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Citation: WANG Xiao-Li, YAO Meng, LI Yin, WEI Chao, WANG Mei. A Novel Colorimetric and Fluorescent Probe for Detection of Hydrogen Sulfide[J]. Chinese Journal of Analytical Chemistry, ;2019, 47(12): 1915-1921. doi: 10.19756/j.issn.0253-3820.191451 shu

A Novel Colorimetric and Fluorescent Probe for Detection of Hydrogen Sulfide

WANG Xiao-Li ^1, ,
YAO Meng ^1,2 ,
LI Yin ¹ ,
WEI Chao ¹ ,
WANG Mei ^1,2

1.
College of Chemistry and Environmental Science, Hebei University, Baoding 071002, China;
2.
The Flame Retardant Material and Processing Technology Engineering Research Center of Hebei Province, Baoding 071002, China

Received Date: 28 July 2019
Revised Date: 4 November 2019

Fund Project: This work was supported by the Natural Science Foundation of Hebei Province, China (Nos. B2019201418, B2018201234), the Colleges and Universities Science Technology Research Project of Hebei Province, China (NO. QN2017015), and the Science Technology Research and Development Guidance Programme Project of Baoding City, China (NO. 18ZF315).

To detect the level of hydrogen sulfide (H₂S) in the environment and living beings, a colorimetric and fluorescent turn-off probe based on selective thiolyling of NBD (7-nitro-1,2,3-benzoxadiazole) amino was prepared through one-step organic synthesis. The selectivity and sensitivity of the probe were investigated by using sodium sulfide as an H₂S donor in PBS buffer. The fluorescent intensity of the probe exhibited good linear relationship with concentration of H₂S in the concentration range of 5-50 μmol/L, and the detection limit was estimated to be 0.125 μmol/L. The color of the solution changed from light yellow to light violet. The cell survival rate was higher than 90% when the concentration of the probe was below 50 μmol/L. The results of cell imaging experiments showed that the probe could be used for imaging H₂S in the lysosomes of the cells.
- Hydrogen sulfide,
- Colorimetric detection,
- Fluorescent probe,
- Cell imaging
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