  CHI Kuan-Neng,  HU Rong,  YANG Yun-Hui. Ratio Type C-reactive Protein Immunosensor Based on Gold Nanoparticles/Fe-MIL-88-NH2[J]. Chinese Journal of Analytical Chemistry, ;2020, 48(4): 498-506. doi: 10.19756/j.issn.0253-3820.191662 shu

Ratio Type C-reactive Protein Immunosensor Based on Gold Nanoparticles/Fe-MIL-88-NH2

  • College of Chemistry and Chemical Engineering, Yunnan Normal University, Kunming 650500, China

  • Received Date: 11 November 2019
    Revised Date: 6 February 2020

    Fund Project: This work was supported by the National Natural Science Foundation of China (Nos. 21465026, 21865026)

  • A dual-signal proportional immunosensor for detection of C-reactive protein was developed using gold-nanoparticles (AuNPs) functionalized metal-organic frameworks (MOFs) material (AuNPs /Fe-MIL-88-NH2) as an immobilized matrix and signal unit. The octahedral Fe-MOFs can not only provide a larger effective surface area, but also increase the amount of immobilized biomolecules and promote the transport of electrons and ions, and also exhibit good electrical conductivity. Modification of AuNPs onto Fe-MOFs can further increase specific surface area to capture large amounts of antibodies and increase electron transfer capacity. With increase of CRP concentration, the oxidation peak current of K3Fe(CN)6/K4Fe(CN)6 decreases, while the oxidation peak current in Fe-MOFs is relatively constant. The response current ratio of K3Fe(CN)6/K4 Fe(CN)6 to Fe-MOFs has been used as a quantitative measurement of CRP response signal. Fe-MOFs modified by K3Fe(CN)6/K4Fe(CN)6 as a signal probe (K3Fe(CN)6/K4Fe(CN)6-sP) and AuNPs as internal reference probes (Fe-MOFs-rP). This ratio probe integrates sP and rP into one structure, ensuring identical modification conditions and the interdependence of sP and rP on a sensing interface. Therefore, this ratiometric probe has a stronger ability to eliminate environmental variation interference. The linear response of the immunosensor proposed here ranges from 1 to 200 ng/mL, and the detection limit is 0.3 ng/mL (S/N=3). The sensor has good selectivity and is expected to be used for early screening and diagnosis of cardiovascular disease. The method also lays the foundation for the preparation of other ratiometric signal amplification sensors for detecting biomarkers.
  • 加载中
    1. [1]

    2. [2]

    3. [3]

    4. [4]

    5. [5]

    6. [6]

    7. [7]

    8. [8]

    9. [9]

    10. [10]

    11. [11]

    12. [12]

    13. [13]

    14. [14]

    15. [15]

    16. [16]

    17. [17]

    18. [18]

    19. [19]

    20. [20]

    21. [21]

    22. [22]

    23. [23]

    24. [24]

    25. [25]

    26. [26]

    27. [27]

    28. [28]

  • 加载中
    1. [1]

      Lin′an CAO , Dengyue MA , Gang XU . Research advances in electrically conductive metal-organic frameworks-based electrochemical sensors. Chinese Journal of Inorganic Chemistry, 2025, 41(10): 1953-1972. doi: 10.11862/CJIC.20250160

    2. [2]

      Tiantian MA , Sumei LI , Chengyu ZHANG , Lu XU , Yiyan BAI , Yunlong FU , Wenjuan JI , Haiying YANG . Methyl-functionalized Cd-based metal-organic framework for highly sensitive electrochemical sensing of dopamine. Chinese Journal of Inorganic Chemistry, 2024, 40(4): 725-735. doi: 10.11862/CJIC.20230351

    3. [3]

      Lu XU , Chengyu ZHANG , Wenjuan JI , Haiying YANG , Yunlong FU . Zinc metal-organic framework with high-density free carboxyl oxygen functionalized pore walls for targeted electrochemical sensing of paracetamol. Chinese Journal of Inorganic Chemistry, 2024, 40(5): 907-918. doi: 10.11862/CJIC.20230431

    4. [4]

      Jing SU , Bingrong LI , Yiyan BAI , Wenjuan JI , Haiying YANG , Zhefeng Fan . Highly sensitive electrochemical dopamine sensor based on a highly stable In-based metal-organic framework with amino-enriched pores. Chinese Journal of Inorganic Chemistry, 2024, 40(7): 1337-1346. doi: 10.11862/CJIC.20230414

    5. [5]

      Yang Meiqing , Lu Wang , Haozi Lu , Yaocheng Yang , Song Liu . Recent Advances of Functional Nanomaterials for Screen-Printed Photoelectrochemical Biosensors. Acta Physico-Chimica Sinica, 2025, 41(2): 100018-0. doi: 10.3866/PKU.WHXB202310046

    6. [6]

      Zhi FANG , Liang SUN , Mingze ZHENG , Wenhao SHENG , Hongliang HUANG , Chongli ZHONG . An aluminum-based metal-organic framework with slit pores for the efficient separation and recovery of electronic specialty gas C3F8. Chinese Journal of Inorganic Chemistry, 2025, 41(10): 2054-2062. doi: 10.11862/CJIC.20250096

    7. [7]

      Jun LUO , Baoshu LIU , Yunchang ZHANG , Bingkai WANG , Beibei GUO , Lan SHE , Tianheng CHEN . Europium(Ⅲ) metal-organic framework as a fluorescent probe for selectively and sensitively sensing Pb2+ in aqueous solution. Chinese Journal of Inorganic Chemistry, 2024, 40(12): 2438-2444. doi: 10.11862/CJIC.20240240

    8. [8]

      Shengbiao Zheng ,  Liang Li ,  Nini Zhang ,  Ruimin Bao ,  Ruizhang Hu ,  Jing Tang . Metal-Organic Framework-Derived Materials Modified Electrode for Electrochemical Sensing of Tert-Butylhydroquinone: A Recommended Comprehensive Chemistry Experiment for Translating Research Results. University Chemistry, 2024, 39(7): 345-353. doi: 10.3866/PKU.DXHX202310096

    9. [9]

      Fugui XI , Du LI , Zhourui YAN , Hui WANG , Junyu XIANG , Zhiyun DONG . Functionalized zirconium metal-organic frameworks for the removal of tetracycline from water. Chinese Journal of Inorganic Chemistry, 2025, 41(4): 683-694. doi: 10.11862/CJIC.20240291

    10. [10]

      Yan Qi ,  Yueqin Yu ,  Weisi Guo ,  Yongjun Liu . 过渡金属参与的有机反应案例教学与实践探索. University Chemistry, 2025, 40(6): 111-117. doi: 10.12461/PKU.DXHX202411021

    11. [11]

      Ruige ZHANG , Zhe ZHANG , He ZHENG , Zhan SHI . Recent advances of metal-organic frameworks for alkaline electrocatalytic oxygen evolution reaction. Chinese Journal of Inorganic Chemistry, 2025, 41(10): 2011-2028. doi: 10.11862/CJIC.20250185

    12. [12]

      Hui-Ying Chen , Hao-Lin Zhu , Pei-Qin Liao , Xiao-Ming Chen . Integration of Ru(Ⅱ)-Bipyridyl and Zinc(Ⅱ)-Porphyrin Moieties in a Metal-Organic Framework for Efficient Overall CO2 Photoreduction. Acta Physico-Chimica Sinica, 2024, 40(4): 2306046-0. doi: 10.3866/PKU.WHXB202306046

    13. [13]

      Mengzhen JIANG , Qian WANG , Junfeng BAI . Research progress on low-cost ligand-based metal-organic frameworks for carbon dioxide capture from industrial flue gas. Chinese Journal of Inorganic Chemistry, 2025, 41(1): 1-13. doi: 10.11862/CJIC.20240355

    14. [14]

      Aiai WANG , Lu ZHAO , Yunfeng BAI , Feng FENG . Research progress of bimetallic organic framework in tumor diagnosis and treatment. Chinese Journal of Inorganic Chemistry, 2024, 40(10): 1825-1839. doi: 10.11862/CJIC.20240225

    15. [15]

      Ran HUO , Zhaohui ZHANG , Xi SU , Long CHEN . Research progress on multivariate two dimensional conjugated metal organic frameworks. Chinese Journal of Inorganic Chemistry, 2024, 40(11): 2063-2074. doi: 10.11862/CJIC.20240195

    16. [16]

      Bin HE , Hao ZHANG , Lin XU , Yanghe LIU , Feifan LANG , Jiandong PANG . Recent progress in multicomponent zirconium?based metal-organic frameworks. Chinese Journal of Inorganic Chemistry, 2024, 40(11): 2041-2062. doi: 10.11862/CJIC.20240161

    17. [17]

      . Synthesis and properties of metal‐organic frameworks. Chinese Journal of Inorganic Chemistry, 2025, 41(10): 1-2.

    18. [18]

      Yongjian Zhang ,  Fangling Gao ,  Hong Yan ,  Keyin Ye . Electrochemical Transformation of Organosulfur Compounds. University Chemistry, 2025, 40(5): 311-317. doi: 10.12461/PKU.DXHX202407035

    19. [19]

      Xinwan Zhao , Yue Cao , Minjun Lei , Zhiliang Jin , Tsubaki Noritatsu . Constructing S-scheme heterojunctions by integrating covalent organic frameworks with transition metal sulfides for efficient noble-metal-free photocatalytic hydrogen evolution. Acta Physico-Chimica Sinica, 2025, 41(12): 100152-0. doi: 10.1016/j.actphy.2025.100152

    20. [20]

      Jiarong Feng ,  Yejie Duan ,  Chu Chu ,  Dezhen Xie ,  Qiu'e Cao ,  Peng Liu . Preparation and Application of a Streptomycin Molecularly Imprinted Electrochemical Sensor: A Suggested Comprehensive Analytical Chemical Experiment. University Chemistry, 2024, 39(8): 295-305. doi: 10.3866/PKU.DXHX202401016

Get Citation
PDF
XML
Metrics
  • PDF Downloads(11)
  • Abstract views(829)
  • HTML views(68)

通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索
Address：Zhongguancun North First Street 2,100190 Beijing, PR China Tel: +86-010-82449177-888
Powered By info@rhhz.net

/

DownLoad:  Full-Size Img  PowerPoint
Return