蛋白质介导硫化铜键合的介孔硅纳米载体的构筑及其用于癌症的光热和化疗协同治疗的研究

引用本文: 何若曦, 王琦, 李贝, 贾晶, 路雯婧, 双少敏. 蛋白质介导硫化铜键合的介孔硅纳米载体的构筑及其用于癌症的光热和化疗协同治疗的研究[J]. 分析化学, 2020, 48(2): 197-205. doi: 10.19756/j.issn.0253-3820.191615 shu

Citation: HE Ruo-Xi, WANG Qi, LI Bei, JIA Jing, LU Wen-Jing, SHUANG Shao-Min. Construction of Protein-mediated Copper Sulfide Bonded Mesoporous Silica Nanoparticles Vector for Chemo-photothermal Synergistic Therapy of Cancer[J]. Chinese Journal of Analytical Chemistry, 2020, 48(2): 197-205. doi: 10.19756/j.issn.0253-3820.191615 shu

蛋白质介导硫化铜键合的介孔硅纳米载体的构筑及其用于癌症的光热和化疗协同治疗的研究

山西大学化学化工学院, 太原 030006
基金项目:
本文系国家自然科学基金项目（No.21874087）资助

摘要: 将牛血清白蛋白介导的硫化铜纳米复合物（BSA/CuS）通过酰胺键修饰在负载了化疗药物阿霉素（DOX）的介孔二氧化硅（MSN）表面，制备了新型药物载体MSN-DOX@BSA/CuS。此复合物中BSA作为封闭剂，其二硫键与谷胱甘肽发生氧化还原反应后断裂，导致MSN孔隙暴露，使DOX从中释放；CuS作为光热试剂，可将光能转化为热能，具有光热治疗的作用。利用紫外-可见-近红外吸收光谱考察了其光吸收性质。结果表明，此药物载体在近红外区800~1100 nm范围内具有较强的吸收，可在近红外光照射下实现光热转换。另外，将未负载DOX的载体MSN@BSA/CuS与癌细胞孵育，此载体表现出低细胞毒性；加入负载药物的MSN-DOX@BSA/CuS后，细胞的存活率降为49%；经过额外的激光照射后，细胞存活率仅为18%。以上结果表明，所合成的纳米药物载体可同时实现光热治疗与化疗的协同治疗，有效提高对癌症的治疗效率。

关键词:

English

Construction of Protein-mediated Copper Sulfide Bonded Mesoporous Silica Nanoparticles Vector for Chemo-photothermal Synergistic Therapy of Cancer

School of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, China
Received Date: 19 October 2019
Revised Date: 21 November 2019
Fund Project:
This work was supported by the National Natural Science Foundation of China (No. 21874087).

Abstract: Bovine serum albumin-mediated copper sulfide nanocomplex (BSA/CuS) was modified on mesoporous silica (MSN) loaded with chemotherapeutic drug doxorubicin (DOX) by amide bond to prepare a new drug carrier, MSN-DOX@BSA/CuS. The disulfide bond of blocking agent BSA would break down after redox reaction with glutathione, resulting in MSN pore exposure and release of DOX. CuS, as a photothermal reagent, could convert light energy into heat for photothermal therapy. UV-Vis-NIR absorption spectra were used to investigate the absorption properties of the carrier. The results showed that the carrier had strong absorption in the range of 800-1100 nm and could realize the photothermal conversion under the near infrared light. In addition, MSN@BSA/CuS was incubated with cancer cells and showed no cytotoxicity. When MSN-DOX@BSA/CuS was added, the relative survival rate of the cells decreased to 49%. After the extra laser, the cell viability was only 18%. The above results showed that the synthesized nano drug carrier could realize the synergistic treatment of photothermal therapy and chemotherapy at the same time, and effectively improved the treatment efficiency of cancer.

Key words:

1. [1]
  Feng L L, Xie R, Wang C Q, Gai S L, He F, Yang D, Yang P P, Lin J. ACS Nano,2018,12(11):11000-11012
2. [2]
  Chen C, Tang W, Jiang D W, Yang G L, Wang X L, Zhou L N, Zhang W A, Wang P. Nanoscale,2019,11(22):11012-11024
3. [3]
  Li J, Zhang C T, Gong S M, Li X C, Yu M Y, Qian C G, Qiao H Z, Sun M J. Acta Biomater.,2019,94:435-446
4. [4]
  Girma W M, Dehvari K, Ling Y C, Chang J Y. Mater. Sci. Eng. C,2019,101:179-189
5. [5]
  Cai C, Li X, Wang Y, Liu M X, Shi X Y, Xia J D, Shen M W. Chem. Eng. J.,2019,362:842-850
6. [6]
  Rahoui N, Jiang B, Hegazy M, Taloub N, Wang Y L, Yu M, Huang Y D. Colloids Surf. B,2018,171:176-185
7. [7]
  Su T, Cheng F R, Yan J Q, Cao J, Luo K, Pu Y J, He B. J. Mater. Chem. B,2018,6(28):4687-4696
8. [8]
  Zheng Y Y, Liang Y Q, Zhang D P, Zhou Z J, Li J, Sun X Y, Liu Y N. Chem. Commun.,2018,54(98):13805-13808
9. [9]
  Liu X M, Yang T S, Han Y F, Zou L, Yang H R, Jiang J Y, Liu S J, Zhao Q, Huang W. ACS Appl. Mater. Interfaces,2018,10(37):31008-31018
10. [10]
  Yang W T, Guo W S, Le W J, Lv G X, Zhang F H, Shi L, Wang X L, Wang J, Wang S, Chang J, Zhang B B. ACS Nano,2016,10(11):10245-10257
11. [11]
  Yang Y, Wang C, Tian C, Guo H L, Shen Y H, Zhu M Z. J. Mater. Chem. B,2018,6(42):6848-6857
12. [12]
  Goel S, Ferreira C A, Chen F, Ellison P A, Siamof C M, Barnhart T E, Cai W B. Adv. Mater.,2018,30(6):1704367
13. [13]
  Liu P, Wang Y R, An L, Tian Q W, Lin J M, Yang S P. ACS Appl. Mater. Interfaces,2018,10(45):38833-38844
14. [14]
  Wang Y Y, Liu X J, Deng G Y, Sun J, Yuan H K, Li Q, Wang Q G, Lu J. Nanoscale,2018,10(6):2866-2875
15. [15]
  Peng S W, He Y Y, Er M, Sheng Y Z, Gu Y Q, Chen H Y. Biomater. Sci.,2017,5(3):475-484
16. [16]
  Fan W P, Yung B, Huang P, Chen X Y. Chem. Rev.,2017,117(22):13566-13638
17. [17]
  Zhang L, Li Y C, Jin Z X, Chan K M, Yu J C. RSC Adv., 2015,5(113):93226-93233
18. [18]
  Lin X D, Fang Y, Tao Z H, Gao X, Wang T L, Zhao M Y, Wang S, Liu Y Q. ACS Appl. Mater. Interfaces,2019,11(28):25043-25053
19. [19]
  Xu Z Q, Wu S C, Huang G C, Ding H W, Gui R J, Zhu B H. Mater. Lett.,2017,195:131-135
20. [20]
  Liu X J, Ren Q L, Fu F F, Zou R J, Wang Q, Xin G B, Xiao Z Y, Huang X J, Liu Q, Hu J Q. Dalton Trans.,2015,44(22):10343-10351
21. [21]
  Cheng W, Nie J P, Xu L, Liang C Y, Peng Y M, Liu G, Wang T, Mei L, Huang L Q, Zeng X W. ACS Appl. Mater. Interfaces,2017,9(22):18462-18473
22. [22]
  Shen B B, Ma Y, Yu S Y, Ji C H. ACS Appl. Mater. Interfaces,2016,8(37):24502-24508
23. [23]
  Lin L S, Song J B, Song L, Ke K M, Liu Y J, Zhou Z J, Shen Z Y, Li J, Yang Z, Tang W, Niu G, Yang H H, Chen X Y. Angew. Chem. Int. Ed.,2018,57(18):4902-4906
24. [LM]24 Gao Y, Zhong S L, Xu L F, He S H, Dou Y M, Zhao S N, Chen P, Cui X J. Micropor. Mesopor Mater.,2019,278:130-137
25. [25]
  Zhang Y B, Li Y, Tian H N, Zhu Q X, Wang F F, Fan Z X, Zhou S, Wang X W, Xie L Y, Hou Z Q. Mol. Pharmaceut.,2019,16(7):3133-3144
26. [26]
  Song Y H, Li Y H, Xu Q E, Liu Z. Int. J. Nanomed.,2017,12:87-110
27. [27]
  Paris J L, Cabañas M V, Manzano M, Vallet-Regí M. ACS Nano,2015,9(11):11023-11033

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沈阳化工大学材料科学与工程学院沈阳 110142

蛋白质介导硫化铜键合的介孔硅纳米载体的构筑及其用于癌症的光热和化疗协同治疗的研究

English

Construction of Protein-mediated Copper Sulfide Bonded Mesoporous Silica Nanoparticles Vector for Chemo-photothermal Synergistic Therapy of Cancer

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

蛋白质介导硫化铜键合的介孔硅纳米载体的构筑及其用于癌症的光热和化疗协同治疗的研究

English

Construction of Protein-mediated Copper Sulfide Bonded Mesoporous Silica Nanoparticles Vector for Chemo-photothermal Synergistic Therapy of Cancer

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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通讯作者: 陈斌, bchen63@163.com

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