基于氮掺杂石墨烯量子点/硫化镉纳米晶电化学发光传感器检测硫化氢

引用本文: 杜方凯, 张慧, 谭学才, 冯德芬, 罗燕妮. 基于氮掺杂石墨烯量子点/硫化镉纳米晶电化学发光传感器检测硫化氢[J]. 分析化学, 2020, 48(2): 240-247. doi: 10.19756/j.issn.0253-3820.191559 shu

Citation: DU Fang-Kai, ZHANG Hui, TAN Xue-Cai, FENG De-Fen, LUO Yan-Ni. Detection of Hydrogen Sulfide Based on Nitrogen-doped Graphene Quantum Dots/Cadmium Sulfide Nanocrystals Electrochemiluminescence Sensor[J]. Chinese Journal of Analytical Chemistry, 2020, 48(2): 240-247. doi: 10.19756/j.issn.0253-3820.191559 shu

基于氮掺杂石墨烯量子点/硫化镉纳米晶电化学发光传感器检测硫化氢

广西民族大学化学化工学院, 广西高校食品安全与药物分析化学重点实验室, 广西林产化学与工程重点实验室, 南宁 530006
基金项目:
本文系国家自然科学基金项目（No.11904059）、广西自然科学基金项目（Nos.2017GXNSFBA198026，2018GXNSFAA294044，2018GXNSFAA281136）、广西重点研发计划项目（No.AB18126048）、广西中青年教师基础能力提升项目（No.2018KY0168）和南京大学生命分析化学国家重点实验室项目（No.SKLACL1810）资助

摘要: 以柠檬酸和氨水为原料，用水热法制备了氮掺杂石墨烯量子点（NGQDs），与硫化镉纳米晶（CdS NCs）复合，构建了固态电化学发光（ECL）传感器，用于硫化氢（H₂S）的检测。采用紫外-可见吸收光谱和荧光光谱对NGQDs和CdS NCs进行了表征，同时对传感器的ECL和电化学行为进行了系统研究。结果表明，以H₂O₂为CdS NCs的共反应试剂时，NGQDs可增强CdS NCs的ECL信号，并且NGQDs/CdS NCs的稳定性增加。H₂S存在下，S^2-与过量的Cd²⁺发生键合作用，ECL值降低。在最优条件下，ECL变化值与H₂S浓度（2.0×10^-10~2.0×10^-5 mol/L）的对数呈良好的线性关系，检出限为6.7×10^-11 mol/L。采用本传感器测定血清中H₂S浓度，加标回收率为92.7%~103.8%。

关键词:

English

Detection of Hydrogen Sulfide Based on Nitrogen-doped Graphene Quantum Dots/Cadmium Sulfide Nanocrystals Electrochemiluminescence Sensor

School of Chemistry and Chemical and Engineering, Guangxi University for Nationalities, Key Laboratory of Guangxi Colleges and Universities for Food Safety and Pharmaceutical Analytical Chemistry, Guangxi Key Laboratory of Chemistry and Engineering of Forest Products, Nanning 530006, China
Received Date: 18 September 2019
Revised Date: 18 November 2019
Fund Project:
This work was supported by the National Natural Science Foundation of China (No. 11904059), the Natural Science Foundation of Guangxi Province, China (Nos. 2017GXNSFBA198026, 2018GXNSFAA294044, 2018GXNSFAA281136), the Key Research and Development Project of Guangxi Province, China (No. AB18126048), the Basic Ability Enhancement Program for Young and Middle-aged Teachers of Guangxi Province, China (No. 2018KY0168), and the State Key Laboratory of Analytical Chemistry for Life Science, Nanjing University (No. SKLACL1810).

Abstract: Nitrogen-doped graphene quantum dots (NGQDs) were synthesized by hydrothermal method with citric acid and ammonia as raw materials. A solid-state electrochemiluminescence (ECL) sensor was thus constructed by dropping NGQDs/CdS nanocluster (NCs) composites. The properties of NGQDs and CdS NCs were characterized by UV-Vis absorption spectroscopy and fluorescence spectroscopy. The electrochemiluminescent and electrochemical behaviors of the sensor were studied. The results showed that NGQDs could enhance electrochemiluminescence signal of CdS NCs and the stability of NGQDs/CdS NCs increased when H₂S was used as the co-reaction reagent of CdS NCs. In the presence of H₂S, S^2- bonded with excess Cd²⁺, and the ECL intensity decreased. Under the optimal conditions, the decrease in electrochemiluminescence intensity varied proportionally with the logarithmic concentration of H₂S from 2.0×10^-10-2.0×10^-5 mol/L, and the detection limit was estimated to be 6.7×10^-11 mol/L. The sensor was used for the determination of H₂S in bovine serum with recoveries of 92.7%-103.8%, which indicated that the proposed sensor had the potential for detection of H₂S in practical complex samples.

Key words:

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沈阳化工大学材料科学与工程学院沈阳 110142

基于氮掺杂石墨烯量子点/硫化镉纳米晶电化学发光传感器检测硫化氢

English

Detection of Hydrogen Sulfide Based on Nitrogen-doped Graphene Quantum Dots/Cadmium Sulfide Nanocrystals Electrochemiluminescence Sensor

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基于氮掺杂石墨烯量子点/硫化镉纳米晶电化学发光传感器检测硫化氢

English

Detection of Hydrogen Sulfide Based on Nitrogen-doped Graphene Quantum Dots/Cadmium Sulfide Nanocrystals Electrochemiluminescence Sensor

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

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CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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