基于碳点的适配体荧光传感器检测农药乐果

陆婷婷 王金龙 詹相强 吴远根

引用本文: 陆婷婷,  王金龙,  詹相强,  吴远根. 基于碳点的适配体荧光传感器检测农药乐果[J]. 分析化学, 2020, 48(1): 74-82. doi: 10.19756/j.issn.0253-3820.191442 shu
Citation:  LU Ting-Ting,  WANG Jin-Long,  ZHAN Xiang-Qiang,  WU Yuan-Gen. Carbon Dots-based Fluorescent Aptasensor for Detection of Dimethoate Pesticide[J]. Chinese Journal of Analytical Chemistry, 2020, 48(1): 74-82. doi: 10.19756/j.issn.0253-3820.191442 shu

基于碳点的适配体荧光传感器检测农药乐果

  • 基金项目:

    本文系国家自然科学基金项目(Nos.21565009,31760486)、贵州省科学技术基金项目(No.黔科合基础[2016]1403号)、贵州省科技支撑计划项目(No.黔科合支撑[2018]2795号)和中国轻工业浓香型白酒固态发酵重点实验室开放基金(No.2018JJ002)资助

摘要: 构建了一种基于核酸适配体调控碳点荧光强度的农药乐果适配体荧光传感器。在乐果存在下,适配体SS24-S-35可改变纳米银(AgNPs)对柠檬酸-乙二胺碳点荧光的猝灭作用,且碳点的荧光猝灭程度与乐果浓度成正比,由此建立了一种乐果的适配体荧光检测方法。对荧光猝灭机制进行了探讨,对碳点种类、AgNPs浓度等影响因素进行了考察。在最优条件下,检测乐果的线性范围为6~200 μg/L(R2=0.984),检出限为2.24 μg/L(3σ/S)。此传感器特异性良好,其它农药对乐果的检测无明显干扰。将此适配体荧光传感器应用于实际农产品中乐果农药检测,回收率为85.1%~105.0%,表明在农药残留检测中具有潜在应用价值。

English


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  • 收稿日期:  2019-07-25
  • 修回日期:  2019-11-15
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