基于DNA QDs@PDA荧光共振能量转移的半胱氨酸传感器

引用本文: 陈飘飘, 邢怡晨, 刘洋, 郑姗, 黄朝表. 基于DNA QDs@PDA荧光共振能量转移的半胱氨酸传感器[J]. 分析化学, 2020, 48(1): 83-89. doi: 10.19756/j.issn.0253-3820.191576 shu

Citation: CHEN Piao-Piao, XING Yi-Chen, LIU Yang, ZHENG Shan, HUANG Chao-Biao. DNA Quantum Dots@Polydopamine as a Fluorescent Sensor for Cysteine Detection Based on Fluorescence Resonance Energy Transfer Effect[J]. Chinese Journal of Analytical Chemistry, 2020, 48(1): 83-89. doi: 10.19756/j.issn.0253-3820.191576 shu

基于DNA QDs@PDA荧光共振能量转移的半胱氨酸传感器

陈飘飘 ^1, ,
邢怡晨 ^1, ,
刘洋 ^1, ,
郑姗 ^1, ,
黄朝表 ^1,2,

1.
浙江师范大学化学与生命科学学院, 金华 321004;
2.
浙江师范大学行知学院, 兰溪 321100
基金项目:
本文系国家自然科学基金项目（No.21575129）资助

摘要: 建立了基于DNA量子点（DNA QDs）与聚多巴胺（PDA）荧光共振能量转移（FRET）检测半胱氨酸的方法。DNA QDs发射的荧光被PDA分子吸收，发生FRET，导致DNA QDs的荧光猝灭，使DNA QDs处于荧光"关闭"状态。当存在半胱氨酸时，从多巴胺（DA）向PDA的自发氧化聚合反应将被阻断，使DNA QDs的荧光恢复，处于荧光"开启"状态，且DNA QDs荧光的恢复程度与溶液中半胱氨酸的浓度相关，基于此构建了半胱氨酸荧光传感器。检测半胱氨酸的线性方程为y=0.0181x-0.0185，线性范围为10.0~100.0 μmol/L，检出限为1.7 μmol/L（S/N=3，n=10），此传感器对半胱氨酸具有良好的选择性，常见氨基酸及生物硫醇小分子均无干扰。将本方法用于人尿样中半胱氨酸的测定，回收率为98.6%~105.9%。

关键词:

English

DNA Quantum Dots@Polydopamine as a Fluorescent Sensor for Cysteine Detection Based on Fluorescence Resonance Energy Transfer Effect

1.
College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, China;
2.
Xingzhi College, Zhejiang Normal University, Lanxi 321100, China
Received Date: 28 September 2019
Revised Date: 10 November 2019
Fund Project:
This work was supported by the National Natural Science Foundation of China (No.21575129).

Abstract: A method for detecting cysteine based on fluorescenceresonance energy transfer (FRET) between DNA quantum dots (QDs) and polydopamine (PDA) was developed. Since the fluorescence emitted by DNA QDs was absorbed by the PDA molecule, FRET occurred, resulting in fluorescence quenching of DNA QDs, and leaving the DNA QDs in a fluorescent "off" state. In the presence of cysteine, the spontaneous oxidative polymerization from dopamine (DA) to PDA was blocked, the fluorescence of DNA QDs was restored, and the fluorescence was "on" state. Based on this, a cysteine fluorescence sensor was developed. The sensor had good selectivity to cysteine, and there was no interference between common amino acids and small biothiol molecules. The linear equation was y=0.0181x-0.0185 in linear range of 10.0-100.0 μmol/L. The limit of detection (LOD) was 1.7 μmol/L (S/N=3). The method was successfully applied to determination of cysteine in human urine samples, with recoveries of 98.6%-105.9%.

Key words:

DNA quantum dots
/ Fluorescence resonance energy transfer
/ Polydopamine
/ Cysteine

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基于DNA QDs@PDA荧光共振能量转移的半胱氨酸传感器

English

DNA Quantum Dots@Polydopamine as a Fluorescent Sensor for Cysteine Detection Based on Fluorescence Resonance Energy Transfer Effect

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