基于石墨烯和金纳米粒子的铜离子配位-分子印迹电化学传感器用于凝血酶的检测

引用本文: 柏朝朋, 杨绍明, 滕渝, 张剑. 基于石墨烯和金纳米粒子的铜离子配位-分子印迹电化学传感器用于凝血酶的检测[J]. 分析化学, 2020, 48(1): 137-144. doi: 10.19756/j.issn.0253-3820.191543 shu

Citation: BAI Chao-Peng, YANG Shao-Ming, TENG Yu, ZHANG Jian. Detection of Thrombin by Copper Ion Coordination-Molecular Imprinted Electrochemical Sensor Based on Graphene and Gold Nanoparticles[J]. Chinese Journal of Analytical Chemistry, 2020, 48(1): 137-144. doi: 10.19756/j.issn.0253-3820.191543 shu

基于石墨烯和金纳米粒子的铜离子配位-分子印迹电化学传感器用于凝血酶的检测

华东交通大学材料学院, 南昌 330013
基金项目:
本文系国家自然科学基金项目（No.21465012）资助

摘要: 利用分子印迹技术与铜离子（Cu²⁺）配位单元为双重识别模式，使用层层自组装先将L-半胱氨酸（L-cys）通过AuS键组装到石墨烯（rGO）和金纳米粒子（AuNPs）修饰的玻碳电极表面，再将Cu-凝血酶（THR）络合物通过L-cys与Cu²⁺配位作用组装到电极表面，以硫堇（Tn）为聚合单体，使用电聚合法聚合得到分子印迹膜，制备了具有双重识别模式的金属离子配体-分子印迹电化学传感器。采用扫描电镜（SEM）和能谱色散仪（EDS）对复合纳米材料进行表征。利用循环伏安法（CV）、交流阻抗法（EIS）和差分脉冲伏安法（DPV）对传感器性能进行了研究，在最佳检测条件下，传感器响应与THR浓度在2.0×10^-9~5.0×10^-7 g/L范围内呈良好的线性关系，线性方程为-ΔI（μA）=17.73+1.84 lgc（g/L）。构建了THR分子印迹电化学传感器的动力学吸附模型，测得印迹传感器的印迹因子β=4.32，结合速率常数k=5.68 s。传感器表现出良好的稳定性和重现性，可用于实际样品中凝血酶的检测。

关键词:

English

Detection of Thrombin by Copper Ion Coordination-Molecular Imprinted Electrochemical Sensor Based on Graphene and Gold Nanoparticles

School of Materials Science and Engineering, East China Jiaotong University, Nanchang 330013, China
Received Date: 28 September 2019
Revised Date: 14 November 2019
Fund Project:
This work was supported by the National Natural Science Foundation of China (No. 21465012).

Abstract: A metal ion ligands and molecular imprinted electrochemical sensors with double recognition patterns were prepared using molecular imprinting technique and with copper ion coordination unit as the double recognition mode. L-cystine (L-cys) was firstly assembled on the surface of graphene (rGO) and gold nanoparticles (AuNPs) modified glassy carbon electrode by AuS bond, and then the Cu-THR complex was assembled on the electrode surface by the coordination of L-cys and thrombin (Tn) by layer-by-layer self-assembly method. Thionine was used as monomer and electropolymerization method was used to form the imprinted membrane. The composite nanomaterials were characterized by scanning electron microscopy (SEM) and energy dispersive spectroscopy (EDS). The performance of the sensor was studied by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV). Under the optimum conditions, the sensor showed an excellent linear relationship with thrombin concentration in the range of 2.0×10^-9-5.0×10^-7 g/L. The linear equation was -ΔI (μA)=17.73+1.84lgc (g/L). The kinetic adsorption model of THR molecularly imprinted electrochemical sensor was investigated, and the measured imprinting factor β of the imprinted sensor was 4.32, with the binding rate constant k of 5.68 s. The sensor had good stability and reproducibility, and could be used to detect thrombin in practice.

Key words:

Graphene
/ Gold nanoparticles
/ Thrombin
/ Double recognition mode
/ Molecular imprinted electrochemical sensor

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基于石墨烯和金纳米粒子的铜离子配位-分子印迹电化学传感器用于凝血酶的检测

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Detection of Thrombin by Copper Ion Coordination-Molecular Imprinted Electrochemical Sensor Based on Graphene and Gold Nanoparticles

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