纳米铁氰化钴电化学传感器检测细胞释放的超氧阴离子

引用本文: 崔敏, 温晓芳, 任聚杰, 张聪, 李娜, 韩元元, 余旭东, 籍雪平. 纳米铁氰化钴电化学传感器检测细胞释放的超氧阴离子[J]. 分析化学, 2020, 48(2): 224-232. doi: 10.19756/j.issn.0253-3820.191600 shu

Citation: CUI Min, WEN Xiao-Fang, REN Ju-Jie, ZHANG Cong, LI Na, HAN Yuan-Yuan, YU Xu-Dong, JI Xue-Ping. Electrochemical Detection of Superoxide Anion Released from Living Cells Using Nano Cobalt Ferricyanide[J]. Chinese Journal of Analytical Chemistry, 2020, 48(2): 224-232. doi: 10.19756/j.issn.0253-3820.191600 shu

纳米铁氰化钴电化学传感器检测细胞释放的超氧阴离子

崔敏 ^1, ,
温晓芳 ^1, ,
任聚杰 ^1, ,
张聪 ^1, ,
李娜 ^1, ,
韩元元 ^1, ,
余旭东 ^1, ,
籍雪平 ^2,

1.
河北科技大学理学院化学系, 石家庄 050018;
2.
河北医科大学药学院, 石家庄 050017
基金项目:
本文系国家自然科学基金项目（No.81872669）与河北省高等学校科学技术研究项目（Nos.ZD2018037，QN2019140）资助

摘要: 采用共沉淀方法制备的铁氰化钴（Co₃[Fe（CN）₆]₂）和Hummers法制备的还原氧化石墨烯（RGO）共沉积修饰玻碳电极（GCE），制备了无酶型超氧阴离子（O₂^·-）电化学传感器（Co₃[Fe（CN）₆]₂-RGO/GCE）。采用扫描电子显微镜（SEM）、X射线衍射（XRD）、电化学交流阻抗法（EIS）、循环伏安法（CV）等研究了复合材料Co₃[Fe（CN）₆]₂-RGO的特征及其对水中O₂^·-的电化学检测的可行性，分析了Co₃[Fe（CN）₆]₂-RGO/GCE检测O₂^·-的作用机理。传感器对O₂^·-浓度的检测范围为0.5~30 μmol/L，灵敏度为91.8 μA L/（μmol cm²），检出限为0.071 μmol/L（S/N=3），而且具有良好的选择性，H₂O₂、O₂及其它体内电化学活性物质均不干扰O₂^·-的测定，成功地应用于MCF-7细胞中释放的O₂^·-的电化学检测。此传感器在病理和生物学领域定量测定O₂^·-方面具有潜在的应用价值。

关键词:

English

Electrochemical Detection of Superoxide Anion Released from Living Cells Using Nano Cobalt Ferricyanide

1.
Department of Chemistry, School of Sciences, Hebei University of Science and Technology, Shijiazhuang 050018, China;
2.
Department of Medical Chemistry, Hebei Medical University, Shijiazhuang 050017, China
Received Date: 12 October 2019
Revised Date: 06 December 2019
Fund Project:
This work was supported by the National Natural Science Foundation of China (No. 81872669) and the Research Foundation of Education Department of Hebei Province of China (Nos. ZD2018037, QN2019140).

Abstract: A non-enzymatic superoxide anion (O₂^·-) electrochemical sensor (Co₃[Fe(CN)₆]₂-RGO/GCE) was fabricated by co-depositing cobalt ferricyanide (Co₃[Fe(CN)₆]₂) prepared by coprecipitation method and reduced graphene oxide (RGO) prepared by Hummers method on a glassy carbon electrode (GCE). The characteristics of Co₃[Fe(CN)₆]₂-RGO nanocomposites and the possibility for electrochemical detection of O₂^·- were studied using scanning electron microscopy (SEM), X-ray diffraction (XRD), electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). The mechanism of Co₃[Fe(CN)₆]₂-RGO for O₂^·- detection was investigated in this work. The sensor had a sensitivity of 91.8 μA L/(μmol cm²) and a detection limit of 0.071 μmol/L (S/N=3). Besides, it had excellent selectivity and could avoid the interference of hydrogen peroxide, oxygen and other electrochemical active substances in vivo. It was successfully applied to the electrochemical detection of superoxide anions in stimulated MCF-7 cells. The sensor had potential application for quantifying superoxide anions in the field of pathology and biology.

Key words:

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沈阳化工大学材料科学与工程学院沈阳 110142

纳米铁氰化钴电化学传感器检测细胞释放的超氧阴离子

English

Electrochemical Detection of Superoxide Anion Released from Living Cells Using Nano Cobalt Ferricyanide

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中国化学会秘书处

纳米铁氰化钴电化学传感器检测细胞释放的超氧阴离子

English

Electrochemical Detection of Superoxide Anion Released from Living Cells Using Nano Cobalt Ferricyanide

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

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CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

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