Citation: HAN Zeng-Peng, SHI Xiang-Wei, YING Min, ZHENG Ning, LI Mei, ZHANG Zhi-Jian, WU Yang, WANG Hua-Dong, WANG Jie, HU Liang, JIA Fan, XU Fu-Qiang. Tools for Neural Circuit Tracing Based on Neurotropic Viruses[J]. Chinese Journal of Analytical Chemistry, ;2019, 47(10): 1639-1650. doi: 10.19756/j.issn.0253-3820.191428 shu

Tools for Neural Circuit Tracing Based on Neurotropic Viruses

HAN Zeng-Peng ^1,2, ,
SHI Xiang-Wei ^1,2 ,
YING Min ^1,2 ,
ZHENG Ning ^1,2 ,
LI Mei ¹ ,
ZHANG Zhi-Jian ¹ ,
WU Yang ¹ ,
WANG Hua-Dong ^1,2 ,
WANG Jie ^1,2 ,
HU Liang ¹ ,
JIA Fan ^1,2 ,
XU Fu-Qiang ^1,2

1.
Brain Research Center, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan 430071, China;
2.
University of Chinese Academy of Sciences, Beijing 100049, China

Received Date: 20 July 2019
Revised Date: 2 September 2019

Fund Project: This work was supported by the National Natural Science Foundation of China (Nos. 91632303, 31830035, 31771197, 31771156), the Strategic Priority Research Program (B) (No. XDBS01030200), and the National Basic Research Program of China (No. 2015CB755600).

The brain is probably the most complex system. Neurocircuits, formed by various types of neurons through synaptic connections, are the basis of brain functions, from the basic homeostasis, senses and perception, for learning, memory, decision-making, and consciousness. Therefore, it is of great significance to understand the component and working principle of neurocircuits, so as to recognize, utilize and protect the brain. Techniques for exploring the structure and function of neurocircuits include circuit-tracing, manipulating, imaging and data-analyzing. In this paper, we focus on the neurocircuit tracers based on neurotropic viruses and its application technology. The categories and characteristics, genetic modification, selection principles and application limitations of commonly used neurotropic viral tools will be discussed. The non-transsynaptic tracer system, the trans-monosynaptic tracer system and the trans-multisynaptic tracer system are also introduced to help readers understand the commonly used circuit tracing methods and research progress.
- Neural circuit,
- Tools based on neurotropic virus,
- Retrograde,
- Anterograde,
- Helper virus,
- Review
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