基于仿生纳米导线的电化学传感器对水体重金属毒性的检测分析

引用本文: 王羽, 王蔚港, 杨向黎, 尹伶灵, 商冉, 李霞, 陈燕. 基于仿生纳米导线的电化学传感器对水体重金属毒性的检测分析[J]. 分析化学, 2021, 49(1): 68-75. doi: 10.19756/j.issn.0253-3820.191611 shu

Citation: WANG Yu, WANG Wei-Gang, YANG Xiang-Li, YING Ling-Ling, SHANG Ran, LI Xia, CHEN Yan. Detection of Acute Toxicity of Water Using Electrochemical Sensor Based on Bionic Nanowire[J]. Chinese Journal of Analytical Chemistry, 2021, 49(1): 68-75. doi: 10.19756/j.issn.0253-3820.191611 shu

基于仿生纳米导线的电化学传感器对水体重金属毒性的检测分析

王羽 ^1, ,
王蔚港 ^1, ,
杨向黎 ^1, ,
尹伶灵 ^2, ,
商冉 ^1, ,
李霞 ^1, ,
陈燕 ^{1,
,}

1.
山东农业工程学院资源与环境工程学院, 济南 250100;
2.
山东省兽药质量检验所山东省畜产品质量安全监测与风险评估重点实验室, 济南 250022

通讯作者: 陈燕,E-mail:ychen0612@163.com

基金项目:
山东省高等学校科技计划项目（No.J16LE56）、全国大学生创新创业项目（No.201714439007）、国家自然科学基金项目（No.61801274）和山东省高等学校青年创新团队发展计划项目（No.2019KJF029）资助。

摘要: 准确快速诊断水体重金属类污染物的毒性对保障饮用水安全至关重要。本研究基于大肠杆菌（E.coli）细胞膜受损的毒性响应机理，以K₃Fe（CN）₆作为胞外电子受体，碳纳米管（CNTs）作为仿生纳米导线，建立了一种基于电化学传感器的水体重金属毒性检测新方法。考察了电极修饰材料、菌体浓度、电子传递体与毒性物质加入顺序、K₃Fe（CN）₆浓度、菌体与毒物的接触时间等因素对传感器灵敏度的影响。在最佳实验条件下，测得单一金属离子Zn²⁺、Cd²⁺、Cu²⁺、Ag⁺和Pb²⁺对E.coli毒性的半数抑制浓度（IC₅₀）分别为10.72、15.03、6.14、16.28和20.98 mg/L。本方法操作简单、检测成本低、检测时间短且结果准确、可靠，有望用于水体急性毒性的快速预警和筛查，具有重要的实际应用价值。

关键词:

English

Detection of Acute Toxicity of Water Using Electrochemical Sensor Based on Bionic Nanowire

1.
School of Resources and Environmental Engineering, Shandong Agriculture and Engineering University, Jinan 250100, China;
2.
Shandong Provincial Veterinary Medicine Supervision Institute/Shandong Provincial Key Laboratory of Quality Safety Monitoring and Risk Assessment for Livestock and Poultry Products, Jinan 250022, China

Corresponding author: CHEN Yan, ychen0612@163.com

Received Date: 16 October 2019
Revised Date: 06 August 2020
Fund Project:
Supported by the Scientific Research Foundation of the Higher Education Institutions of Shandong Province, China (No. J16LE56), the National Undergraduate Training Programs for Innovation and Entrepreneurship (No. 201714439007), the National Natural Science Foundation of China (No.61801274), and the Qingchuang Science and Technology Program of Shandong Province in China (No.2019KJF029).

Abstract: It is important to rapidly diagnose heavy metal toxicity of water for drinking water safety. Based on Escherichia coli (E.coli) cell damage, in this work, a new toxicity bioassay using multi-walled carbon nanotubes (MWCNTs) as bionic nanowire and K₃Fe(CN)₆ as electron transporter was developed. The influences of modified materials, optical density of E.coli, incubation time and K₃Fe(CN)₆ concentration on the respiratory inhibition of E.coil were investigated. Under the best conditions, IC₅₀ values of single metal ions (Zn²⁺, Cd²⁺, Cu²⁺, Ag⁺ and Pb²⁺) were 10.72, 15.03, 6.14, 16.28 and 20.98 mg/L, respectively. This method had virtues of simple operation, low cost, short detection time, accurate and reliable results, and might serve as an early warning device for rapid warning and screening of water acute toxicity, thus avoiding environmental pollution and protecting public health.

Key words:

1. [1]
  ARAGAY G, PONS J, MERKOCI A. Chem. Rev., 2011, 111(5):3433-3458.
2. [2]
  KIM H N, REN W X, KIM J S, YOON J. Chem. Soc. Rev., 2012, 41(8):3210-3244.
3. [3]
  LI J, GUO S J, ZHAI Y M, WANG E K. Anal. Chim. Acta, 2009, 649(2):196-201.
4. [4]
  ISAACSON C W, USENKO C Y, TANGUAY R L, FIELD J A. Anal. Chem., 2007, 79(23):9091-9097.
5. [5]
  DIESEL E, SCHREIBER M, VAN DER MEER J R. Anal. Bioanal. Chem., 2009, 394(3):687-693.
6. [6]
  PUJOL-VILA F, VIGUÉS N, DÍAZ-GONZÁLEZ M, MUÑOZ-BERBEL X, MAS J. Biosens. Bioelectron., 2015, 57:272-279.
7. [7]
  FANG D Y, YU Y, WU L Z, WANG Y, ZHANG J H, ZHI J F. RSC Adv., 2015, 73(5):59472-59479.
8. [8]
  ZHAI J F, YONG D M, LI J, DONG S J. Analyst, 2013, 138:702-707.
9. [9]
  YU D B, ZHAI J F, YONG D M, DONG S J. Analyst, 2013, 138:3297-3302.
10. [10]
  MA H P, YONG D M, KIM H, ZHANG Z J, MA S H, HAN X J. Electroanalysis, 2016, 28:580-587.
11. [11]
  ZHAI Jun-Feng, YU Deng-Bin, LIU Ling, DONG Shao-Jun. Chin. J. Appl. Chem., 2018, 35(9):1102-1106. 翟俊峰, 余登斌, 刘玲, 董绍俊. 应用化学, 2018, 35(9):1102-1106.
12. [12]
  MORRIS K, ZHAO H J, JOHN R. Aust. J. Chem., 2005, 58:237-245.
13. [13]
  CATTERALL K, ROBERTSON D, HUDSON S, TEASDALE P R, WELSH D T, John R. Talanta, 2010, 82:751-757.
14. [14]
  YU Deng-Bin, JIANG Lan, WANG Pei, LI Ting, DONG Shao-Jun. Chin. J. Anal. Chem., 2018, 46(7):1032-1038. 余登斌, 江岚, 王沛, 李婷, 董绍俊. 分析化学, 2018, 46(7):1032-1038.
15. [15]
  ZHAI Jun-Feng, YU Deng-Bin, DONG Shao-Jun. Chin. J. Anal. Chem., 2017, 45(9):1415-1419. 翟俊峰, 余登斌, 董绍俊. 分析化学, 2017, 45(9):1415-1419.
16. [16]
  YONG D M, LIU C Y, ZHU C Z, YU D B, LIU L, ZHAI J F, DONG S J. Chemosphere, 2015, 139:109-116.
17. [17]
  ZHAI Jun-Feng, LIU Ling, YU Deng-Bin, DONG Shao-Jun. China Patent, 201610344913.4, 2019. 翟俊峰, 刘玲, 余登斌, 董绍俊. 中国专利, 201610344913.4, 2019.
18. [18]
  DONG Shao-Jun, YONG Da-Ming. China Patent, 201210004805.4, 2014. 董绍俊, 雍达明. 中国专利, 201210004805.4, 2014.
19. [19]
  YU D B, ZHAI J F, LIU C Y, ZHANG X P, BAI L, WANG Y Z, DONG S J. ACS Sens., 2017, 2(11):1637-1643.
20. [20]
  FANG D Y, GAO G Y, SHEN J, YU Y, ZHI J F. Electrochim. Acta, 2016, 222:303-311.
21. [21]
  QIAN Jun, LI Jiu-Ming, ZHI Jin-Fang, QIN Shi-Dong. Chin. J. Anal. Chem. 2013, 41(5):738-743. 钱俊, 李久铭, 只金芳, 覃事栋. 分析化学, 2013, 41(5):738-743.
22. [22]
  YANG Y J, FANG D Y, LIU Y R, LIU R Z, WANG X S,YU Y, ZHI J F. Biosens. Bioelectron., 2018, 108:82-88.
23. [23]
  GAO G Y, FANG D Y, YU Y, WU L Z, WANG Y, ZHI J F. Talanta, 2017, 167:208-216.
24. [24]
  YANG Y, FANG Z, YU Y Y, WANG Y Z, NARAGINTI S, YONG Y C. Water Sci. Technol., 2019, 79(6):1071-1080.
25. [25]
  SUN Yan-Yi, WU Kang-Bing, HU Sheng-Shui. Chem. J. Chin. Univ., 2002, 23(11):2067-2069. 孙延一, 吴康兵, 胡胜水. 高等学校化学学报, 2002, 23(11):2067-2069.
26. [26]
  WANG J, MUSAMEH M, LIN Y H. J. Am. Chem. Soc., 2003, 125(9):2408-2409.
27. [27]
  GREENE J C, MILLER W E, DEBACON M K, LONG M A, BARTELS C L. Arch. Environ. Contam. Toxicol., 1985, 14:659-667.
28. [28]
  HSIEH C Y, TSAI M H, RYAN D K, PANCORBO O C. Sci. Total Environ., 2004, 320:37-50.

扫一扫看文章

计量

PDF下载量: 0
文章访问数: 111
HTML全文浏览量: 3

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

基于仿生纳米导线的电化学传感器对水体重金属毒性的检测分析

通讯作者: 陈燕,E-mail:ychen0612@163.com

English

Detection of Acute Toxicity of Water Using Electrochemical Sensor Based on Bionic Nanowire

Corresponding author: CHEN Yan, ychen0612@163.com

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南：你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

计量

通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

基于仿生纳米导线的电化学传感器对水体重金属毒性的检测分析

通讯作者: 陈燕,E-mail:ychen0612@163.com

English

Detection of Acute Toxicity of Water Using Electrochemical Sensor Based on Bionic Nanowire

Corresponding author: CHEN Yan, ychen0612@163.com

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

计量

文章相关

通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

CCS Chemistry：颜徐州、鲍哲南：你的皮肤可能是一片SPMs