基于超高效液相色谱-高分辨质谱联用技术研究冠心病及冠心病合并2型糖尿病患者代谢特征

引用本文: 任繁栋, 丁筱雪, 蔡芳, 任达兵, 易伦朝, 张宏. 基于超高效液相色谱-高分辨质谱联用技术研究冠心病及冠心病合并2型糖尿病患者代谢特征[J]. 分析化学, 2020, 48(1): 49-56. doi: 10.19756/j.issn.0253-3820.191618 shu

Citation: REN Fan-Dong, DING Xiao-Xue, CAI Fang, REN Da-Bing, YI Lun-Zhao, ZHANG Hong. Investigation of Metabolic Features of Patients with Coronary Heart Disease or Coronary Heart Disease-Type 2 Diabetes Mellitus Based on Ultra-High Performance Liquid Chromatography-High Resolution Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, 2020, 48(1): 49-56. doi: 10.19756/j.issn.0253-3820.191618 shu

基于超高效液相色谱-高分辨质谱联用技术研究冠心病及冠心病合并2型糖尿病患者代谢特征

任繁栋 ^1, ,
丁筱雪 ^2, ,
蔡芳 ^1, ,
任达兵 ^1, ,
易伦朝 ^1, ,
张宏 ^2,

1.
昆明理工大学农业与食品学院, 昆明 650504;
2.
云南省第一人民医院心血管内科, 昆明 650032
基金项目:
本文系国家自然科学基金项目（No.21775058）和云南省应用基础研究项目（昆医联合专项）（No.2017FE468（-169））资助

摘要: 采用超高效液相色谱-四极杆-轨道阱质谱联用技术开展冠心病及冠心病合并2型糖尿病患者的血浆代谢组学研究，以探寻冠心病及冠心病合并2型糖尿病患者的代谢特征，为基于代谢组学的临床治疗方案优化奠定基础。本研究鉴定出78种血浆中的内源性代谢物。在代谢物定性与定量分析基础上，结合偏最小二乘-判别分析和变量重要性投影等方法，筛选出20种区分健康人和冠心病患者的潜在特征代谢物、35种区分冠心病及冠心病合并2型糖尿病患者的潜在特征代谢物、37种区分健康人和冠心病合并2型糖尿病的潜在特征代谢物。代谢通路分析结果表明，氨基酸代谢在这两类患者的生理代谢过程中发挥重要作用，而脂肪酸代谢很可能与冠心病合并2型糖尿病相关。

关键词:

English

Investigation of Metabolic Features of Patients with Coronary Heart Disease or Coronary Heart Disease-Type 2 Diabetes Mellitus Based on Ultra-High Performance Liquid Chromatography-High Resolution Mass Spectrometry

1.
College of Agriculture and Food Science, Kunming University of Science and Technology, Kunming 650504, China;
2.
Department of Cardiology, Yunnan First People's Hospital, Kunming 650032, China
Received Date: 21 October 2019
Revised Date: 26 November 2019
Fund Project:
This work was supported by the National Natural Science Foundation of China (No. 21775058) and the Yunnan Provincial Applied Basic Research Project (Kun Medical Joint Project) (No. 2017FE468 (-169)).

Abstract: Ultra-high performance liquid chromatography-quadrupole-orbitrap mass spectrometry was used in plasma metabolomics study of patients with coronary heart disease and coronary heart disease-type 2 diabetes mellitus. The aim was to explore the metabolic characteristics of coronary heart disease and coronary heart disease-type 2 diabetes mellitus, which would be useful for the optimization of clinical treatment plans based on metabolomics. A total of 78 endogenous metabolites were analyzed in plasma. On this basis, combined with partial least squares-discriminant analysis and variable importance projection, 20 characteristic metabolites were screened to distinguish healthy control from patients with coronary heart disease, 35 metabolites were screened for discrimination of patients with coronary heart disease and coronary heart disease-type 2 diabetes mellitus, 37 metabolites were screened to distinguish healthy control from patients with coronary heart disease-type 2 diabetes mellitus. Metabolic pathway analysis showed that amino acid metabolism played an important role in the physiological metabolism of these two types of patients, and fatty acid metabolism was likely to be associated with the complication of type 2 diabetes mellitus.

Key words:

Coronary heart disease
/ Coronary heart disease-type 2 diabetes mellitus
/ Ultra-high performance liquid chromatography-high resolution mass spectrometry
/ Metabolomics
/ Chemometrics

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基于超高效液相色谱-高分辨质谱联用技术研究冠心病及冠心病合并2型糖尿病患者代谢特征

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Investigation of Metabolic Features of Patients with Coronary Heart Disease or Coronary Heart Disease-Type 2 Diabetes Mellitus Based on Ultra-High Performance Liquid Chromatography-High Resolution Mass Spectrometry

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