裂解气相色谱-质谱法分析土壤有机质的化学组成

引用本文: 蒋逸凡, 喻文娟, 王姣娇, 康宏樟. 裂解气相色谱-质谱法分析土壤有机质的化学组成[J]. 分析化学, 2020, 48(11): 1526-1534. doi: 10.19756/j.issn.0253-3820.191630 shu

Citation: JIANG Yi-Fan, YU Wen-Juan, WANG Jiao-Jiao, KANG Hong-Zhang. Analysis of Chemical Composition of Soil Organic Matter Using Pyrolysis Gas Chromatography Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, 2020, 48(11): 1526-1534. doi: 10.19756/j.issn.0253-3820.191630 shu

裂解气相色谱-质谱法分析土壤有机质的化学组成

蒋逸凡 ^1, ,
喻文娟 ^{2,
,} ,
王姣娇 ^1,3, ,
康宏樟 ^{1,4,
,}

1.
上海交通大学农业与生物学院, 上海 200240;
2.
Department of Ecology, Evolution, and Organismal Biology, Iowa State University, Ames, IA 50011 美国;
3.
上海长三角人口密集区生态环境变化与综合治理教育部野外科学观测研究站, 上海 200240;
4.
国家林业和草原局上海城市森林生态系统国家定位观测研究站, 上海 200240

通讯作者: 喻文娟,wjyu@iastate.edu; 康宏樟,kanghz@sjtu.edu.cn

基金项目:
本文系国家重点研发计划项目（No.2017YFC0505501）和国家自然科学基金项目（Nos.31770746，31270491）资助

摘要: 土壤有机质（SOM）是土壤的重要组成部分，对土壤的肥力、水分含量等各种性质具有重要作用。本研究采集了中国东部7个地区不同深度的土壤，采用直接进样-热裂解-气相色谱/质谱法（Py-GC/MS，直接进样法）和四甲基氢氧化铵衍生-热裂解-气相色谱/质谱法（TMAH-Py-GC/MS，TMAH衍生法），对土壤有机质进行了定性和半定量分析。TMAH衍生法采用25% TMAH的甲醇溶液浸没24 h进行衍生化，选择DB-5 ms（30 m×0.25 mm，0.25 μm）色谱柱进行气相色谱-质谱分析，测试条件与直接进样法相同。TMAH衍生法和直接进样法分别鉴定出261种和117种土壤有机质的标识物，TMAH衍生法检测到的物质种类更多、结构更多样，显著提高了木质素类和脂质类标识物的数目。两种方法检测出的各类物质含量也存在差异，直接进样法中多糖（28%）、脂质（22%）占比较高；TMAH衍生法中脂质（33%）含量较高，其它类物质除酚类化合物外，含量均接近10%。烷烃、烯烃、脂肪酸等物质的色谱峰保留时间与碳原子数之间线性关系显著，相关系数（R²）均大于0.95。同时还对比了两种方法所获得的六大类物质百分含量随土壤深度与纬度变化规律的异同。

关键词:

English

Analysis of Chemical Composition of Soil Organic Matter Using Pyrolysis Gas Chromatography Mass Spectrometry

1.
School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China;
2.
Department of Ecology, Evolution, and Organismal Biology, Iowa State University, Ames, IA 50011, USA;
3.
Yangtze River Delta Ecology&Environmental Change and Control Research Station, Ministry of Education, Shanghai 200240, China;
4.
Shanghai Urban Forest Ecosystem Research Station, National Forestry and Grassland Administration, Shanghai 200240, China

Corresponding author: YU Wen-Juan, ; KANG Hong-Zhang,

Received Date: 24 October 2019
Revised Date: 28 August 2020
Fund Project:
This work was supported by the National Key R&D Program of China (No. 2017YFC0505501) and the National Natural Science Foundation of China (Nos. 31770746, 31270491).

Abstract: Soil organic matter (SOM) is an important part of soil, which plays an important role in soil fertility, water content and other properties. In this work, soil samples were collected from seven oriental oak sites at different depths along a latitudinal gradient in eastern China. The chemical composition of SOM of 84 samples was analyzed qualitatively and semi-quantitatively by direct injection pyrolysis gas chromatography/mass spectrometry (Py-GC/MS) and tetramethylammonium hydroxide derivatization pyrolysis gas chromatography/mass spectrometry (TMAH-Py-GC/MS), respectively. TMAH methanol solution (25%) was used for derivatization, and a DB-5ms (30 m×0.25 mm, 0.25 μm) column was selected for GC/MS analysis. A total of 261 and 117 biomarkers of SOM were identified by TMAH-Py-GC/MS and Py-GC/MS methods, respectively. The markers detected by the TMAH-Py-GC/MS method had more types and more diverse structures and included more numbers of lignin-derived and lipid-derived compounds. The contents of substances also varied with methods. The Py-GC/MS samples had higher proportions of polysaccharides (28%) and lipids (22%), while the TMAH-Py-GC/MS samples had higher contents of lipids (33%). The chromatographic retention times of alkanes, alkenes, and fatty acids all had good linear relationship with numbers of carbon atom (R²>0.95). The changes of contents of six groups with soil depth and latitude obtained by the two methods were compared.

Key words:

Soil organic matter
/ Tetramethylammonium hydroxide
/ Chemical composition
/ Pyrolysis gas chromatography-mass spectrometry

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沈阳化工大学材料科学与工程学院沈阳 110142

裂解气相色谱-质谱法分析土壤有机质的化学组成

通讯作者: 喻文娟,wjyu@iastate.edu; 康宏樟,kanghz@sjtu.edu.cn

English

Analysis of Chemical Composition of Soil Organic Matter Using Pyrolysis Gas Chromatography Mass Spectrometry

Corresponding author: YU Wen-Juan, ; KANG Hong-Zhang,

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

裂解气相色谱-质谱法分析土壤有机质的化学组成

通讯作者: 喻文娟,wjyu@iastate.edu; 康宏樟,kanghz@sjtu.edu.cn

English

Analysis of Chemical Composition of Soil Organic Matter Using Pyrolysis Gas Chromatography Mass Spectrometry

Corresponding author: YU Wen-Juan, ; KANG Hong-Zhang,

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

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CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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