三维超分辨荧光和形貌显微镜联用系统的研制

引用本文: 周露露, 蔡明军, 王慧利, 王宏达. 三维超分辨荧光和形貌显微镜联用系统的研制[J]. 分析化学, 2020, 48(2): 174-179. doi: 10.19756/j.issn.0253-3820.191502 shu

Citation: ZHOU Lu-Lu, CAI Ming-Jun, WANG Hui-Li, WANG Hong-Da. Development of Correlative Super-Resolution Fluorescence and Three-Dimensional Topography Imaging Microscopy[J]. Chinese Journal of Analytical Chemistry, 2020, 48(2): 174-179. doi: 10.19756/j.issn.0253-3820.191502 shu

三维超分辨荧光和形貌显微镜联用系统的研制

1.
中国科学院长春应用化学研究所, 长春 130022;
2.
中国科学院大学, 北京 100049;
3.
中国科学技术大学, 合肥 230027
基金项目:
本文系国家重点研发计划项目（No.2017YFA0505300）和国家自然科学基金项目（Nos.21727816，21721003）资助

摘要: 搭建了一种联用超分辨荧光和三维形貌显微成像系统（Correlative super-resolution fluorescence and three-dimensional topography imaging microscopy），以聚合纤维状肌动蛋白（F-actin）为例，评价了此联用系统的性能。实验结果表明，此联用仪器具有纳米级分辨率的三维形貌及具体成分的定位分布成像功能，可实现在形貌图中定位具体成分的功能，表明此联用仪器可应用于研究分析细胞骨架及细胞亚结构（如细胞膜蛋白质聚集体的组成和分布特性等）方面，此联用系统在生命科学和材料科学等领域具有广泛的应用前景。

关键词:

English

Development of Correlative Super-Resolution Fluorescence and Three-Dimensional Topography Imaging Microscopy

1.
Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China;
2.
University of Chinese Academy of Sciences, Beijing 100049, China;
3.
University of Science and Technology of China, Hefei 230027, China
Received Date: 15 August 2019
Revised Date: 20 December 2019
Fund Project:
This work was supported by the National Key R&D Program of China (No. 2017YFA0505300) and the National Natural Science Foundation of China (Nos. 21727816, 21721003).

Abstract: A correlative super-resolution fluorescence and three-dimensional (3D) topography imaging microscopy was built, and the performance of the correlative system was evaluated with F-actin as an example. The experimental results suggested that the correlative instrument had the abilities to provide 3D overall topography and distribution of specific components at nanometer resolution, as well as to correlate the specific components' distribution with the overall topography. Thus, the correlative instrument is a powerful tool to investigate events regarding cytoskeleton and cellular substructure such as the composition and distribution characteristics of membrane protein clusters, and can also be widely applied to other fields such as life science and material science.

Key words:

Super-resolution fluorescence microscopy
/ Atomic force microscopy
/ Correlation

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三维超分辨荧光和形貌显微镜联用系统的研制

English

Development of Correlative Super-Resolution Fluorescence and Three-Dimensional Topography Imaging Microscopy

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