基于碳点的适配体荧光传感器检测农药乐果

引用本文: 陆婷婷, 王金龙, 詹相强, 吴远根. 基于碳点的适配体荧光传感器检测农药乐果[J]. 分析化学, 2020, 48(1): 74-82. doi: 10.19756/j.issn.0253-3820.191442 shu

Citation: LU Ting-Ting, WANG Jin-Long, ZHAN Xiang-Qiang, WU Yuan-Gen. Carbon Dots-based Fluorescent Aptasensor for Detection of Dimethoate Pesticide[J]. Chinese Journal of Analytical Chemistry, 2020, 48(1): 74-82. doi: 10.19756/j.issn.0253-3820.191442 shu

基于碳点的适配体荧光传感器检测农药乐果

1.
贵州大学贵州省发酵工程与生物制药重点实验室, 酿酒与食品工程学院, 贵阳 550025;
2.
中国轻工业浓香型白酒固态发酵重点实验室, 宜宾 644000
基金项目:
本文系国家自然科学基金项目（Nos.21565009，31760486）、贵州省科学技术基金项目（No.黔科合基础[2016]1403号）、贵州省科技支撑计划项目（No.黔科合支撑[2018]2795号）和中国轻工业浓香型白酒固态发酵重点实验室开放基金（No.2018JJ002）资助

摘要: 构建了一种基于核酸适配体调控碳点荧光强度的农药乐果适配体荧光传感器。在乐果存在下，适配体SS24-S-35可改变纳米银（AgNPs）对柠檬酸-乙二胺碳点荧光的猝灭作用，且碳点的荧光猝灭程度与乐果浓度成正比，由此建立了一种乐果的适配体荧光检测方法。对荧光猝灭机制进行了探讨，对碳点种类、AgNPs浓度等影响因素进行了考察。在最优条件下，检测乐果的线性范围为6~200 μg/L（R²=0.984），检出限为2.24 μg/L（3σ/S）。此传感器特异性良好，其它农药对乐果的检测无明显干扰。将此适配体荧光传感器应用于实际农产品中乐果农药检测，回收率为85.1%~105.0%，表明在农药残留检测中具有潜在应用价值。

关键词:

English

Carbon Dots-based Fluorescent Aptasensor for Detection of Dimethoate Pesticide

1.
Guizhou Province Key Laboratory of Fermentation Engineering and Biopharmacy, School of Liquor and Food Engineering, Guizhou University, Guiyang 550025, China;
2.
Key Laboratory of Wuliangye-flavor Liquor Solid-state Fermentation, China National Light Industry, Yibin 644000, China
Received Date: 25 July 2019
Revised Date: 15 November 2019
Fund Project:
This work was supported by the National Natural Science Foundation of China (Nos.21565009, 31760486), the Natural Science Foundation of Guizhou Province (No.[2016]1403), the Science and Technology Support Program of Guizhou Province for Social Development (No.[2018]2795), and the Foundation of Key Laboratory of Wuliangye-flavor Liquor Solid-state Fermentation (No. 2018JJ002), China National Light Industry.

Abstract: A fluorescent aptasensor for detection of dimethoate pesticide based on aptamer regulated carbon dots was fabricated. In the presence of dimethoate, SS24-S-35 aptamer could alter the quenching efficiency of silver nanoparticles (AgNPs) on the fluorescence of carbon dots prepared from ethylenediamine and citric acid, and the degree of fluorescence quenching was proportional to the concentration of dimethoate molecules. Therefore, this strategy used the proposed aptasensor to quantitatively detect dimethoate pesticide by fluorescent assay. To improve the detection performance of the as-prepared aptasensor, the fluorescence quenching mechanism of AgNPs on carbon dots was fully discussed, and some parameters such as the types of carbon dots and the amount of AgNPs were investigated. Under optimal conditions, the aptasensor could detect dimethoate in a linear range from 6 μg/L to 200 μg/L (R²=0.984), with a limit of detection (LOD) as low as 2.24 μg/L (3σ/S). Moreover, the further studies confirmed that other pesticides had negligible effect on detection of dimethoate, indicating the excellent specificity of aptasensor for dimethoate detection. The aptasensor was succesfully used in detection of dimethoate pesticide in real agriculture products with recovery of 85.1%-105.0%, which indicated that the proposed aptasensor had potential application in the detection of pesticide residues.

Key words:

Pesticides detection
/ Aptamer
/ Silver nanoparticles
/ Carbon dots
/ Fluorescence resonance energy transfer

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沈阳化工大学材料科学与工程学院沈阳 110142

基于碳点的适配体荧光传感器检测农药乐果

English

Carbon Dots-based Fluorescent Aptasensor for Detection of Dimethoate Pesticide

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