基于超高效液相色谱-高分辨质谱联用技术研究冠心病及冠心病合并2型糖尿病患者代谢特征

任繁栋 丁筱雪 蔡芳 任达兵 易伦朝 张宏

引用本文: 任繁栋,  丁筱雪,  蔡芳,  任达兵,  易伦朝,  张宏. 基于超高效液相色谱-高分辨质谱联用技术研究冠心病及冠心病合并2型糖尿病患者代谢特征[J]. 分析化学, 2020, 48(1): 49-56. doi: 10.19756/j.issn.0253-3820.191618 shu
Citation:  REN Fan-Dong,  DING Xiao-Xue,  CAI Fang,  REN Da-Bing,  YI Lun-Zhao,  ZHANG Hong. Investigation of Metabolic Features of Patients with Coronary Heart Disease or Coronary Heart Disease-Type 2 Diabetes Mellitus Based on Ultra-High Performance Liquid Chromatography-High Resolution Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, 2020, 48(1): 49-56. doi: 10.19756/j.issn.0253-3820.191618 shu

基于超高效液相色谱-高分辨质谱联用技术研究冠心病及冠心病合并2型糖尿病患者代谢特征

  • 基金项目:

    本文系国家自然科学基金项目(No.21775058)和云南省应用基础研究项目(昆医联合专项)(No.2017FE468(-169))资助

摘要: 采用超高效液相色谱-四极杆-轨道阱质谱联用技术开展冠心病及冠心病合并2型糖尿病患者的血浆代谢组学研究,以探寻冠心病及冠心病合并2型糖尿病患者的代谢特征,为基于代谢组学的临床治疗方案优化奠定基础。本研究鉴定出78种血浆中的内源性代谢物。在代谢物定性与定量分析基础上,结合偏最小二乘-判别分析和变量重要性投影等方法,筛选出20种区分健康人和冠心病患者的潜在特征代谢物、35种区分冠心病及冠心病合并2型糖尿病患者的潜在特征代谢物、37种区分健康人和冠心病合并2型糖尿病的潜在特征代谢物。代谢通路分析结果表明,氨基酸代谢在这两类患者的生理代谢过程中发挥重要作用,而脂肪酸代谢很可能与冠心病合并2型糖尿病相关。

English


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  • 收稿日期:  2019-10-21
  • 修回日期:  2019-11-26
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