采用固相萃取结合高效液相色谱测定清香型白酒中酚类活性化合物

引用本文: 吴子阳, 何菲, 李贺贺, 孙金沅, 孙啸涛, 孙宝国. 采用固相萃取结合高效液相色谱测定清香型白酒中酚类活性化合物[J]. 分析化学, 2020, 48(10): 1400-1408. doi: 10.19756/j.issn.0253-3820.191565 shu

Citation: WU Zi-Yang, HE Fei, LI He-He, SUN Jin-Yuan, SUN Xiao-Tao, SUN Bao-Guo. Determination of Phenolic Active Compounds in Light-aroma Baijiu by Solid Phase Extraction-High Performance Liquid Chromatography[J]. Chinese Journal of Analytical Chemistry, 2020, 48(10): 1400-1408. doi: 10.19756/j.issn.0253-3820.191565 shu

采用固相萃取结合高效液相色谱测定清香型白酒中酚类活性化合物

1.
北京工商大学食品质量与安全北京实验室, 北京 100048;
2.
北京工商大学食品营养与人类健康北京高精尖创新中心, 北京 100048
基金项目:
本文系国家自然科学基金项目（No.31701567）和国家重点研发计划项目（No.2016YFD0400500）资助

摘要: 建立了固相萃取结合高效液相色谱法（Solid phase extraction-high performance liquid chromatography，SPE-HPLC）对白酒中阿魏酸、没食子酸、对香豆酸、丁香酸和（+）-儿茶素水合物5种酚类活性物质的定性与定量分析方法，并对17种青稞酒原酒和29种商品酒中5种酚类活性化合物进行了测定。优化了前处理条件及色谱条件，发现当样品pH=2、甲醇洗脱溶液pH=4、甲醇洗脱溶液用量6 mL时，萃取效果较好。5种酚类活性化合物标准曲线线性良好（R²> 0.994），检出限为0.98-12.2 μg/L，定量限为3.3-40.1 μg/L，回收率为84.1%-100.7%。通过聚类分析，结合5种酚类活性化合物的含量，发现贮酒容器不同导致青稞酒原酒中5种酚类活性化合物含量不同，可以为白酒老熟后续的研究提供理论依据。青稞酒中5种酚类活性化合物的平均含量高于其它清香型商品酒，酱香型白酒中（+）-儿茶素水合物和对香豆酸的含量较高，芝麻香型白酒中阿魏酸的含量较高。

关键词:

English

Determination of Phenolic Active Compounds in Light-aroma Baijiu by Solid Phase Extraction-High Performance Liquid Chromatography

1.
Beijing Laboratory for Food Quality and Safety, Beijing Technology and Business University, Beijing 100048, China;
2.
Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University, Beijing 100048, China
Received Date: 21 September 2019
Revised Date: 22 July 2020
Fund Project:
This work was supported by the National Natural Science Foundation of China (No. 31701567) and the National Key R&D Program of China (No. 2016YFD0400500).

Abstract: An efficient and rapid method for qualitative and quantitative determination of ferulic acid, gallic acid, p-coumaric acid, syringic acid and (+)-catechin hydrate in baijiu by solid phase extraction-high performance liquid chromatography (SPE-HPLC) was established. The pre-treatment conditions and chromatographic conditions were optimized. Under the optimal conditions including sample pH=2, methanol elution solution pH=4, and 6 mL of methanol elution solution, the contents of 5 phenolic active compounds in 17 different green barley base baijiu and 29 kinds of other type commercial baijiu were determined. This method showed a good linearity for the 5 phenolic active compounds and the linear correlation coefficients (R²) were 0.9945-0.9999. Besides, the limits of detection were 0.98-12.2 μg/L, and the recoveries were 84.1%-100.7%. Through chemometrics such as cluster analysis, combined with the content of 5 phenolic active compounds, different baijiu in different baijiu storage containers could be distinguished. The average content of 5 phenolic active compounds in green barley baijiu was higher than most of other light-aroma commercial baijiu. The total content of 5 phenolic active compounds in four kinds of aroma baijiu was sauce-aroma> light-aroma> sesame-aroma> strong-aroma. Among them, the content of p-coumaric acid and (+)-catechin hydrate in the sauce-aroma baijiu was the highest, and the content of ferulic acid in the sesame-aroma baijiu was the highest.

Key words:

Baijiu
/ Green barley baijiu
/ Solid phase extraction-high performance liquid chromatography
/ Phenolic active compounds

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沈阳化工大学材料科学与工程学院沈阳 110142

采用固相萃取结合高效液相色谱测定清香型白酒中酚类活性化合物

English

Determination of Phenolic Active Compounds in Light-aroma Baijiu by Solid Phase Extraction-High Performance Liquid Chromatography

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

采用固相萃取结合高效液相色谱测定清香型白酒中酚类活性化合物

English

Determination of Phenolic Active Compounds in Light-aroma Baijiu by Solid Phase Extraction-High Performance Liquid Chromatography

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

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CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

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