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Citation: LIU Ya, ZHANG Bo. Microscale Sample Transfer Technology for Capillary Two-Dimensional Liquid Chromatography[J]. Chinese Journal of Analytical Chemistry, ;2019, 47(11): 1803-1808. doi: 10.19756/j.issn.0253-3820.191490 shu

Microscale Sample Transfer Technology for Capillary Two-Dimensional Liquid Chromatography

LIU Ya ,
ZHANG Bo

College of Chemistry & Chemical Engineering, Xiamen University, Xiamen 361005, China

Received Date: 11 August 2019
Revised Date: 6 September 2019

Fund Project: This work was supported by the National Natural Science Foundation of China (Nos. 21475110, 21535007, J1310024) and the Xiamen Municipal Science and Technology Plan Project (No. 3502Z20173019).

Hydrophilic interaction chromatography (HILIC) coupled with reverse phase liquid chromatography (RPLC) is the most commonly used two-dimensional separation mode. However, it is suffered from the incompatibility of mobile phase. To overcome this issue, a microscale sample transfer technology was developed for capillary HILIC-RPLC two-dimensional liquid chromatography in this work. The sample transfer technology was consisted of weak elution make-up liquid and trap column, so that the strong elution solvent from the first dimension could be diluted effectively. Through optimization of make-up flow rate, the organic concentration of the first dimension effluent was reduced to less than 5%, and up to 95% sample transfer efficiency was achieved. This technology was applied to the two-dimensional separation of five standard protein digest. When 8 sample fractions from the first dimension separation were transferred to the second dimension separation, a theoretical peak capacity of 680 could be obtained. In addition, the peak capacity could be improved with the increasing sampling frequency, suggesting that this technology could be effectively applied to high-resolution separation of complex mixtures.
- Two dimensional liquid chromatography,
- Hydrophilic interaction chromatography,
- Reverse phase chromatography,
- Sample transfer,
- Peak capacity
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Microscale Sample Transfer Technology for Capillary Two-Dimensional Liquid Chromatography

南开大学师唯/华北电力大学（保定）刘景维：二维配位聚合物中有序的亲锂冠醚位点用于无枝晶锂沉积

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通讯作者: 陈斌, bchen63@163.com