分散固相萃取-增强型去除脂质技术结合超高效液相色谱-串联质谱法测定塑料包装焙烤食品中12种抗氧化剂和紫外吸收剂

引用本文: 黄宇锋, 温少楷, 王莉, 冼燕萍, 郑慷, 王斌, 梁明, 吴玉銮, 胡均鹏, 陈荣桥. 分散固相萃取-增强型去除脂质技术结合超高效液相色谱-串联质谱法测定塑料包装焙烤食品中12种抗氧化剂和紫外吸收剂[J]. 分析化学, 2020, 48(7): 937-945. doi: 10.19756/j.issn.0253-3820.191506 shu

Citation: HUANG Yu-Feng, WEN Shao-Kai, WANG Li, XIAN Yan-Ping, ZHENG Kang, WANG Bin, LIANG Ming, WU Yu-Luan, HU Jun-Peng, CHEN Rong-Qiao. Determination of 12 Kinds of Antioxidants and Ultraviolet Absorbers in Plastic Packaging Baked Foods by QuEChERS Enhanced Matrix Removal-Lipid Combined with Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, 2020, 48(7): 937-945. doi: 10.19756/j.issn.0253-3820.191506 shu

分散固相萃取-增强型去除脂质技术结合超高效液相色谱-串联质谱法测定塑料包装焙烤食品中12种抗氧化剂和紫外吸收剂

广州质量监督检测研究院, 广州市食品安全风险动态监测与预警研究中心, 广州市食品安全检测技术重点实验室, 广州 511447
基金项目:
本文系广东省科技计划项目（No.2019B020208008）资助

摘要: 建立了QuEChERS EMR-Lipid技术结合超高效液相色谱-串联三重四极杆质谱（UPLC-MS/MS）同时检测塑料包装焙烤食品中4种抗氧化剂和8种紫外吸收剂的方法。样品经乙腈-丙酮（1：1，V/V）提取，QuEChERS EMR-Lipid技术净化，BEH C₁₈色谱柱分离，甲醇-10 mmol/L乙酸铵梯度洗脱，串联质谱电喷雾正、负离子扫描，多反应监测（MRM）模式检测，采用外标法进行定量分析。结果表明，12种目标物在一定的浓度范围内线性关系良好（R²>0.9978），方法检出限（S/N=3）在5.0~10.0 μg/kg之间（除Antioxidant 2246的检出限为150.0 μg/kg外），回收率为80.3%~118.5%，日内精密度RSD（n=6）为1.7%~9.8%，日间精密度RSD（n=5）为4.5%~9.9%。本方法前处理简单快速、净化效果好、灵敏度高，适用于塑料包装焙烤食品中抗氧化剂和紫外吸收剂的检测。

关键词:

English

Determination of 12 Kinds of Antioxidants and Ultraviolet Absorbers in Plastic Packaging Baked Foods by QuEChERS Enhanced Matrix Removal-Lipid Combined with Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry

Guangzhou Quality Supervision and Testing Institute, Guangzhou City Research Center of Risk Dynamic Detection and Early Warning for Food Safety, Guangzhou City Key Laboratory of Detection Technology for Food Safety, Guangzhou 511447, China
Received Date: 20 August 2019
Revised Date: 20 March 2020
Fund Project:
This work was supported bythe Science and Technology Project of Guangdong Province, China (No. 2019B020208008).

Abstract: An effective method was developed for the simultaneous determination of 4 kinds of antioxidants and 8 kinds of the ultraviolet absorbers in plastic packaging baked foods by QuEChERS enhanced matix removal-lipid (EMR-Lipid) combined with ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The samples were extracted by acetonitrile-acetone (1:1, V/V). The 12 targets were separated on a Waters BEH C₁₈ column by gradient elution with methanol-10mmol/L ammonium acetate as mobile phase, ionized with positive and negative electrospray ionization (ESI⁺/-), detected under multiple reaction monitoring mode (MRM), and quantified with external standard method. The result showed that the 12 kinds of analytes presented favorable linearity in the respective linear range, with correlation coefficients (R²) of more than 0.9978. The method detection limits (S/N=3) were in the range of 5.0-10.0 μg/kg, except for the Antioxidant 2246 was 150.0 μg/kg. The average recoveries (n=6) ranged from 80.3% to 118.5%, with intra-day relative standard deviations (RSD, n=6) of 1.7%-9.8% and inter-day RSD (n=5) of 4.5%-9.9%. The developed method had simple pre-treatment procedure, satisfactory purification and sensitivity, and was suitable for the simultaneous determination of antioxidants and ultraviolet absorbers in plastic packaging baked foods.

Key words:

Antioxidants
/ Ultraviolet absorbers
/ Baked foods
/ Ultra-high performance liquid chromatography-tandem mass spectrometry

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分散固相萃取-增强型去除脂质技术结合超高效液相色谱-串联质谱法测定塑料包装焙烤食品中12种抗氧化剂和紫外吸收剂

English

Determination of 12 Kinds of Antioxidants and Ultraviolet Absorbers in Plastic Packaging Baked Foods by QuEChERS Enhanced Matrix Removal-Lipid Combined with Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry

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中国化学会秘书处

分散固相萃取-增强型去除脂质技术结合超高效液相色谱-串联质谱法测定塑料包装焙烤食品中12种抗氧化剂和紫外吸收剂

English

Determination of 12 Kinds of Antioxidants and Ultraviolet Absorbers in Plastic Packaging Baked Foods by QuEChERS Enhanced Matrix Removal-Lipid Combined with Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

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