纳米铁氰化钴电化学传感器检测细胞释放的超氧阴离子

崔敏 温晓芳 任聚杰 张聪 李娜 韩元元 余旭东 籍雪平

引用本文: 崔敏,  温晓芳,  任聚杰,  张聪,  李娜,  韩元元,  余旭东,  籍雪平. 纳米铁氰化钴电化学传感器检测细胞释放的超氧阴离子[J]. 分析化学, 2020, 48(2): 224-232. doi: 10.19756/j.issn.0253-3820.191600 shu
Citation:  CUI Min,  WEN Xiao-Fang,  REN Ju-Jie,  ZHANG Cong,  LI Na,  HAN Yuan-Yuan,  YU Xu-Dong,  JI Xue-Ping. Electrochemical Detection of Superoxide Anion Released from Living Cells Using Nano Cobalt Ferricyanide[J]. Chinese Journal of Analytical Chemistry, 2020, 48(2): 224-232. doi: 10.19756/j.issn.0253-3820.191600 shu

纳米铁氰化钴电化学传感器检测细胞释放的超氧阴离子

  • 基金项目:

    本文系国家自然科学基金项目(No.81872669)与河北省高等学校科学技术研究项目(Nos.ZD2018037,QN2019140)资助

摘要: 采用共沉淀方法制备的铁氰化钴(Co3[Fe(CN)6]2)和Hummers法制备的还原氧化石墨烯(RGO)共沉积修饰玻碳电极(GCE),制备了无酶型超氧阴离子(O2·-)电化学传感器(Co3[Fe(CN)6]2-RGO/GCE)。采用扫描电子显微镜(SEM)、X射线衍射(XRD)、电化学交流阻抗法(EIS)、循环伏安法(CV)等研究了复合材料Co3[Fe(CN)6]2-RGO的特征及其对水中O2·-的电化学检测的可行性,分析了Co3[Fe(CN)6]2-RGO/GCE检测O2·-的作用机理。传感器对O2·-浓度的检测范围为0.5~30 μmol/L,灵敏度为91.8 μA L/(μmol cm2),检出限为0.071 μmol/L(S/N=3),而且具有良好的选择性,H2O2、O2及其它体内电化学活性物质均不干扰O2·-的测定,成功地应用于MCF-7细胞中释放的O2·-的电化学检测。此传感器在病理和生物学领域定量测定O2·-方面具有潜在的应用价值。

English


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  • 收稿日期:  2019-10-12
  • 修回日期:  2019-12-06
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