Sin-QuEChERS结合超高效液相色谱-高分辨质谱法快速筛查绿茶中农药及代谢物残留

黄合田 谢双 涂祥婷 杨鸿波 郭峰 孙晓红 段亚玲 李占彬

引用本文: 黄合田,  谢双,  涂祥婷,  杨鸿波,  郭峰,  孙晓红,  段亚玲,  李占彬. Sin-QuEChERS结合超高效液相色谱-高分辨质谱法快速筛查绿茶中农药及代谢物残留[J]. 分析化学, 2020, 48(3): 423-430. doi: 10.19756/j.issn.0253-3820.191503 shu
Citation:  HUANG He-Tian,  XIE Shuang,  TU Xiang-Ting,  YANG Hong-Bo,  GUO Feng,  SUN Xiao-Hong,  DUAN Ya-Ling,  LI Zhan-Bin. Rapid Screening of Pesticide and Metabolites Residues in Green Tea by Sin-QuEChERS with Ultra Performance Liquid Chromatography-High Resolution Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, 2020, 48(3): 423-430. doi: 10.19756/j.issn.0253-3820.191503 shu

Sin-QuEChERS结合超高效液相色谱-高分辨质谱法快速筛查绿茶中农药及代谢物残留

  • 基金项目:

    本文系国家国际科技合作专项(No.2015DFA41280)、国家自然科学基金项目(No.201966011)、黔科合支撑项目(No.[2016]2578)和中央级公益性科研院所基本科研业务费项目(No.CSJ201906)资助

摘要: 建立了Sin-QuEChERS结合超高效液相色谱-四极杆-静电场轨道阱高分辨质谱法快速非靶向筛查绿茶中农药残留的分析方法。以38种农药及代谢物为质控化合物,优化了分析方法。样品经0.1%甲酸-乙腈溶液提取/盐析、振荡并离心,经Sin-QuEChERS Nano净化柱单步净化,以Thermo Accucore aQ色谱柱(150 mm×2.1 mm,2.6 μm)进行分离,以甲醇和0.1%甲酸-水(含5 mmol/L甲酸铵)为流动相,梯度洗脱。在正离子模式下,以Full MS/ddMS2扫描模式进行分析,各物质的精确分子量偏差小于3.8×10-6,38种农药及代谢物在各自的线性范围内线性关系良好(R>0.99)。在0.01、0.020和0.05 mg/kg加标水平下,除乙硫甲威和蝇毒磷,各物质的平均回收率为68%-115%,相对标准偏差(RSD)为0.4%-18.9%,定量限(LOQ)为0.005-0.02 mg/kg。采用本方法非靶向快速筛查绿茶样品的残留农药,并定量分析残留水平,共检出10种残留农药。本方法处理过程简便、快速、高效、准确,可用于绿茶中多种农药残留及代谢物的快速筛查。

English


    1. [1]

      Yang C S, Landau J M. J. Nutrition,2000,130(10):2409-2412

    2. [2]

      YU Lu, SONG Wei, LU Ya-Ning, ZHAO Mu-Yu, ZHOU Fang-Fang, HU Yan-Yun, ZHENG Ping. Chinese Journal of Chromatography,2015,33(6):597-612 余璐, 宋伟, 吕亚宁, 赵暮雨, 周芳芳, 胡艳云, 郑平.色谱,2015,33(6):597-612

    3. [3]

      Huang Z, Zhang Y, Wang L, Ding L, Wang M, Yan H, Li Y, Zhu S. J. Sep. Sci.,2009,32(9):1294-1301

    4. [4]

      GB/T 2763-2019, National Food Safety Standard-Maximum Residue Limits of Pesticides in Foods. National Standard of the People's Republic of china.食品安全国家标准-食品中农药最大残留限量.中华人民共和国国家标准. GB/T 2763-2019

    5. [5]

      Huang Y, Shi T, Luo X, Hiong H, Min F, Chen Y, Nie S, Xie M. Food Chem.,2019,275:255-264

    6. [6]

      Wang J, Chow W, Leung D. J. AOAC Int.,2011,94(6):1685-1714

    7. [7]

      Wang S, Liu Y, Jiao S, Zhao Y, Guo Y, Wang M, Zhu G. J. Agr. Food Chem.,2017,65(46):10107-10114

    8. [8]

      Chen Q, Hassan M M, Xu J, Zareef M, Li H, Xu Y, Wang P, Agyekum A A, Kutsanedzie F Y H, Viswadevarayalu A. Spectrochim. Acta A,2019,211:86-93

    9. [9]

      SU Jian-Feng, ZHONG Mao-Sheng, CHEN Jing, GUO Xi, CHEN Jin-Xing, LIANG Zhen, LIU Jian-Jun. Journal of Instrumental Analysis,2015,34(6):625-638 苏建峰, 钟茂生, 陈晶, 郭昕, 陈劲星, 梁震, 刘建军.分析测试学报,2015,34(6):625-638

    10. [10]

      Zhu B, Xu X, Luo J, Jin S, Chen W, Liu Z, Tian C. Food Chem.,2019,276:202-208

    11. [11]

      GAO Yi-Xian, CHEN Ping-Hong, NIE Dan-Dan. Chinese Journal of Chromatography,2018,36(6):531-540 高艺羡, 陈萍虹, 聂丹丹.色谱,2018,36(6):531-540

    12. [12]

      HUANG Wei, LI Na, XU Rui-Han, LI Ting, LI Chong-Yong. Chinese Journal of Chromatography,2018,36(12):1303-1310 黄微, 李娜, 徐瑞晗, 李婷, 李崇勇.色谱,2018,36(12):1303-1310

    13. [13]

      Jiao W, Xiao Y, Qian X, Tong M, Hu Y, Hou R, Hua R. Food Chem.,2016,210:26-34

    14. [14]

      Xie W, Han C, Qian Y, Ding H, Chen X, Xi J. Food Chem.,2019,275:255-264

    15. [15]

      Saito-Shida S, Hamasaka T, Nemoto S, Akiyama H. Food Chem.,2018,256:140-148

    16. [16]

      Dzuman Z, Zachariasova M, Veprikova Z, Godula M, Hajslova J. Anal. Chim. Acta,2015,863:29-40

    17. [17]

      WU Ying-Yi, CHEN Zhong, ZHANG Si-Qun, ZHANG Hong. Science and Technology of Food Industry,2019,40(15):188-195 伍颖仪, 陈中, 张思群, 张弘.食品工业科技,2019,40(15):188-195

    18. [18]

      Gómez-RamosMaría M D, Rajski L, Horacio H, Fernández-Alba A R. Anal. Bioanal. Chem.,2015,407(21):6317-6326

    19. [19]

      Ma G, Zhang M, Zhu L, Chen H, Liu X, Lu C. J. Chromatogr. A,2017,1531:22-31

    20. [20]

      CHENG Jing, WANG Mei-Ling, ZHU Shao-Hua, WEN Jiang-Wei, DING Li, WANG Li-Bing. Journal of Food Safety and Quality,2016,7(1):132-137 成婧, 王美玲, 朱绍华, 文江为, 丁利, 王利兵.食品安全质量检测学报,2016,7(1):132-137

    21. [21]

      Oellig C, Schwack W. J. Chromatogr. A,2014,1351:1-11

    22. [22]

      Wang F, Li S, Feng H, Yang Y, Xiao B, Chen D. Food Chem.,2019,275:530-538

    23. [23]

      GAO Yu-Ling, GUO Xiu-Juan, QIAO Shi, SUN Peng. Journal of Tea Science,2017,37(3):243-250 高玉玲, 郭秀娟, 乔石, 孙鹏.茶叶科学,2017,37(3):243-250

    24. [24]

      Zhao P, Wang L, Jiang Y, Zhang F, Pan C. J. Agr. Food Chem.,2012,60(16):4026-4033

    25. [25]

      Han Y, Song L, Zou N, Chen R, Qin Y, Pan C. J. Chromatogr. B,2016,1031:99-108

    26. [26]

      Han Y T, Zou N, Song L, Li Y J, Qin Y H, Liu S W, Li X S, Pan C P. J. Chromatogr.,2015,1005:56-64

    27. [27]

      LIU Han, ZHANG Ya-Lian, DING Tao, WANG Sui-Lou, XU Niu-Sheng, GUI Qian-Wen, SHEN Wei-Jian, ZHAO Zeng-Yun, WU Bin, SHEN Chong-Yu, ZHANG Rui. Journal of Instrumental Analysis,2014,33(5):489-498 柳菡, 张亚莲, 丁涛, 王岁楼, 徐牛生, 桂茜雯, 沈伟健, 赵增运, 吴斌, 沈崇钰, 张睿.分析测试学报,2014,33(5):489-498

    28. [28]

      WU Bin, DING Tao, LIU Han, CHEN Hui-Lan, ZHAO Zeng-Yun, ZHANG Rui, SHEN Chong-Yu. Chinese Journal of Chromatography,2012,30(12):1246-1252 吴斌, 丁涛, 柳菡, 陈惠兰,赵增运, 张睿, 沈崇钰.色谱,2012, 30(12):1246-1252

    29. [29]

      Chen H, Gao G, Chai Y, Ma G, Hao Z, Wang C, Liu X, Lu C. ACS Omega,2017,2(9):5917-5927

    30. [30]

      Fan S, Zhao P, Yu C, Pan C, Li X. Food Addit. Contam. A,2014,31(1):73-82

    31. [31]

      Song L, Han Y T, Yang J, Qin Y H, Zeng W B, Xu S Q, Pan C P. Food Chem.,2019,279:237-245

    32. [32]

      Chen J N, Lian Y J, Zhou Y R, Wang M H, Zhang X Q, Wang J H, Wu Y N, Wang M L. Molecules (Basel, Switzerland),2019,24(16):2918

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  • 收稿日期:  2019-08-15
  • 修回日期:  2019-12-10
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