高效液相色谱/三重四极杆质谱联用法测定血浆和尿液中鹅膏肽类毒素

魏佳会 陈佳 吴弼东 陈作红 吴剑峰 谢剑炜

引用本文: 魏佳会,  陈佳,  吴弼东,  陈作红,  吴剑峰,  谢剑炜. 高效液相色谱/三重四极杆质谱联用法测定血浆和尿液中鹅膏肽类毒素[J]. 分析化学, 2020, 48(3): 405-412. doi: 10.19756/j.issn.0253-3820.191604 shu
Citation:  WEI Jia-Hui,  CHEN Jia,  WU Bi-Dong,  CHEN Zuo-Hong,  WU Jian-Feng,  XIE Jian-Wei. Determination of Amanita Peptides in Human Plasma and Urine by High-Performance Liquid Chromatography Coupled with Triple Quadrupole Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, 2020, 48(3): 405-412. doi: 10.19756/j.issn.0253-3820.191604 shu

高效液相色谱/三重四极杆质谱联用法测定血浆和尿液中鹅膏肽类毒素

摘要: 建立了高效液相色谱/三重四极杆质谱联用法测定人体血浆及尿液样本中3类5种鹅膏肽类毒素的分析方法。样品经固相萃取分离富集后,在CAPCELL CORE C18色谱柱上分离,流动相为10 mmol/L乙酸铵-甲醇,采用梯度洗脱分离,正离子多反应监测模式(MRM)检测。血浆和尿液样品中5种鹅膏肽类毒素在较宽浓度范围内均呈良好线性关系,检出限为0.2和0.5 μg/L (S/N=3);加标回收率分别为98.0%~114.6%和90.1%~99.4%,准确度、精密度及基质效应等均符合方法学要求。本方法操作简便、分析速度快、灵敏度高,并被应用于鹅膏菌中毒实际样本中鹅膏肽类毒素的检测,为中毒病人的及时救治提供了准确、可靠的技术支持。

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  • 收稿日期:  2019-10-14
  • 修回日期:  2019-12-31
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